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Geobacillus thermodenitrificans TB62 and application thereof in promoting compost maturity

A geobacillus and denitrification technology is applied in the field of plant growth-promoting bacteria development to achieve the effects of accelerating DOM degradation, fast growth and reproduction, and increasing compost temperature

Active Publication Date: 2017-08-18
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the Bacillus strains used mainly include Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Bacillus toyoi, etc. There are few reports on the research on bacilli, such as the invention patent of application number 201510455215.7 "A Strain of Thermal Denitrifying Geobacillus CF-1 and Its Use", which discloses that the preservation number is CGMCC No.10697, and discloses that the strain is Uses in Microbial Oil Recovery

Method used

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  • Geobacillus thermodenitrificans TB62 and application thereof in promoting compost maturity
  • Geobacillus thermodenitrificans TB62 and application thereof in promoting compost maturity
  • Geobacillus thermodenitrificans TB62 and application thereof in promoting compost maturity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 : Screening of Geobacillus thermodenitrificans TB62 (Geobacillus thermodenitrificans)

[0041] 1. Experimental materials and equipment

[0042] 1.1 Main reagents

[0043] Table 1 Main Reagents

[0044] Drug Name molecular formula Specification ABTS C 18 h 24 N 6 o 6 S 4

AR hydrogen peroxide h 2 o 2

AR citric acid C 6 h 8 o 7

AR Sodium citrate Na 3 C 6 h 5 o 7

AR glacial acetic acid CH 3 COOH

AR Anhydrous sodium acetate CH 3 COONa

AR Xylose C 5 h 10 o 5

AR Xylan (C 5 h 8 o 4 ) n

AR sodium tartrate C 4 h 5 Na 2 o 6

AR tartaric acid C 4 h 6 o 6

AR Veratrol C 8 h 12 o 3

AR lactic acid C 3 h 6 o 3

AR sodium lactate C 3 h 5 o 3 Na

AR Manganese sulfate MnSO 4

AR sodium hydroxide NaOH AR hydrochloric acid H...

Embodiment 2

[0141] Example 2 : Identification of Geobacillus thermodenitrificans TB62 (Geobacillus thermodenitrificans)

[0142] 2.1 The main instruments of the experiment

[0143] Table 4 Instruments used in experiments

[0144]

[0145]

[0146] 2.2 Experimental steps

[0147] 2.2.1 DNA extraction

[0148] DNA was extracted using Sangon's "Ezup Column Bacterial Genomic DNA Extraction Kit Instructions". Please refer to the instruction manual for specific operation steps.

[0149] 2.2.2 PCR amplification

[0150] Use 2×Es Taq MasterMix for PCR amplification, and the reaction system is as follows:

[0151]

[0152] Reaction procedure:

[0153]

[0154] 2.2.3 PCR product electrophoresis results

[0155] Use 1% concentration of agarose gel electrophoresis, voltage 120V, electrophoresis 30min, load 1uL per hole, see the electrophoresis pattern Figure 13 .

[0156] like Figure 13 As shown, the results of PCR product electrophoresis indicated that the bacterial 16S rDN...

Embodiment 3

[0163] Example 3 : The application of the bacterial agent containing thermodenitrifying Geobacillus TB62 in promoting the maturity of compost

[0164] 3.1 Preparation of bacterial agent

[0165] The TB42 seed solution was inoculated into 1L of sterilized LB culture solution according to the inoculum amount of 1% (v / v), cultivated in a shaker (50°C, 120rmp) for 24h, and the culture solution was collected to be the bacterial agent. The bacteria-adding group was the experimental group (T), and the blank group (CK) was added with 1 L of sterilized LB culture solution.

[0166] 3.2 Composting experiment

[0167] Cow dung and sugarcane leaves are selected as compost raw materials, with a mass ratio of 17:3, totaling 20kg. After composting for 45 days, samples were taken at 0, 5, 10, 16, 23, 30, and 45 days, and parameters such as temperature, pH, moisture content, organic matter, Kjeldahl nitrogen, inorganic nitrogen, and DOM content were measured. The whole composting process ...

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Abstract

The invention belongs to the technical field of plant growth-promoting bacteria development, and concretely relates togeobacillus thermodenitrificans TB62 and application thereof in promoting compost maturity. The geobacillus thermodenitrificans TB62 is named as Geobacillus thermodenitrificans in taxonomy, is preserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms in January 05, 2017, and has the preservation No. CGMCC 13530. The geobacillus thermodenitrficans TB62 provided by the invention is firstly screened and separated from samples during a compost high temperature period, and is high in temperature resistant, fast in growth and propagation, and capable of producing lignocellulose-degrading enzyme; a microbial agent containing geobacillus thermodenitrificans TB62 can be beneficial to improving the compost temperature, accelerating organic matter degradation and water-dissolved organic matter (DOM) degradation, improving the content of Kjeldahl nitrogen, and promoting compost maturity; the geobacillus thermodenitrificans TB62 also has a function of biosurfactant production so as to be applicable for preparing a surface active agent.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a thermal denitrifying Geobacillus strain TB62 and its application in promoting compost maturity. Background technique [0002] Chemical fertilizers play a huge role in modern agricultural production. However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year. Residues of pesticides and chemical fertilizers have released a large amount of harmful substances to the environment, polluting soil, water sources and food, and posing a great threat to human health and living environment. The call for protecting the ecological environment and producing safe food is becoming stronger and stronger. Therefore, the development and utilization of new fertilizer sources that replace chemical fertilizers to meet the needs of the development of green agriculture and green food has become a top pri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12N9/02C12N9/08C12N9/42C12P19/14C05F15/00C05F17/00C05F11/08C12R1/01
CPCC05F3/00C05F17/00C12N1/20C12N9/0061C12N9/0065C12N9/2437C12P1/04C12P19/14C12Y110/03002C12Y111/01013C12Y302/01004C12N1/205C12R2001/01C05F11/00C05F11/08Y02W30/40
Inventor 李群良徐佳琦陆彦宇
Owner GUANGXI UNIV
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