Artificial method for inducing natural spawning and incubation of xenopus laevis

Abstract

The invention relates to an artificial method for inducing natural spawning and incubation of xenopus laevis. The method comprises the following steps: selecting sexually mature female xenopus laevis which do not lay eggs within 3 months and sexually mature male xenopus laevis which do not discharge sperms within 3 months; subcutaneously injecting hormone in the female xenopus laevis and male xenopus laevis; placing the xenopus laevis into a spawning pond according to the method of two male and one female or three male and two female or four male and two female; and after the female xenopus laevis lay eggs, taking out the oosperms and placing the oosperms into an incubator to incubate, wherein the oosperms are changed into little tadpoles after incubating for 24 to 72 hours. The method promotes natural spawning of the xenopus laevis, is simple to operate, convenient to popularize, low in cost and low in technical difficulty, fills the gap of quick spawning of the xenopus laevis in China, can provide sufficient tadpoles for mass breeding, and is applicable to test in the scientific research institutions and commercial breeding of the xenopus laevis.

Description

technical field [0001] The invention relates to an artificial method for inducing natural oviposition and hatching of Xenopus laevis. Background technique [0002] The African clawed frog (Xenopus laevis) is an amphibian that is widely used in scientific research. At present, there is no large-scale breeding in our country, and scientific research experiments need to be imported from abroad, and there is no large-scale breeding. The main reason is that it is difficult to lay eggs and hatch them. It is difficult to provide tadpoles in time to lay eggs once a year under natural conditions. At present, some scientific research institutes adopt the method of artificial fertilization: inject a certain amount of hormone to the female frog to make the female frog remove eggs, dissect the male frog, take out the testicle of the male frog, and carry out artificial fertilization in a specific nutrient solution. This method not only wastes resources, but also has high technical diffic...

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This method not only wastes resources, but als

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