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Preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism

A technology of Penicillium oxalicum and Penicillium oxalicum, which is applied in the field of environmental biology to achieve the effect of reducing infection, promoting rot and good application prospects

Active Publication Date: 2021-01-01
随县山水农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the use of Penicillium oxalicum spores to control wheat head blight in rice-wheat rotation areas

Method used

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  • Preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism
  • Preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism
  • Preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Screening of bacterial strains of the present invention

[0039] Weighed 10 g of rhizosphere soil samples from rice-wheat rotation field in Phosphate Mine Township, Zhongxiang, Hubei, and put them into conical flasks filled with 90 mL of PKO medium, and cultivated them at 28°C and 150 rpm for 2 days. Take 0.2 mL of the soil suspension after enrichment culture, spread it on the inorganic phosphorus solid medium, and incubate it upside down at 28°C for 7 days, and purify the strains with phosphorus-dissolving circles. Inoculate the fungus obtained from the preliminary screening on a PDA plate, and after the colony is full, take a piece of fungus block with a 0.7 cm diameter hole puncher and inoculate it into a conical flask containing 200 mL of PKO medium. Shake culture. The supernatant was taken every day to measure pH and available phosphorus content (molybdenum antimony colorimetric method), and the measurement was continuous for 8 days. Through the abov...

Embodiment 2

[0040] Morphological observation and PCR identification of embodiment 2 bacterial strains

[0041] The strain MEM02 was inoculated on a PDA plate and cultured upside down at 28°C for 7 days. The colony of the strain MEM02 was blue-gray, and the opposite side was light yellow, producing a large number of conidia with neat edges, opaque, and no exudate. Wash the bacterial strain MEM02 plate with sterile water to obtain Penicillium oxalicum MEM02 spores, microscopically observe as follows: figure 2 , spore shape is oval. The high-efficiency phosphate-dissolving fungus MEM02 was inoculated on the YPD plate, and cultured upside down at 28°C for 3-4 days until a large number of hyphae were produced. The fungal universal primer ITS1 (5 ， -TCCGTAGGTGAACCTGCGG-3 ， ) and ITS4 (5 ， -TCCTCCGCTTATTGATATGC-3 ， )

[0042] Amplify.

[0043] PCR reaction amplification system is 50 μL: upstream and downstream primers (10 μmol / L) ITS1 and ITS4 each 1 μL, DNA template 2 μL, Easy Taq DNA P...

Embodiment 3

[0045] Example 3 Preparation of Penicillium oxalicum MEM02 high-yield spore-producing solid fermentation agent

[0046] Use a hole punch with a diameter of 0.7 cm to inoculate the Penicillium oxalicum block on the PDA solid medium from the Penicillium oxalicum MEM02 plate for activation for 3 days, and rinse with 10 mL of sterile water to obtain the Penicillium oxalicum spore suspension. Take the activated spore suspension and evenly inoculate it into the MEM02 high-efficiency spore-producing solid fermentation medium designed by this patent according to the inoculation amount of 5% (5% of the mass of the fermentation medium) and ferment for 5 days at 28°C, and mix the Erlenmeyer flask every day once. The obtained solid fermentation product is Penicillium oxalicum MEM02 solid fermentation to produce high-concentration spore preparation. After counting on a hemocytometer, the concentration of Penicillium strawberry spores reached 2.29×10 9 CFU / g.

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Abstract

The invention discloses a preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism, and belongs to the technical field of environmental biology. The penicillium oxalicum MEM02 can inhibit germination of fusarium graminearum spores in soil and remarkably reduce infection of the fusarium graminearum spores to wheat, and meanwhile the penicillium oxalicum MEM02 can efficiently solubilize phosphorus and promote plant growth. Therefore, the penicillium oxalicum MEM02 has good application prospects in the aspect of developing new biopesticides or new biological control microbial agents.

Description

technical field [0001] The invention belongs to the field of environmental biology technology, and in particular relates to a preparation method of Penicillium oxalicum strain MEM02 and an application thereof. The Penicillium oxalicum can efficiently decompose phosphorus, promote plant growth, and prevent and control wheat scab. Background technique [0002] Phosphorus is one of the three major mineral nutrients necessary for plant growth. Phosphorus fertilizer is an important material guarantee for increasing grain production and food security. Improving the utilization efficiency of soil phosphorus and phosphorus fertilizer is an important measure for saving phosphorus fertilizer and sustainable agricultural development. About 74% of the cultivated land in my country is deficient in phosphorus, and most of the phosphorus is immobilized phosphorus that is difficult for plants to absorb and utilize. In agricultural production, the application of phosphorus fertilizers is ofte...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01N63/36A01P3/00A01P21/00C12R1/80
CPCA01N63/36C12N1/14C12N3/00C12N1/145C12R2001/80
Inventor 倪红许银孙孝文王行国李亚东姚伦广杨立军
Owner 随县山水农业发展有限公司
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