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A strain of Inonotus obliquus qd04 and method for transforming Polygonum cuspidatum into resveratrol, triterpene saponins and polysaccharides

A technology of Inonotus obliquus, QD04, which is applied in the fields of biotransformation and fermentation engineering of natural products, can solve problems such as uncontrollable conditions, pollution, and unknown flora, and achieve reduction of resource waste and environmental pollution, high nutritional value, and additional high value effect

Active Publication Date: 2017-12-22
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the flora in the natural fermentation process is unknown, the conditions are difficult to control, and the mold in the air may produce toxic substances; the microorganisms used in the special microbial transformation method are not disclosed, cannot be verified and repeated, and its practicability is greatly limited
Furthermore, the reported microbial strains that efficiently transform resveratrol glycosides are mainly molds, which not only may have potential safety hazards in the obtained resveratrol products, but also produce a large amount of mycelium and dregs during the fermentation process, causing resource pollution. Waste and Environmental Pollution

Method used

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  • A strain of Inonotus obliquus qd04 and method for transforming Polygonum cuspidatum into resveratrol, triterpene saponins and polysaccharides
  • A strain of Inonotus obliquus qd04 and method for transforming Polygonum cuspidatum into resveratrol, triterpene saponins and polysaccharides
  • A strain of Inonotus obliquus qd04 and method for transforming Polygonum cuspidatum into resveratrol, triterpene saponins and polysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The screening method of Inonotus obliquus QD04 provided by the invention is:

[0035] 1) Screening of cellulase-secreting strains:

[0036] Thirteen kinds of medicinal fungi white rot fungi (collected and preserved by the Shandong Key Laboratory of Applied Fungi, Qingdao Agricultural University) were inoculated on cellulose-Congo red medium plates, cultured for 144 hours, and the diameter of the transparent circle (D) and the diameter of the colony were measured (d), in cm, see Table 1. The results showed that the D / d value of strains QD01, 02, 04, 06, 07 and 10 was greater than 1, which preliminarily judged that they might have strong ability to synthesize cellulase.

[0037] Table 1 Screening results of cellulase-producing strains on cellulose-Congo red plates

[0038] Strain number

144 hours transparent ring diameter

144 hours colony diameter

144 hours ratio of transparent circle to colony diameter

QD01

3.00

1.20

2.50

QD0...

Embodiment 2

[0045] Embodiment 2: Inonotus obliquus QD04 shake flask fermentation Polygonum cuspidatum

[0046] 1) Seed culture:

[0047] The formula of the seed medium is: according to the mass volume percentage, glucose 2%, peptone 0.5%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O 0.15%, bran 0.5%, the balance is water, the liquid volume in a 250ml Erlenmeyer bottle is 50ml, and sterilized at 121°C for 20min.

[0048] Seed culture was carried out in a 250ml Erlenmeyer flask with a liquid volume of 50mL, a shaker rotation speed of 150rpm, a culture temperature of 28°C, and a culture time of 96 hours;

[0049] 2) Fermentation culture

[0050] Fermentation medium: add 3g bran and 100ml tap water to boil for 20min, filter, add water to the filtrate to 100ml, take 50ml and put it into a 250ml Erlenmeyer flask, add 2g knotweed powder (60 mesh), 1g sucrose, 0.25g peptone, KH 2 PO 4 0.15g, and MgSO 4 ·7H 2 O 0.075g, sterilized at 121°C for 20min.

[0051] The carbon source, nitrogen source and in...

Embodiment 3

[0054] Embodiment 3: Inonotus obliquus QD04 batch fermentation Polygonum cuspidatum

[0055] 1) Seed culture:

[0056] Seed culture medium and seed culture process are with embodiment 1.

[0057] 2) Fermentation culture

[0058] Fermentation medium: 35g bran and 1000ml tap water, boil for 20min, filter, add water to the filtrate to 1000ml, add Polygonum cuspidatum powder 60g / L (60 mesh), sucrose 10g / L, peptone 4g / L, KH 2 PO 4 2g / L, and MgSO 4 ·7H 2 O2g / L.

[0059] The fermentation culture uses a 5 L fermenter with a liquid volume of 3 L, an inoculum size of 10% (v / v), a fermentation temperature of 28 °C, a rotation speed of 300 rpm, an air volume of 1 vvm, and 5 mol / L NaOH Control the pH value to 6.0. The method of batch fermentation is adopted, and the fermentation is 8 days.

[0060] Experimental results: the conversion rate of resveratrol in the fermentation broth was 99.85%, the yield of triterpenoid saponins was 898.15±4.23μg / mL, and the yield of polysaccharides ...

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Abstract

The invention provides a strain of Inonotus obliquus QD04 and a method for transforming Polygonum cuspidatum to produce resveratrol, triterpene saponins and polysaccharides. The Inonotus obliquus QD04 is preserved in the China Type Culture Collection Center, numbered CCTCC M 2015036 , the present invention uses the medicinal fungus QD04 to convert resveratrol glycosides in plants into resveratrol, which greatly improves the yield of resveratrol, with simple process and good repeatability; the bacteria can use Polygonum cuspidatum to synthesize its own The active substances are triterpene saponins and polysaccharides; the fermentation product Inonotus obliquus mycelium is rich in essential amino acids and has high nutritional value; resveratrol, triterpene saponins, polysaccharides and obliquus obliquus can be obtained at the same time in one fermentation process Four kinds of mycelia products have high added value in the production process; the Inonotus obliquus can more thoroughly hydrolyze the cellulose of Polygonum cuspidatum, fully utilize the biomass resources of Polygonum cuspidatum, and reduce waste of resources and environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of biotransformation and fermentation engineering of natural products, and specifically relates to a strain of Inonotus obliquus QD04 and a method for transforming Polygonum cuspidatum into resveratrol, triterpene saponins and polysaccharides. Background technique [0002] Resveratrol (3,4',5-trihydroxy-trans-stilbene) is a non-flavonoid polyphenol compound, which exists in more than 70 kinds of plants such as knotweed, grapes, peanuts, etc. A phytoalexin produced by Studies have shown that resveratrol has the effects of anti-cancer, anti-oxidation, protection of cardiovascular system, scavenging free radicals and delaying aging. [0003] The production methods of resveratrol include chemical synthesis and extraction from natural plants. The chemical synthesis method is costly and pollutes the environment, so the current production of resveratrol is mainly extracted from plants. However, the content of re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/22C12P19/04C12P33/20C12R1/645
CPCC12N1/14C12P7/22C12P19/04C12P33/20C12N1/145C12R2001/645
Inventor 刘琳郭立忠
Owner QINGDAO AGRI UNIV
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