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Polygonum cuspidatum root endophytic strain G3 for efficiently transforming polydatin into resveratrol

A technology of knotweed root and microbial strains, applied in the direction of microorganisms, fungi, and methods based on microorganisms, which can solve the problems of low conversion rate, labor, environmental pollution, etc.

Inactive Publication Date: 2011-09-28
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods have low specificity, cumbersome operation, high cost, low conversion rate (1.23%-1.68%), and cause pollution to the environment
However, it is time-consuming, labor-intensive, and wasteful to extract active ingredients from natural resources by chemical methods. Therefore, microbial transformation methods are gradually applied to the research of natural products.
But so far there is no report about the transformation of Polygonum cuspidatum by using plant endophytes or human intestinal bacteria

Method used

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  • Polygonum cuspidatum root endophytic strain G3 for efficiently transforming polydatin into resveratrol
  • Polygonum cuspidatum root endophytic strain G3 for efficiently transforming polydatin into resveratrol
  • Polygonum cuspidatum root endophytic strain G3 for efficiently transforming polydatin into resveratrol

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Experimental program
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Effect test

Embodiment 1

[0026] The roots of wild Polygonum cuspidatum collected from Tianxinping Village, Lianyuan, Hunan Province were washed with sterile water, and then blotted dry with sterile filter paper. (Following aseptic operation) Soak in 75% ethanol for 3 to 5 minutes, rinse with sterile water for 3 to 4 times, disinfect with 0.1% mercuric chloride for 30 seconds, and then sterilize with 75% ethanol for 1 minute. Cut off the phloem of the roots and stems, and cut the xylem into small pieces of 0.5 cm × 0.5 cm with sterile scissors. Rinse with sterile water 3 to 4 times, and grind into a slurry in a sterilized mortar. After diluting the grinding slurry 10 times with sterile water, pipette 1ml of the grinding diluent, and routinely spread it on the primary screening medium. Each material was cultured in parallel 3 times at 28°C for 72-120 hours, and well-growing colonies of different shapes were selected and separated on a PDA plate. After 72 hours of cultivation at 28°C, a single colony wa...

Embodiment 2

[0028] Adopt HPLC method to detect the content of polydatin and resveratrol in the fermented liquid:

[0029] Preparation of reference solution and drawing of standard curve

[0030] Accurately weigh 0.2 mg of standard resveratrol and polydatin in 1 mL of methanol, mix well to obtain a stock solution containing 0.2 mg / mL of resveratrol and polydatin reference substance, and set aside. Precisely draw 100, 200, 300, 400, 500 μL of the reference substance solution and dilute it into 1 mL with methanol, and mix well to make reference substances of different concentrations. Determine the peak area integral value according to the chromatographic conditions, take the peak area Y as the ordinate, and the injection concentration X as the abscissa, draw a standard curve, see Figure 5 and Figure 6 , and calculate the regression equation.

[0031] Sample solution preparation

[0032] Insert bacterial strain G3 of the present invention in 10% Polygonum cuspidatum decoction (pH nature...

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Abstract

The invention discloses a Polygonum cuspidatum root endophytic strain. The strain is named Penicillium oxalicum G3 in a classified way, the strain was collected in China General Microbiological Culture Collection Center on January 17, 2011, and the collection number is CGMCC NO.4558. Polydatin in Polygonum cuspidatum can be transformed into resveratrol by inoculating the strain into a culture medium containing Polygonum cuspidatum boiling extracting solution, so that the polydatin can be directly transformed into the resveratrol through microbial fermentation, the extraction of the polydatin is not required, the cost can be saved, and the pollution to the environment can be reduced.

Description

Technical field: [0001] The invention relates to an endophytic bacterium of Polygonum cuspidatum, in particular to endophytic bacterium G3 of Polygonum cuspidatum root which can convert polydatin into resveratrol under co-fermentation condition with Polygonum cuspidatum. Background technique: [0002] Resveratrol in Polygonum cuspidatum is a natural active polyphenol substance, which has the functions of inhibiting tumors, anti-oxidation, and anti-free radicals. It has been listed as one of the most promising anti-cardiovascular and anti-cancer drugs. Another green anti-cancer substance in the future. Resveratrol in Polygonum cuspidatum often exists in the form of polydatin, and the content of resveratrol in dried Polygonum cuspidatum is only 0.1%-0.2%, while the content of polydatin is about 2%, and polydatin is decomposed into Resveratrol exerts its pharmacological effects. [0003] There are many domestic reports on increasing the content of resveratrol by transforming ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/22C12R1/80
Inventor 易有金刘华金夏菠
Owner HUNAN AGRICULTURAL UNIV
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