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Method for producing lipopeptide biosurfactant through fermentation

A biosurfactant and active agent technology, applied in the field of fermentation, can solve the problems of high separation cost, immature industrial production, low yield of biosurfactant, etc., and achieve the effect of improving economic benefits, fermentation yield and fermentation efficiency

Active Publication Date: 2015-12-02
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The yield of biosurfactant produced by the existing fermentation method is low, the cost of separation is high, and the industrial production is immature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This example illustrates Bacillus subtilis Bacillussubtilis G-34 Process steps for producing surfactin biosurfactant in shake flasks. among them Bacillussubtilis G-34 was isolated by our laboratory and deposited in the China Type Culture Collection on January 6, 2014, with the deposit registration number CCTCCNO: M2014003.

[0034] The specific training steps are as follows:

[0035] (1) Preparation of seed culture medium: 5g / L yeast extract powder, 10g / L peptone, 10g / L sodium chloride; sterilize at 121°C for 20 minutes, cool for later use.

[0036] (2) Preparation of fermentation medium: glycerol 20g / L, disodium hydrogen phosphate 10g / L, dipotassium hydrogen phosphate 3g / L, ammonium nitrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.02 g / L, use phosphoric acid (1mol / L) to adjust the pH to 8.0. Sterilize at 115°C for 30 minutes. Cool down for use.

[0037] (3) Seed culture: Inoculate a loop of Bacillus subtilis in the medium obtained in step (...

Embodiment 2-8

[0041] This example illustrates the influence of different immobilized cell carriers on the production of surfactin.

[0042] (1) Cultivation method The method used in Example 1 was used for cultivation.

[0043] (2) In the process of preparing the fermentation medium, for every 50ml of fermentation medium, add 2.5g / L of activated carbon, agar, expanded clay, attapulgite, towel, gauze and sodium alginate to immobilize it in Example 2-8. Carrier.

[0044] (3) In Examples 2-8, the control samples taken are the samples in Example 1.

[0045] (4) After the fermentation, the concentration of lipopeptide biosurfactant is measured.

[0046] The measurement results are shown in Table 1:

[0047] Table 1

[0048] Example number2345678 Surfactin concentration (mg / L)83057668087012001034367

Embodiment 9

[0050] This example illustrates the steps of the method for obtaining surfactin in a 5L stirred fermenter.

[0051] (1) Seed culture medium preparation: yeast extract powder 5g / L; peptone 10g / L; sodium chloride 10g / L. Sterilize at 121°C for 20 minutes, and cool down for later use.

[0052] (2) Preparation of fermentation medium: glycerol 20g / L, potassium dihydrogen phosphate 1g / L, ammonium nitrate 2g / L, magnesium sulfate heptahydrate 0.2g / L, ferrous sulfate heptahydrate 0.02g / L; sterilized at 115°C 30min. Cool down for use.

[0053] (3) Modification of the 5L stirred fermentation tank. At the exhaust port, install a foam separation column with an inner diameter of 2cm and a height of 25cm. At the same time, use a towel as a fixed material to fix it on the built-in baffle of the fermentation tank. The size of the towel is 15×44cm. .

[0054] (4) Seed culture: inoculate a loop of Bacillus subtilis in the medium obtained in step (1), culture at 37°C with a shaker rotation speed of 200...

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PUM

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Abstract

The invention discloses a method for producing lipopeptide biosurfactant through fermentation. Bacillus subtilis is immobilized on a solid material and a dual-phase pH-controlled continuous batch fermentation technology is employed, so surfactant production efficiency is effectively improved.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and relates to a method for producing lipopeptide biosurfactants by immobilized Bacillus subtilis, in particular to a two-stage pH control method for producing lipopeptide biosurfactants by continuous fermentation using the strain. Background technique [0002] Lipopeptides are a class of compounds composed of hydrophilic peptide chains and lipophilic aliphatic hydrocarbon chains, which contain peptide chains composed of 7-10 amino acids and β-hydroxy fatty acid chains or β-amino fatty acid chains. The hydroxyl group or amine group combines with the hydroxyl group on the amino acid of the peptide chain to form a lactone bond or an amide bond, which closes the peptide chain to form a cyclic lipopeptide. Due to the special chemical composition and amphiphilic molecular structure of lipopeptide, it has broad application prospects in the fields of medicine, food, agriculture and microbial oil r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12R1/125
Inventor 李霜黄和刘强徐晴江凌
Owner NANJING UNIV OF TECH
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