Penicillium vulpinum fungi strain and method for preparing levo 7-hydroxyl butylphthalide employing fungi strain
A technology of hydroxybutylphthalide and penicillium, applied in the field of microorganisms, can solve the problems of low content of L-7-hydroxybutylphthalide, difficulty in industrial application, low product yield, etc., and achieve easy separation and purification, suitable for industrial production , the effect of a single fermentation component
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0046] Example 1, the isolation and identification of Penicillium vulpeides NCC3421
[0047] (a) Isolation of strain NCC3421
[0048] The medium used for the isolation of fungal strain NCC3421 is PDA (Beijing Shuangxuan);
[0049] Take 1 gram of soil sample (collected from the mountainous soil in Baoxing County, Ya'an City, Sichuan Province, China), put it into a 50 mL centrifuge tube filled with 9 mL of sterile water, and shake fully. Take 1mL of the suspension and put it into a test tube filled with 9mL of sterile water, and mix well. Serial serial dilutions were carried out until 10 -10 . Take 0.2 mL of each gradient to plate. The coated plate was cultured statically at 26°C, and a fungus was obtained from it, which was named NCC3421.
[0050] (b) Identification of strain NCC3421—morphological characteristics and molecular identification
[0051] The spore liquid of strain NCC3421 was inoculated in PDA medium, cultured at 25°C for 7 days, and observed under scanning e...
Embodiment 2、5
[0065] The preparation of embodiment 2,5L tank fermentation 7-hydroxybutylphthalide
[0066] (a) Preparation of the seed solution of Penicillium voxenum strain NCC3421
[0067] The Penicillium voxenum strain NCC3421 was inoculated into the seed medium, and cultured at 26 °C and 200 rpm for 72 h to obtain the seed solution. The preparation method of the seed medium is: 20.0 grams of corn starch, 10.0 grams of glucose, 2.0 grams of hot-pressed soybean cake powder, 6.0 grams of malt powder, 3.0 grams of yeast powder, 2.0 grams of sodium chloride, 1.0 grams of magnesium sulfate heptahydrate, and tap water Dissolve and dilute to 1000 ml, pH 6.8, and sterilize at 121 °C for 30 min.
[0068] (b) Preparation of the fermentation broth of Penicillium vulpeides strain NCC3421
[0069] The seed solution was inoculated into a fermenter with 5 L of fermentation medium at an inoculum volume of 5% by volume. The tank temperature was 26 °C, the tank pressure was 0.05±0.01 Mpa, the ventilatio...
Embodiment 3
[0090] Embodiment 3, the preparation of shake flask fermentation 7-hydroxybutylphthalide
[0091] (a) Preparation of seed solution of Penicillium vulpecum strain NCC3421
[0092] The Penicillium voxenum strain NCC3421 was inoculated into the seed medium, and cultured at 26 °C and 200 rpm for 72 h to obtain the seed solution. The preparation method of the seed medium is: 20.0 grams of corn starch, 10.0 grams of glucose, 2.0 grams of hot-pressed soybean cake powder, 6.0 grams of malt powder, 3.0 grams of yeast powder, 2.0 grams of sodium chloride, 1.0 grams of magnesium sulfate heptahydrate, and tap water Dissolve and dilute to 1000 ml, pH 6.5, and sterilize at 121 °C for 30 min.
[0093] (b) Preparation of fermentation broth of Penicillium vulpeides strain NCC3421
[0094] The seed solution was inoculated into a 250 mL shake flask containing 40 mL of fermentation medium at an inoculum volume of 5% by volume, and fermented for 144 h at 26 °C with a rotation speed of 200 rpm. ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com