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Method for preparing root systems containing resveratrol with culturing of polygonum cuspidatum leaves

A technology of resveratrol and Polygonum cuspidatum, applied in the field of cultivating roots containing resveratrol by using Polygonum cuspidatum leaves, can solve problems such as inconsistent consumption concepts, achieve stable and controllable output and quality, reduce manpower and material resources consumption, and facilitate operation Effect

Active Publication Date: 2016-07-20
YICHUN UNIVERSITY
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Problems solved by technology

And because the hairy root is produced by the integration of a section of T-DNA of the rooting plasmid of Agrobacterium rhizogenes into the nucleus genome of the plant explant, the hairy root obtained by this method is a transgenic product , which is inconsistent with the current consumption concept of people pursuing pure natural non-GMO

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  • Method for preparing root systems containing resveratrol with culturing of polygonum cuspidatum leaves
  • Method for preparing root systems containing resveratrol with culturing of polygonum cuspidatum leaves
  • Method for preparing root systems containing resveratrol with culturing of polygonum cuspidatum leaves

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Embodiment 1

[0034] (1), prepare aseptic Polygonum cuspidatum seedlings,

[0035] A. Disinfection of explants: cut wild knotweed young stems with joints, and disinfect them first, that is, wash them with running water first, soak them in 75% alcohol for 15 seconds in an ultra-clean working environment, and then use 0.1% mercuric chloride Soak for 4-5min, then rinse 5 times with sterile water, dry the water with sterile filter paper and then use the explants for inoculation;

[0036] B. Rapid propagation of explants: inoculate the explants after disinfection in the previous step on the differentiation rapid propagation medium: MS+6-BA2.0mg / L+IBA0.2mg / L+sucrose 30g / L+agar 7g / L , the pH value is 5.8, and the medium is sterilized at 121°C for 15-20 minutes; the light conditions are: light intensity 3000lx, light time 14h / d, culture temperature: 25°C±1°C;

[0037] C. Strong seedling cultivation: inoculate the Polygonum cuspidatum seedlings obtained through differentiation and rapid propagation...

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Abstract

The invention relates to a method for preparing root systems containing resveratrol with culturing of polygonum cuspidatum leaves. Adventitious roots and hairy roots are directly obtained through the polygonum cuspidatum leaves and callus tissues thereof, then the adventitious roots and the hairy roots are used as raw materials, and the resveratrol is extracted from the raw materials. The method comprises the following steps: (1) preparing bacteria-free polygonum cuspidatum seedlings; (2) preparing different explant callus tissues of polygonum cuspidatum; (3) performing direct differentiation and rooting on the callus tissues of the polygonum cuspidatum so as to obtain polygonum cuspidatum root systems. Therefore, a new way for replacing wild polygonum cuspidatum resources is provided.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and relates to a method for directly obtaining a large amount of root systems by culturing leaves of Polygonum cuspidatum, in particular to a method for preparing root systems containing resveratrol by culturing leaves of Polygonum cuspidatum. Background technique: [0002] Knotweed (Polygonumcuspidatum) is a plant of the Polygonaceae family, and resveratrol is its main active ingredient. Resveratrol (resveratrol) is a non-flavonoid polyphenol compound with various biological activities. It is a kind of phytoalexin produced by plants under external stimuli. The root of Polygonum cuspidatum has the highest content. Resveratrol can not only improve the disease resistance of plants, but also has a variety of pharmacological effects beneficial to human health, mainly including: anti-oxidation, anti-tumor, anti-inflammatory and anti-bacterial, anti-aging, prevention of cardiovascular disease, estrogen, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 卢其能肖林霞李润根
Owner YICHUN UNIVERSITY
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