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Phosphorus-dissolving penicillium and application thereof

A kind of Penicillium phospholyticum, the technology of describing Penicillium phospholyticum, applied in the direction of application, fungi, chemicals for biological control, etc., can solve problems such as unsatisfactory effects

Active Publication Date: 2017-07-07
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there have been studies using microbial methods to solve the above-mentioned situation of soil phosphorus deficiency, but the effect is not satisfactory

Method used

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  • Phosphorus-dissolving penicillium and application thereof
  • Phosphorus-dissolving penicillium and application thereof
  • Phosphorus-dissolving penicillium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Purification and cultivation of test strains

[0049] Pick the test strains stored at 4°C and cultivate them in the center of the PDA plate. The bacteriostatic agent is lactic acid (25%), and culture them upside down in a 28°C incubator. Purification culture.

Embodiment 2

[0050] Embodiment 2: Screening of highly efficient phosphorus-solubilizing bacterial strains

[0051] (1) Preliminary screening of high-efficiency phosphorus-solubilizing strains

[0052] Inoculate the strain on a solid medium containing insoluble inorganic phosphate for culture, and qualitatively detect whether the microorganism has phosphorus-solubilizing ability according to the growth of the colony and the size of the transparent circle around the colony. The specific steps are as follows:

[0053] Scrape the colonies into a 300mL Erlenmeyer flask filled with 100mL of sterile water, shake for 30min at 28°C and 160r / min to fully disperse the spore mass; use a pipette to draw the spore suspension and place it on a hemocytometer Count, and finally get a concentration of 10 8 cfu / mL spore suspension; pipette 50 μL of the spore suspension onto the PDA medium, then spread it with a sterile applicator, and culture it upside down in a 28°C incubator for 3 days; punch out with a h...

Embodiment 3

[0060] Embodiment 3: the bacterial strain identification of test fungus

[0061] Cultivate the phosphate-solubilizing fungus strains QM-6 and QM-10 on the PDA plate in a biochemical incubator for about a week, and then observe the colony morphology and the pigment production of the colony. Pick a small amount of hyphae and use milk phenol oil as the floating medium to make glass slides, and observe the morphology and growth mode of microspores and the morphology of conidiophores under an Olympus microscope. Refer to Chinese Fungi (Volume 35) - Penicillium and its related sexual genera, "Handbook of Fungal Identification" to observe the morphology.

[0062] The genomic DNA of the phosphorus-solubilizing fungal strains QM-6 and QM-10 was extracted by conventional methods, and the ITS sequence of the strain was amplified by PCR using the extracted DNA as a template. The primers for PCR are:

[0063] ITS1 sequence is: 5'-TCCGTAGGTGAACCTGCGG-3', its nucleotide sequence is shown i...

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Abstract

The invention belongs to the microorganism field. A solid culture medium taking Ca3(PO4)2 as a unique phosphorus source is utilized for screening a strain 14 with a phosphorus-dissolving effect, and the phosphorus-dissolving capacity of a to-be-tested phosphorus-dissolving strain is determined by virtue of an inorganic phosphorus liquid culture medium shake flask experiment. The inorganic phosphorus liquid culture medium shake flask experiment determines that the phosphorus-dissolving capacities of penicillium strains QM-6 and QM-10 are relatively high, and the maximum soluble phosphorus contents of the two corresponding strains are respectively 1239.21mg / L and 1386.93mg / L. The strains QM-6 and QM-10 are preliminarily determined as Penicillium oxalicum by virtue of an ITS sequence determination method. The determination of a greenhouse pot culture phosphorus-dissolving experiment and the analysis of a growth-promoting effect find that the phosphorus-dissolving strains QM-6 and QM-10 are capable of converting unavailable phosphorus which is difficult to be absorbed and utilized by plants in soil into soluble available phosphorus which can be directly absorbed and utilized and well promoting the growth of the plants and have important theoretical and practical significances to reduction of the application amount of phosphorus fertilizers.

Description

[0001] Technical field: [0002] The invention relates to a Penicillium phospholyticum and an application thereof, belonging to the field of biotechnology, in particular to a strain capable of dissolving phosphorus, in particular to two Penicillium strains QM-6 and QM-10 with high phosphorus-dissolving ability. [0003] Background technique: [0004] Phosphorus is one of the nutrient elements necessary for plant growth and development. It is an important component of nucleic acid, various enzymes, coenzymes, ATP, etc. in plants. Its role in plant growth and development is second only to nitrogen. At the same time, there are various ways for phosphorus element to participate in plant metabolism. It can not only increase the yield of crops, but also increase the stress resistance of plants and reduce plant diseases and insect pests. The phosphorus used by plants is mainly the available phosphorus in the soil, but more than 95% of the phosphorus in the soil is ineffective phosphor...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80
Inventor 于金凤王彩霞张莉刘春菊刘爱新毛志全
Owner SHANDONG AGRICULTURAL UNIVERSITY
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