Technique for removing ginkgolic acid in folium ginkgo extract

A technology of gingkgolic acid and ginkgo biloba, which is applied in the field of ginkgolic acid removal technology, can solve the problems of high removal cost, low removal rate, and content reduction, and achieve the effect of low cost and simple process

Active Publication Date: 2008-06-11
石药银湖制药有限公司
View PDF0 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most domestic manufacturers in my country use solvent extraction or single resin adsorption. Some of the above methods have low removal rates, and the ginkgophenolic acid in the Ginkgo biloba extract is less than 5PPM; some have complex extraction processes and high removal costs; While removing ginkgolic acid, the content of total flavonoids and total lactones in ginkgo also decreased

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The present invention uses the ginkgo biloba extract passed through the macroporous resin as a raw material, adopts the combined method of polyamide and strong basic macroporous ion exchange resin, and removes ginkgolic acid. The process includes the following steps:

[0015] 1. Weigh 2 g of Ginkgo biloba extract that has passed through the macroporous resin, and dissolve it with ultrapure water;

[0016] 2. Add the pretreated polyamide into the solution, stir for 8 hours, and filter out the polyamide after static sedimentation;

[0017] 3. Pass the filtrate of (2) through D918 strong basic macroporous ion exchange resin, wash with water until colorless, and then wash with buffer solution until clear;

[0018] 4. Recover the eluent and spin dry in vacuum to obtain a product with a ginkgolic acid content of less than 5 ppm.

Embodiment 2

[0020] 1. Dissolve 2 g of the weighed Ginkgo biloba extract in 75% ethanol;

[0021] 2. Add the pretreated polyamide remover to the solution of (1), fully stir for 8 hours, then filter out the polyamide after static sedimentation;

[0022] 3. Pass the filtrate of (2) through a D918 strongly basic macroporous ion exchange resin column, wash with an alkaline buffer solution (NH3 H2O-NH4CL, Ph=9.5) until colorless, recover the eluent, and obtain ginkgolic acid Extracts with a content of less than 5ppm.

[0023] Determination of Ginkgo biloba extract and Ginkgo biloba extract Ginkgophenolic acid content after removal treatment by HPLC method, and calculate the removal rate according to the following formula:

[0024] Ginkgolic acid removal rate=(1-the ginkgolic acid content in the ginkgo leaf extract after processing ÷ the ginkgolic acid content in the original ginkgo leaf extract) * 100%, the results are shown in Table 1:

[0025] Table 1 Content of ginkgolic acid in ginkgo bil...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a removal technique of ginkgolic acid in a ginkgo leaf extract, which utilizes ginkgo leaf extract which passes through a macroreticular resinous to be raw material, and the process comprises firstly utilizing polyamide which is pre-processed to be a removing agent of the ginkgolic acid, and then changing superpositions of resin pillar with pre-processing strong alkalinity macroporous ion to remove ginkgolic acid. The process has simple technique, stability and low cost, which can effectively remove ginkgolic acid in ginkgo leaf extract, and can not affect yield rates of ginkgo biloba flavonoids and ginkgolide.

Description

Technical field: [0001] The invention relates to a chemical process, in particular to a process for removing ginkgolic acid from ginkgo leaf extract. Background technique: [0002] Ginkgo biloba total flavonoids and ginkgo lactones contained in Ginkgo biloba extract have unique effects on the treatment of cardiovascular and cerebrovascular diseases, cerebral ischemia, brain aging and asthma. However, Ginkgo biloba extract also contains sensitizing toxic substances - ginkgolic acid and ginkgolic acid, which are used as drugs for the treatment of cardiovascular and cerebrovascular diseases. International standards require that the ginkgolic acids in Ginkgo biloba extract must be reduced to below 5PPM . However, in the current commercially available ginkgo leaf extract products, the content of ginkgolic acid is above 500PPM, and some even reach 2000PPM, which seriously affects the safety of the drug and cannot be exported. There are more than 100 ginkgo leaf extract factories ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P9/00B01D15/08
Inventor 苏小建辛云海何星存陈全斌李俊
Owner 石药银湖制药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products