Fluorescent quantitative PCR reagent kit for fast detecting Japanese blood fluke

Abstract

The invention discloses a fluorescence quantitative PCR reagent box for rapid detection of Schistosoma japonicum, relates to gene detection technology of a kind of pathogen of zoonotic infectious disease, suitable for qualitative and quantitative detection of Schistosoma japonicum. The invention comprises of DNA extracting solution, fluorescence quantitative PCR reaction solution, standard positive template PU-SJ and negative quality control standard sample; the fluorescence quantitative PCR reaction solution contains specific primers and fluorescence probe of Schistosoma japonicum. The invention was accurate in quantitative detection, quick with good specificity and high sensitivity; can identify and detect Schistosoma japonicum and Chinese schistosomiasis; the steps are simple with good repeatability; can qualitatively and quantitatively detect the DNA samples of all three species of Schistosoma japonicum group, can replace traditional aetiology methods, oncomelania crushing method of detecting the snail of schistosome cercariae and larva escape method.

Description

technical field [0001] The invention relates to a gene detection technology for zoonotic infectious disease pathogens, in particular to a fluorescent quantitative PCR (polymerase chain reaction) kit for rapid detection of Schistosoma japonicum, which is suitable for qualitative and quantitative detection of Schistosoma japonicum. Background technique [0002] Schistosoma is also called Schistosoma. In 1851, the German pathologist Theodor Bilharz first discovered Schistosoma haematobium adult worms when he performed an autopsy on a hematuria patient in a Cairo hospital. So far, 19 species of Schistosoma have been found. Six of the 19 species of schistosomes parasitize humans: Schistosoma japonicum, Schistosoma mansoni, Schistosoma haematobium, Schistosoma intercalatum, Schistosoma mekongi and Schistosoma malayi (Wu Zhengjian, Chinese Medical Encyclopedia of Parasitology and Parasitology〔M ], Shanghai Science and Technology Press, 1984.). The 19 species of schistosomes of th...

AI Technical Summary

Problems solved by technology

[0006] In terms of clinical detection, traditional etiological methods and serological methods have disadvantages such as low sensitivity, poor specificity, false positives, time-consuming and labor-intensive, etc.; although conventional PCR method has the advantages of simplicity, rapidity and sensitivity, it cannot be accurately quantified and post-PCR Deal with problems such as false positives caused by pollution; therefore, it is necessary to develop an accurate, sensitive, fast, and pollution-free clinical testing method