Polygonum cuspidatum stem endophytic fungus J1 capable of converting polydatin to resveratrol

A technology of Polygonum cuspidatum and microbial strains, applied in microorganisms, fungi, microorganism-based methods, etc., can solve the problems of cumbersome operation, low specificity, and time-consuming active ingredients.

Inactive Publication Date: 2011-10-12
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods have low specificity, cumbersome operation, high cost, low conversion rate (1.23%-1.68%), and cause pollution to the environment
However, it is time-consuming, labor-intensive, and wasteful to extract active ingredients from natural resources by chemical methods. Therefore, microbial transformation methods are gradually applied to the research of natural products.
But so far there is no report about the transformation of Polygonum cuspidatum by using plant endophytes or human intestinal bacteria

Method used

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  • Polygonum cuspidatum stem endophytic fungus J1 capable of converting polydatin to resveratrol
  • Polygonum cuspidatum stem endophytic fungus J1 capable of converting polydatin to resveratrol
  • Polygonum cuspidatum stem endophytic fungus J1 capable of converting polydatin to resveratrol

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Experimental program
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Effect test

Embodiment 1

[0022] The stems of wild Polygonum cuspidatum collected from Tianxinping Village, Lianyuan, Hunan Province were washed with sterile water, and then dried with sterile filter paper. (Following aseptic operation) Soak in 75% ethanol for 3 to 5 minutes, rinse with sterile water for 3 to 4 times, disinfect with 0.1% mercuric chloride for 30 seconds, and then sterilize with 75% ethanol for 1 minute. Cut off the phloem of the roots and stems, and cut the xylem into small pieces of 0.5 cm × 0.5 cm with sterile scissors. Rinse with sterile water 3 to 4 times, and grind into a slurry in a sterilized mortar. After diluting the grinding slurry 10 times with sterile water, pipette 1ml of the grinding diluent, and routinely spread it on the primary screening medium. Each material was cultured in parallel 3 times at 28°C for 72-120 hours, and well-growing colonies of different shapes were selected and separated on a PDA plate. After 72 hours of cultivation at 28°C, a single colony was take...

Embodiment 2

[0024] Adopt HPLC method to detect the content of polydatin and resveratrol in the fermented liquid:

[0025] Preparation of reference solution and drawing of standard curve

[0026] Accurately weigh 0.2 mg of standard resveratrol and polydatin in 1 mL of methanol, mix well to obtain a stock solution containing 0.2 mg / mL of resveratrol and polydatin reference substance, and set aside. Precisely draw 100, 200, 300, 400, 500 μL of the reference substance solution and dilute it into 1 mL with methanol, and mix well to make reference substances of different concentrations. Determine the peak area integral value according to the chromatographic conditions, take the peak area Y as the ordinate, and the injection concentration X as the abscissa, draw a standard curve, see Figure 5 and Image 6 , and calculate the regression equation.

[0027] Sample solution preparation

[0028] Insert bacterial strain J1 of the present invention in 10% Polygonum cuspidatum decoction (pH nature)...

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Abstract

The invention relates to a polygonum cuspidatum stem endophytic fungus of which the category name is penicillium oxalicum, wherein the endophytic fungus is conserved in the China General Microbiological Culture Collection Center on January 17, 2011 and the CGMCC NO is 4559. The endophytic fungus is inoculated in the culture medium of polygonum cuspidatum extract so that polydatin in polygonum cuspidatum can be converted to resveratrol. Therefore, polydatin can be directly converted to resveratrol through microbial fermentation, the extraction of polydatin is not required, the cost can be saved and environmental pollution can be reduced.

Description

Technical field: [0001] The invention relates to an endophytic bacterium of Polygonum cuspidatum, especially an endophytic bacterium J1 of Polygonum cuspidatum stem which can convert polydatin in Polygonum cuspidatum into resveratrol under the co-fermentation condition with Polygonum cuspidatum. Background technique: [0002] Resveratrol in Polygonum cuspidatum is a natural active polyphenol substance, which has the functions of inhibiting tumors, anti-oxidation, and anti-free radicals. It has been listed as one of the most promising anti-cardiovascular and anti-cancer drugs. Another green anti-cancer substance in the future. Resveratrol in Polygonum cuspidatum often exists in the form of polydatin, and the content of resveratrol in dried Polygonum cuspidatum is only 0.1%-0.2%, while the content of polydatin is about 2%, and polydatin is decomposed into Resveratrol exerts its pharmacological effects. [0003] There are many domestic reports on increasing the content of res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/22C12R1/80
Inventor 易有金刘华金夏菠
Owner HUNAN AGRICULTURAL UNIV
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