Process for preparing biological antibacterial agent

A biological antibacterial agent and seed technology, applied in the field of bioengineering, can solve the problems of ecological environment system damage, chemical control of fungal diseases and other problems, achieve the effects of crop and environmental safety, increase the yield of antipeptide, and simple nutritional requirements

Inactive Publication Date: 2005-11-23
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, chemically synthesized pesticide products are usually used for control. Although they have certain effects, their long-term use in large quantities has caused great damage to the ecological environment system, and due to the diversity, variability and drug resistance of pathogenic bacteria, It also brings difficulties to the chemical control of fungal diseases

Method used

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  • Process for preparing biological antibacterial agent
  • Process for preparing biological antibacterial agent
  • Process for preparing biological antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Use 10 1000mL Erlenmeyer flasks, each with 300mL medium A (glucose 2.0% peptone 3.0% beef extract 0.5% magnesium sulfate 0.1% potassium dihydrogen phosphate 0.2%), sterilize at 120°C, cool and inoculate with activated Jie’an The copeptin-producing bacterium Bacillus firmus bacterium liquid was placed at a temperature of 30° C. and shaken for 18 hours. Inoculate the cultivated seed liquid into 50L sterile medium B (1.0% starch, 2.0% dextrin, 1.0% lactose, 2.0% sucrose, 0.5% glycerin, 1.0% corn flour, waste molasses, 3.0% glucose, etc.) 2.0% peptone 5.0% ammonium nitrate 1.0% ammonium sulfate 0.5% peanut cake powder 2.0% soybean cake powder 0.5% biological nitrogen 1.0% potassium dihydrogen phosphate 0.5% zinc sulfate 0.5% sodium chloride 0.5% iron sulfate 0.1%.) In a 100L fermenter, at a temperature of 28°C-30°C, after 8-15 hours of aerated and stirred fermentation, medium B is continuously fed at a rate of 0.1-1.0L / h until about 12 hours before the fermentation is stopp...

Embodiment 2

[0054] Use 100 1000mL Erlenmeyer flasks, each bottle filled with 300mL medium A (glucose 3.0%, peptone 2.0%, beef extract 0.8%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.5%.) Sterilize at 120°C, cool and inoculate the activated Jiean The copeptin-producing bacterium Bacillus firmus bacterium liquid was placed at a temperature of 30° C. and shaken for 18 hours. Inoculate the cultivated seed liquid into 250L sterile medium B (2.0% starch, 2.0% dextrin, 1.0% lactose, 1.0% sucrose, 0.5% glycerol, 0.5% corn flour, 3.0% glucose 1.0% peptone 1.0% ammonium nitrate 3.0% ammonium sulfate 2.0% peanut cake powder 5.0% soybean cake powder 2.0% biological nitrogen 3.0% potassium dihydrogen phosphate 0.1% zinc sulfate 0.1% sodium chloride 0.5% iron sulfate 0.5%.) In a 500L fermenter, at a temperature of 28°C-30°C, after fermentation with aeration and stirring for 10-15 hours, add medium B continuously at a rate of 0.5-2.0L / h until about 12 hours before stopping fermentation (l...

Embodiment 3

[0057] Embodiment 3: use 100 of 1000mL Erlenmeyer flasks, every bottle packs 300mL medium A (glucose 4.0% peptone 3.0% beef extract 0.8% 8 magnesium sulfate 0.3% potassium dihydrogen phosphate 0.4%.) Sterilize at 120 ℃, inoculate after cooling The activated Jieanpeptide-producing bacterium Bacillus firmus was cultured at 30°C for 18 hours in a shaker flask. Inoculate the cultivated seed solution into 250L sterile medium B (starch 2.0%, dextrin 1.0%, lactose 1.0%, sucrose 2.0%, glycerin 0.5%, corn flour 0.5, waste molasses 0.5%, glucose 3.0) according to the inoculation amount of 10%-15%. % peptone 5.0%, ammonium nitrate 0.5%, ammonium sulfate 0.5%, peanut cake powder 1.0%, soybean cake powder 2.0%, biological nitrogen 5.0%, potassium dihydrogen phosphate 1.0%, zinc sulfate 0.1%, sodium chloride 0.1%, iron sulfate 0.5%.) 500L In the fermenter, at a temperature of 28°C-30°C, after aeration and stirring for 10-15 hours, medium B is intermittently fed, and the intermittent time is...

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Abstract

The invention belongs to the field of biological engineering and is aimed for solving the problem of mass scale plant diseases caused by fungus. The preparing process comprises subjecting a strong of Bacillus firmus (CGMCC No.0924) to second level fermentation culture under suitable condition, then carrying out filtering, salting out and column chromatography treatment.

Description

[0001] 1. Field [0002] The invention belongs to the field of bioengineering, and in particular relates to a method for preparing active substances by fermentation of microorganisms. 2. Background technology [0003] Plant diseases often bring heavy losses to agricultural production and are one of the main reasons for the reduction of world food production. However, 80% of plant diseases are caused by plant pathogenic fungi. How to effectively prevent and treat plant fungal diseases has always been one of the problems to be solved in agricultural production. At home and abroad, chemically synthesized pesticide products are usually used for control. Although they have certain effects, their long-term use in large quantities has caused great damage to the ecological environment system, and due to the diversity, variability and drug resistance of pathogenic bacteria, It also brings difficulties to the chemical control of fungal di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04
Inventor 谭红周金燕雷宝良李志东杨杰钟娟白冰如
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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