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SiRNA of SjLGL gene of schistosoma japonica and use thereof

A technology for schistosomiasis and schistosomiasis, applied in the fields of molecular biology and biomedicine, can solve the problems of "large first-pass effect, affecting clinical application effect, and poor bioavailability.

Inactive Publication Date: 2011-10-19
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, drugs, such as praziquantel, are mainly used to treat people or animals infected with schistosomiasis, but when praziquantel is taken orally, the "first-pass effect" is large and the bioavailability is poor, which affects the clinical application effect

Method used

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  • SiRNA of SjLGL gene of schistosoma japonica and use thereof
  • SiRNA of SjLGL gene of schistosoma japonica and use thereof
  • SiRNA of SjLGL gene of schistosoma japonica and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 RNA interference in vitro

[0028] 1. Method steps

[0029] 1.1 Preparation of juvenile worm culture medium

[0030]

[0031] Dissolve in RPMI-1640, dilute to 1000ml, filter with a filter with a pore size of 0.22μm, and store at 4°C.

[0032] 1.2 Preparation of rabbit red blood cells

[0033] (1) Add a little autoclaved PBS solution containing 0.1% heparin sodium into a sterile 1.5ml centrifuge tube and shake it several times to coat the tube wall;

[0034] (2) 1ml of blood was collected from the ear vein of New Zealand white rabbits, and quickly transferred into a centrifuge tube containing heparin; gently shake the centrifuge tube to mix the anticoagulant;

[0035] (3) Centrifuge at 1500rpm for 10min at 4°C, discard the supernatant;

[0036] (4) Add 1ml sterilized PBS and a small amount of double antibody to the tube, and gently blow and mix with a micropipette;

[0037] (5) Centrifuge at 1500rpm for 10min at 4°C, discard the supernatant;

[0038] (...

Embodiment 2

[0062] Example 2 RNA interference in vivo

[0063] 1. Method steps

[0064] (1) dsRNA injection

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Abstract

The invention discloses the siRNA of SjLGL gene of schistosoma japonica. The siRNA may be one pair or any more than two pairs of nucleotide sequences represented by SEQ ID No.1 and SEQ ID No.2, nucleotide sequences represented by SEQ ID No.3 and SEQ ID No.4, and nucleotide sequences represented by SEQ ID No.5 and SEQ ID No.6. The invention also discloses the use of the siRNA of the SjLGL gene of schistosoma japonica. The siRNA of the SjLGL gene of schistosoma japonica can obviously inhibit the transcription of the SjLGL gene, obviously lower the hatchability of the eggs of insects in livers of mice infected with schistosomes and is suitable for preparing medicines for treating schistosomiasis.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and biomedicine, in particular to an siRNA of the SjLGL gene of Schistosoma japonicum and its application. Background technique [0002] Schistosomiasis is a parasitic disease that can infect both humans and animals. It has a wide range of epidemics and causes serious harm to both humans and livestock. At present, drugs, such as praziquantel, are mainly used to treat people or animals infected with schistosomiasis. However, when praziquantel is taken orally, the "first-pass effect" is large and the bioavailability is poor, which affects the clinical application effect. It is necessary to find new drugs for the treatment of schistosomiasis. [0003] Larval giant lethal gene (Lethal Giant Larvael, LGL) refers to a gene that can cause excessive cell proliferation and tissue hyperplasia when mutated. Mutations in the LGL gene can lead to excessive proliferation of Drosophila epithelial ce...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61P33/12
Inventor 金亚美石艳丽刘萍萍杨云霞刘金明林矫矫
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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