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Method for producing long-chain dicarboxylic acid by regulating and controlling fermentation process through redox potential

A long-chain dibasic acid and potential regulation technology, applied in the field of fermentation engineering, can solve the problems of large oxygen demand, large oxygen demand and large heat release

Active Publication Date: 2016-07-13
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Large oxygen demand and heat release: when microorganisms ferment n-alkanes to generate dibasic acids, four oxygen atoms are added, so the oxygen demand is large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In the present embodiment, the production of long-chain dibasic acid is carried out by the following method, which specifically includes the following steps:

[0048] (1) Strain activation: take a piece of Candidaviswanathii (Candidaviswanathii) and spread it on a large test tube of 20×180mm on a solid slant medium, and cultivate it at 27°C for 72h. The slant medium is malt with pH=6.4 juice agar medium;

[0049] (2) Seed culture: inoculate the activated bacterial classification on the seed culture medium (100mL) in the 500ml Erlenmeyer flask, at 27 ℃, shake culture 40h under 200r / min, the seed culture medium used in the described seed culture process The composition is glucose 20g / L, disodium hydrogen phosphate 2g / L, yeast extract 1g / L, corn steep liquor 2g / L, urea 0.5g / L, n-dodecane 100mL / L, and the pH of the seed medium is 6.0;

[0050] (3) acid production by fermentation: the seed culture obtained in step (2) was inoculated into a 7.5L fermenter with 3L fermentatio...

Embodiment 2

[0054] The only difference between this example and Example 1 is that the fermentation acid production process is: the seed culture obtained in step (2) is inoculated into a 7.5L fermenter with 3L fermentation medium at an inoculum size of 10% (parallel Carry out three experiments), control the initial pH 4.0, control the fermentation temperature at 27°C, the ventilation rate of sterile air is 0.1vvm, the stirring speed is 50rpm, when the ORP is lower than 0mV, gradually increase the stirring speed (maximum no more than 1000rpm) , Ventilation (maximum not exceeding 4.0vvm) and adding reducing agent or oxidizing agent, control the ORP near 0mV, adjust the pH to 7.0, 8.0 and 9.0 when the glucose is consumed, and control the ORP near 50mV, at the same time through continuous or intermittent Add n-dodecane in a way so that the concentration of n-dodecane in the fermentation broth is always greater than or equal to 1% (v / v), cultivate for 114 hours, and the acid production is 40.6g / ...

Embodiment 3

[0058] The only difference between this example and Example 1 is that the fermentation acid production process is: the seed culture obtained in step (2) is inoculated into a 7.5L fermenter with 3L fermentation medium at an inoculum size of 10% (parallel Carry out three experiments), control the initial pH 4.0, control the fermentation temperature at 27°C, the ventilation rate of sterile air is 0.1vvm, the stirring speed is 50rpm, when the ORP is lower than 0mV, gradually increase the stirring speed (maximum no more than 1000rpm) , ventilation (maximum not exceeding 4.0vvm) and adding reducing agent or oxidizing agent, control the ORP around 0mV, adjust the pH to 7.0, 8.0 and 9.0 when the glucose is consumed, and control the ORP around 100mV, and at the same time through continuous or intermittent Add n-dodecane in a way so that the concentration of n-dodecane in the fermentation broth is always greater than or equal to 1% (v / v), cultivate for 114 hours, and the acid production ...

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Abstract

The invention provides a method for producing long-chain dicarboxylic acid by regulating and controlling the fermentation process through redox potential.The method comprises the steps of strain activating, seed culture and fermentation acid production.A fermentation system is a four-phase fermentation system formed by thalluses, sterile air, alkane and water, an ORP electrode is introduced into the fermentation system, and two-stage OPR control fermentation is achieved to produce the long-chain dicarboxylic acid by regulating the ventilatory capacity and stirring rotating speed in combination with an oxidizing agent and a reducing agent.According to the method, the ORP electrode is introduced in the process of producing the long-chain dicarboxylic acid through microbial fermentation, so that a two-stage ORP control strategy is achieved; the fermentation process of the long-chain dicarboxylic acid is regulated, and therefore the yield of the long-chain dicarboxylic acid and the stability of the fermentation system are improved; the fermentation yield is remarkably improved compared with a method that ORP is not controlled, even the fermentation yield is 4.6 times that of the method that the ORP is not controlled, and the production intensity can reach 1.78 g.h-1.L-1.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to a method for producing long-chain dibasic acids by using redox potential to regulate the fermentation process. Background technique [0002] Long-chain dibasic acids are important raw materials for the synthesis of fragrances, engineering plastics, hot-melt adhesives, coatings, lubricating oils, resins and medicines. At present, long-chain dibasic acids are mainly produced by fermenting n-alkanes by using the special function of microbial double-end oxidation, but the production efficiency of this process is very low. For example, the production intensity of mixed long-chain dibasic acids is only 0.92g h -1 ·L -1 (CN101225411), the production intensity of seventeen carbon dibasic acid is only 1.1g h -1 ·L -1 (CN102115769) and dodecane dibasic acid production intensity is only 1.3g h -1 ·L -1 (CN1928100). In order to improve the production efficiency of long-c...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/72
CPCC12P7/6409
Inventor 万印华曹伟锋陈向荣杭晓风
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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