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Composite bacterial agent, freeze-dried bacterial agent and apple pomace protein feed for fermenting apple pomace

A technology for fermenting apple pomace and compound bacterial agent, applied in animal feed, animal feed, microorganism-based methods, etc., can solve the problems of pollution, sour odor, waste of resources and environment, etc., and achieves convenient storage and high viable bacteria rate. , the effect of increasing protein content

Inactive Publication Date: 2020-10-09
GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] But at present, most of the apple pomace in our country is discarded because it cannot be comprehensively utilized. Under the joint action of the microorganisms in the apple pomace and the microorganisms in the environment, it will rot and deteriorate within a few days, giving off a sour smell, resulting in a serious waste of resources. and environmental pollution

Method used

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  • Composite bacterial agent, freeze-dried bacterial agent and apple pomace protein feed for fermenting apple pomace
  • Composite bacterial agent, freeze-dried bacterial agent and apple pomace protein feed for fermenting apple pomace
  • Composite bacterial agent, freeze-dried bacterial agent and apple pomace protein feed for fermenting apple pomace

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preparation example Construction

[0029] In the present invention, the preparation method of the seed liquid preferably includes: inoculating the freeze-dried microbial agent described in the above technical scheme in the wort liquid medium for the first culture to obtain the first culture; Inoculate in apple pomace medium for second culture to obtain seed solution.

[0030] In the present invention, the wort liquid culture medium is preferably filled with ddH 2 O is a solvent, and each liter includes 120-140 g of wort medium, more preferably 130.1 g of wort medium. The present invention has no special limitation on the preparation method of the wort liquid culture medium, preferably the wort culture medium and ddH 2 Prepared by dissolving O and sterilizing at 121°C for 15 minutes. In the present invention, there is no special limitation on the source of the wort culture medium, and conventional commercially available products can be used.

[0031] In the present invention, the temperature of the first cult...

Embodiment 1

[0039] Screening of compound bacterial strains for fermenting apple pomace:

[0040] 1.1 Strain activation

[0041]After sterilizing the ampoules of separately preserved freeze-dried strains Geotrichum candidum, Saccharomyces, Rhizopus, Bacillus subtilis, Trichoderma viride, Aspergillus niger and Aspergillus oryzae, open the ampoules respectively, add culture medium to dissolve them, and transplant them into corresponding On the slant culture medium where each bacterial species grows, culture at 31°C for 24h-72h. The results showed that Geotrichum candidum, Saccharomyces, Rhizopus, Bacillus subtilis, Trichoderma viride, Aspergillus niger and Aspergillus oryzae could all grow well on the slant medium. The slant medium inoculated with Geotrichum candidum, yeast, Rhizopus, Bacillus subtilis, Trichoderma viride, Aspergillus niger and Aspergillus oryzae are the medium for routine cultivation of the above-mentioned bacteria.

[0042] 1.2 Strains grow on different contents of apple...

Embodiment 2

[0063] The inoculum amount of apple pomace fermented by compound bacterial agent

[0064] Inoculate Geotrichum candidum + Yeast + Rhizopus + Bacillus subtilis + Trichoderma viride on the wort liquid medium according to the inoculation ratio of 2:2:0.5:0.5:1, and cultivate them for 16 hours as basic seeds; The seeds were inoculated in 100 g of dry apple pomace medium (the ratio of material to water was 10:8), and cultured at 31°C for 48 hours as primary seeds; the primary seeds were inoculated in sterilized fresh apple pomace in different proportions ( The dry matter content of apple pomace is 20%, the moisture content is 80%) in 100g of culture medium, after cultivating at 31°C for 72h, the fermented apple pomace is dried at 65°C; The product is the control group. The protein content of apple pomace before and after fermentation was determined by biuret method. The results are shown in Table 3.

[0065] Table 3 Effects of different inoculum amounts of compound bacterial age...

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Abstract

The present invention provides a composite micro-organism agent and a freeze-dried micro-organism agent for fermenting apple pomace, and an apple pomace protein feed, and relates to the technical field of animal fermented feeds. The composite micro-organism agent for fermenting the apple pomace comprises the following components in parts by volume: 29-36 parts of geotrichum candidum, 16-36 parts of yeasts, 8-31 parts of bacillus subtilis, 8-17 parts of rhizopus and 8-17 parts of trichoderma viride; and effective viable micro-organism number of the geotrichum candidum, yeasts, bacillus subtilis, rhizopus and trichoderma viride are respectively independently larger than or equal to 1x10<9> cfu / ml. The composite micro-organism agent can compensate defects for each other during fermentation and is subjected to synergistic mutual fermentation. The apple pomace protein feed produced by the provided composite micro-organism agent has a crude protein content of 20% or more, the total of 16 amino acids is more than 7.54 g / 100 g, and the composite micro-organism agent is low in fiber, comprehensive in nutrition, green, safe, low in cost and high in benefits.

Description

technical field [0001] The invention relates to the technical field of animal fermented feed, in particular to a composite bacterial agent for fermenting apple pomace, a freeze-dried bacterial agent and apple pomace protein feed. Background technique [0002] my country is the largest producer of apple planting area and annual output in the world, accounting for 55% of the world's total output. At present, in addition to selling fresh apples to domestic and foreign markets, the production of apple juice has become one of the main products in the extension of the apple industry chain and one of the main ways of deep processing of apples. [0003] Apple pomace is a by-product of juicing apples. According to reports, my country's apple processing is used for juicing every year, and more than one million tons of pomace are produced annually. With the rapid development of fruit juice processing industry, the output of pomace is also increasing. Apple pomace is a by-product pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14A23K10/12A23K10/37A23K20/105A23K20/20A23K20/24A23K20/26C12R1/125C12R1/845C12R1/885C12R1/645
CPCA23K10/12A23K10/37A23K20/105A23K20/20A23K20/24A23K20/26C12N1/14C12N1/16C12N1/20Y02P60/87
Inventor 陈伯祥郭慧琳赵子惠杨明成伟伟李元新仉连平
Owner GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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