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Method for culturing polygonum cuspidatum seedlings

A cultivation method and technology of Polygonum cuspidatum, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of large area, long growth cycle, difficulty in realizing large-scale industrial planting and cultivation, etc., and achieve low environmental pollution, The effect of increased growth rate and improved planting efficiency

Inactive Publication Date: 2015-09-09
SHENZHEN XINHUA NANFANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the planting and cultivation of Polygonum cuspidatum mainly adopts the traditional nursery method for seedling cultivation, which has a long growth cycle and a large area, making it difficult to achieve efficient and rapid large-scale industrial planting and cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of Polygonum cuspidatum seedling cultivating method of the present invention comprises the steps:

[0029] The first step is to carry out aseptic bud culture: take the tender stems or shoots of Polygonum cuspidatum as explants, first disinfect them with alcohol, then wash them once with sterile water, and then use (0.05% to 0.15%) HgCl 2 After sterilization, wash with sterile water for 3-5 times, and then connect it to a sterile bud culture medium for cultivation, and obtain sterile buds after about 12 to 18 days.

[0030] The concentration of disinfectant alcohol can be 70%-80%, for example, 75% alcohol has a good disinfection effect.

[0031] The number of days of cultivation is mainly determined by the growth rate of the buds of Polygonum cuspidatum. Generally, it is advisable for the sterile buds to grow to about 1 cm. Of course, if the sterile sprout is longer than 1 centimeter (for example, grows to 1.5 centimeters) or shorter than 1 centimeter (for exampl...

Embodiment 2

[0063] This embodiment is basically the same as Embodiment 1, the difference is:

[0064] In step one, "carry out aseptic bud culture: take the tender stem or tender shoot of Polygonum cuspidatum as explant, first disinfect it with alcohol, then wash it once with sterile water, and then use (0.05% ~ 0.15%) HgCl 2 After sterilization, wash with sterile water for 3-5 times, then inoculate on sterile bud medium for cultivation, and obtain sterile buds after about 12 to 18 days.”

[0065] Or put the Polygonum cuspidatum with tender buds into a biochemical incubator, and after cultivating at 38-40°C for 6-8 days, cut the tender stems or shoots of Polygonum cuspidatum and disinfect with alcohol and (0.05%-0.15%) HgCl 2 After sterilization, after cleaning with sterile water for 3-5 times, cut out about 2mm long bud tips and put them into sterile bud culture medium. After the buds grow, cut out about 2mm long bud tips and put them into sterile bud culture basal culture, so repeated t...

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Abstract

The invention provides a method for cultivating polygonum cuspidatum seedlings, which comprises the steps of cultivating aseptic seedlings, cultivating multiple shoots, cultivating strong seedlings, rooting, acclimatization and transplant. During the step of cultivating aseptic seedlings, a polygonum cuspidatum tender stem / shoot is adopted as an explant. The polygonum cuspidatum tender stem / shoot is sterilized by alcohol, disinfected by 0.05-0.15% of HgCl2, rinsed by sterile water, inoculated in a 1 / 2 MS or an MS with a moderate amount of plant growth hormone, and cultivated for 12-18 days to obtain aseptic seedlings. During the step process of cultivating multiple shoots, an already grown aseptic seedling is cut off, inoculated in a multiple shoot culture medium prepared through adding a moderate amount of plant growth hormone in a 1 / 2 MS, and cultivated. During the step of strong seedling cultivating and rooting, when multiple shoots are grown to about 1 cm in size, the multiple shoots are inoculated into strong seedling culture media and rooting culture media, grouped in bottles and cultivated, where in the culture media are pared by 1 / 2 MS and / or MS with a moderate amount of plant growth hormone. The above steps are conducted at the cultivating temperature of 15-30 DEG C and under the illumination intensity of 2000-3000 LUX, wherein the illumination time is 6-12 hours / day. During the step of acclimatization and transplant, regenerated plants from root cultures are transplanted in a matrix composed of garden soil and peat in the ratio of 2:1, shaded by a shading net, and grow out of the matrix after being cultivated at the humidity of 60-70% for 15-30 days. According to the invention, the growth speed and the plantation benifit of polygonum cuspidatum seedlings are greatly improved. meanwhile, the method is low in cost, small in pollution, simple in process, applicable in all seasons and suitable for large-scale culture of seedlings.

Description

technical field [0001] The invention relates to a method for cultivating Polygonum cuspidatum seedlings. Background technique [0002] Polygonum cuspidatum is a medicinal plant with extremely high value. Using Polygonum cuspidatum as raw material can extract a variety of high value-added medicinal, beauty and health care raw materials. For example, polydatin, an extract from Polygonum cuspidatum, can be used for antitussive, blood lipid regulation, lowering cholesterol, anti-shock, etc. Polydatin injection with polydatin as the main component is the first Chinese traditional medicine class 1 innovative drug submitted for clinical application in the United States; Polygonum cuspidatum extract resveratrol and its glycosides have inhibitory effect on cell and tissue variation in the process of carcinogenesis. Resveratrol has been listed as one of the most promising anti-cardiovascular and anti-cancer drugs. It can lower blood fat, Anti-virus, anti-bacteria, anti-oxidation, and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 覃国乐何寻阳韦英明韩苗苗吴峰
Owner SHENZHEN XINHUA NANFANG BIOTECH
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