Ginkgoic acid preparation method using macroporous adsorption resin

Abstract

The invention provides a ginkgoic acid preparation method using macroporous adsorption resin, comprising the following steps: absorbing ginkgoic acid from the feed liquid containing ginkgoic acid until being saturated using the macroporous adsorption resin; using water to wash out the residual feed liquid which is not absorbed by the resin; washing the impurities with lower resin absorption compared with the ginkgoic acid using the non-alkaline hydrophilic organic solvent aqueous solution; eluting ginkgoic acid constituents using the non-acidity hydrophilic organic solvent aqueous solution; adjusting the p H value of the eluent; decompression recovering the solvent and vacuum concentrating and drying the eluent to produce the finished products of the ginkgoic acid. The preparation method has features of novel design, simple and easily executed technology, best quality, low production cost, and can be used in further separation and purification of the feed liquid containing ginkgoic acid from separate sources with larger generalization.

Description

technical field [0001] The invention relates to a method for separating and purifying natural products, more specifically to a method for preparing natural product ginkgolic acid by using a macroporous adsorption resin. Background technique [0002] Ginkgo biloba is a rare and precious tree species unique to China. It is known as the "living fossil of the plant kingdom". It is listed as a national second-class protected plant and is included in the first batch of Chinese herbal medicines with the same origin of medicine and food. China's ginkgo resources account for the world's total 70% of. [0003] Both ginkgo fruit and ginkgo leaves have high medicinal value and edible value. Modern pharmacological research shows that its active ingredients mainly include flavonoids, lactones, ginkgolic acid and polyprenol. Vascular disease, senile peripheral circulation disorder, senile dementia have good curative effect, and ginkgolic acid and polyprenol have obvious anti-tumor, anti-o...

AI Technical Summary

Problems solved by technology

But, the technology that this adopts lipophilic organic solvent extraction, silica gel column chromatography purification or HPLC preparative chromatogram to prepare ginkgolic acid by methods such as multiple separations has the disadvantages that the permeability of lipophilic solvent is not strong, the consumption of solvent and silica gel is large, and the solvent Disadvantages of high toxicity, high preparation cost, large investment in equipment, and difficult implementation of industrialized safe production

[0011] There are also scholars in China who improve the deficiencies of the above-mentioned process, and use 80% industrial ethanol to extract ginkgolic acid instead, then use macroporous adsorption resin to refine, and ethanol gradients of different concentrations elute ginkgolic acid [Ni Xuewen et al. Research on acid, Chinese herbal medicine, 2003, 34(3): 231-233; Ni Xuewen et al., Extraction method of ginkgolic acid from Ginkgo testa and its antitumor activity in vitro, Jiangsu Agricultural Science, 2006(5): 151-153] , but because ginkgolic acid is a fat-soluble compound, it is difficult to dissolve in polar solvents such as water, methanol, ethanol, and the technology of using this non-alkaline ethanol aqueous solution to extract and elute ginkgolic acid still has solvent permeability is not strong, solvent Disadvantages of large consumption, high solvent toxicity, high preparation cost, low yield and purity of ginkgolic acid

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