Application of ginkgoic acid and ginkgol compounds to killing Ampullaria gigas

A technology of ginkgophenolic acid and compounds, applied in the field of biopesticides, can solve the problems of easy harm to the ecological environment, difficulty and cost of prevention and control, and unsuitable for large-scale use.

Inactive Publication Date: 2010-12-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, although there are many ways to control apple snails, such as: winter rice field trimming, scientific water management, manual removal of egg masses, release of ducks to eat snails, etc., due to factors such as the stability of the effect, the choice of implementation timing, the acquisition of materials, and the cost, etc. restrictions, most of them are not suitable for large-scale use
T

Method used

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  • Application of ginkgoic acid and ginkgol compounds to killing Ampullaria gigas
  • Application of ginkgoic acid and ginkgol compounds to killing Ampullaria gigas
  • Application of ginkgoic acid and ginkgol compounds to killing Ampullaria gigas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: The effect of ginkgolic acid homologues on killing apple snails

[0021] Preparation of ginkgolic acid homologues Weigh 100 g of dry ginkgo biloba testa and crush it, ultrasonically extract 5 times the amount of petroleum ether three times, each time for 2 hours, combine the extracts, filter under reduced pressure, evaporate the solvent to obtain petroleum ether extract. Dissolve the extract in a small amount of petroleum ether and apply it to a silica gel column (Φ3.2×40cm), elute with petroleum ether: ether: formic acid = 89:11:1 (v / v / v), and perform thin-layer chromatography Fluorescence at 254nm was detected, and the ginkgolic acid fraction was collected; the column was repeated several times. Finally, the ginkgolic acid components were washed with water until neutral, and the eluent was evaporated to obtain a homologous mixture of ginkgolic acids.

[0022] The HPLC analysis Varian high performance liquid chromatograph of ginkgolic acid homologue, Sin...

Embodiment 2

[0027] Example 2: The effect of ginkgolic acid C13:0 monomer on killing apple snails

[0028] Preparation of ginkgolic acid C13:0 Dissolve ginkgolic acid homologues in tetrahydrofuran: methanol = 1:1, and separate ginkgolic acid monomers with HPLC semi-preparative column HiQ C18 (250×21.2mm, 15 μm), mobile phase is methanol: 3% Acetic acid=90:10, UV detector detects the absorption peak at 280nm, collects the first peak, repeats once, and obtains ginkgolic acid C13:0 monomer. The structural formula is as follows:

[0029]

[0030] The HPLC analysis of ginkgolic acid C13:0 Varian high-performance liquid chromatograph, SinoChrom ODS-AP C18 (250 * 4.6mm, 5 μ m) analysis column, mobile phase is methanol: 3% acetic acid=92: 8 (v / v), flow rate 1.0mL / min, the ultraviolet detection wavelength is 310nm, the isolated ginkgolic acid C13:0 is analyzed, and compared with the standard, the purity of the detected ginkgolic acid C13:0 reaches 86%.

[0031] Ginkgolic acid C13:0 killing app...

Embodiment 3

[0036] Example 3: The effect of ginkgolic acid C15:1 monomer on killing apple snails

[0037] Preparation of Ginkgolic Acid C15:1 Dissolve ginkgolic acid homologues in tetrahydrofuran:methanol=1:1, separate ginkgolic acid monomer with HPLC semi-preparative column HiQ C18 (21.2×250mm, 15μm), mobile phase is methanol:3% acetic acid =90:10, flow velocity 3.0mL / min, UV detector detects absorption peak at 280nm place, collects the second peak, repeats once, obtains ginkgolic acid C15:1 monomer. The structural formula is as follows:

[0038]

[0039] The HPLC analysis of ginkgolic acid C15:1 Varian high-performance liquid chromatograph, SinoChrom ODS-AP C18 (250mm * 4.6mm, 5 μ m) analytical column, mobile phase is methanol: 3% acetic acid=92: 8 (v / v), flow velocity 1.0mL / min, the ultraviolet detection wavelength is 310nm, and the separated ginkgolic acid C15:1 is analyzed, and the purity of the detected ginkgolic acid C15:1 reaches 80%.

[0040] Ginkgolic acid C15:1 killing app...

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Abstract

The invention belongs to the technical field of biopesticides, discloses application of ginkgoic acid and ginkgol compounds to killing agriculturally harmful mollusks, and discloses novel application of the ginkgoic acid and ginkgol to preparation of the biopesticides. The invention shows that the ginkgoic acid and ginkgol compounds can effectively inhibit the synthesis of glycogen and protein inmollusk ampullaria gigas, can simultaneously inhibit the activities of ChE, SDH and MDH in the cephalopodium and the liver of the ampullaria gigas, interfere a NADH respiratory chain and cause energymetabolic dysfunction so as to cause cell energy supply deficiency and unattainability of normal synthesis and secretion functions, and promote the lack of important proteins and enzymes in the body of the ampullaria gigas so as to cause the death of the ampullaria gigas. The ginkgoic acid and the ginkgol are readily degradable in the natural environment, and have small influence on the environment; and an acute toxicity test proves that the ginkgoic acid and the ginkgol are proved to be slightly toxic pesticides, can be developed into novel biopesticides, and can be used for preventing the ampullaria gigas for rice, vegetables and fruits.

Description

technical field [0001] The invention relates to biopesticides, in particular to the new application of ginkgolic acids and ginkgols (ginkgols, also known as ginkgool) in the preparation of biopesticides, in particular to the use of ginkgolic acids and gingkools to kill apple snails, an agricultural pest. Background technique [0002] Apple snail [Pomacea canaliculata (Lamarck)] is also called big bottle snail, apple snail, snow snail in my country, and it is a kind of aquatic snail. Originated in the Amazon River Basin in South America, it was introduced into Taiwan as an economic animal in the late 1970s. It was introduced into Guangdong in 1981, and then introduced into Guangxi, Fujian, Hainan, Zhejiang, Shanghai, Jiangsu and other places. They don't like to eat, and the economic benefits of breeding are poor. They were soon abandoned in the farmland environment and gradually developed into harmful organisms for crops. It was one of the first 16 alien species that invaded ...

Claims

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Application Information

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IPC IPC(8): A01N37/40A01N31/08A01P9/00
Inventor 杨小明任晓锋刘伟民
Owner JIANGSU UNIV
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