Medicine composition for preventing acute schistosome infection

A composition and technology of schistosomiasis, applied in the field of medicine, can solve the problems of ineffective worm eggs and poor curative effect, and achieve good insecticidal effect, prevention and treatment of percutaneous infection, and good curative effect

Active Publication Date: 2016-06-15
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, at present, most of the prevention and treatment of acute schistosomiasis is the oral treatment of praziquantel, but it has the problem of poor curative effect. It has a good effect of killing adults, but it is invalid for eggs. Better drugs for schistosomiasis

Method used

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  • Medicine composition for preventing acute schistosome infection
  • Medicine composition for preventing acute schistosome infection
  • Medicine composition for preventing acute schistosome infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Effect of the pharmaceutical composition of the present invention on liver morphology in mice with acute Schistosoma japonicum infection.

[0033] Experimental method: get 35 6-8 week old BALB / c male mice and group by random number table, be respectively: normal control group, praziquantel control group (500mg / kg), medicine low dose group of the present invention (dye lignin 25 mg / kg+praziquantel 500 mg / kg), the drug high dose group of the present invention (genistein 75 mg / kg+praziquantel 500 mg / kg). Except for the mice in the normal control group, the mice in the other groups were infected with 18±2 cercariae by the abdominal application method, and at the fifth week after the infection of Schistosoma japonicum, the normal group was given normal saline, and the rest of the groups were given 500 mg / kg praquine After two days of ketone, the corresponding dose of genistein was administered, and the mice were treated at the sixth week, and the liver was collecte...

Embodiment 2

[0034] Example 2 Effect of the pharmaceutical composition of the present invention on granuloma formation caused by acute Schistosoma japonicum infection.

[0035] Experimental method: the pretreatment is the same as in Example 1. Liver tissue from the same part of the liver was fixed with 4% paraformaldehyde, dehydrated and transparent (dehydrated with 70% ethanol, 80% ethanol, 90% ethanol, 95% ethanol for 3 hours each, dehydrated with absolute ethanol Ⅰ and absolute ethanol Ⅱ for each 2h, xylene I and xylene II transparent for 20 minutes each, paraffin I and paraffin II soaked in wax for 40 minutes each), embedding and sectioning, the section thickness is 4 μm, oven-dried the sections at 50°C, carried out HE staining, and sealed the slides. Sealed with permanent gum and observed pathologically.

Embodiment 3

[0036] Example 3 Immunohistochemical observation of the effect of the pharmaceutical composition of the present invention on the expression of P65 protein in liver tissue of mice with acute Schistosoma japonicum infection.

[0037] Paraffin sections were dewaxed to water; 3% H 2 o 2 Incubate at room temperature for 5-10min, wash with distilled water; soak in PBS for 5min; block with 5-10% normal goat serum (diluted in PBS), incubate at room temperature for 10min; Appropriately diluted biotin-labeled secondary antibody (1% BSA-PBS dilution), incubate at 37°C for 10-30min; wash with PBS, 5min×3 times; drop appropriate diluted horseradish enzyme-labeled streptavidin; 37°C Incubate for 10-30min; rinse with PBS, 5min×3 times; develop color with chromogen (DAB); fully rinse with tap water, counterstain, and mount the slide.

[0038] Experimental method: the pretreatment is the same as in Example 1.

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Abstract

The invention discloses a medicine composition for preventing acute schistosome infection and belongs to the technical field of medicines. The medicine composition is composed of genistein and praziquantel according to the weight ratio of 25-75:500. The medicine composition, compared with the praziquantel, has better treatment effects. The medicine composition can recover normal liver tissue shape, eliminate granuloma, greatly reduce inflammatory cells and ensure normal P65 protein expression. The medicine composition has excellent insecticide effects on schistosoma japonicum and can be used for preventing and treating acute schistosoma japonicum per-cutaneous infection.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a pharmaceutical composition for preventing and treating acute schistosomiasis infection. Background technique [0002] Schistosoma japonicum is also called schistosome; platyhelminth, fluke class, polymorpha, schizotyke. There are three kinds of Schistosoma japonicum (Schistosome japonicum), Schistosoma haematobium and Schistosoma mansoni that parasitize the human body; Schistosoma parasitizes the portal vein system in humans and animals, which can cause schistosomiasis. [0003] Schistosoma haematobium parasitizes most vertebrates, and 10 species parasitize humans: Schistosomahaematobium (Schistosoma haematobium, that is, Schistosoma haematobium) lives in the bladder vein and is mainly distributed in Africa, southern Europe and the Middle East. The eggs pass through the wall of the vein into the bladder, where they are excreted with urine. Larvae develop in intermediate host...

Claims

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Application Information

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IPC IPC(8): A61K31/4985A61P33/12A61K31/352
CPCY02A50/30
Inventor 万春鹏姚亮亮黄卫锋陈金印
Owner JIANGXI AGRICULTURAL UNIVERSITY
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