Penicillium sclerotiorum for poisoning plant parasitic nematodes and preparation method and application of penicillium sclerotiorum

Abstract

The invention discloses penicillium sclerotiorum for poisoning plant parasitic nematodes and a preparation method and application of the penicillium sclerotiorum. The strain number of the penicillium sclerotiorum is D35, and the registering serial number of the penicillium sclerotiorum in the China General Microbiological Culture Collection Center is CGMCC No.12168.A plant parasitic nematode inhibitor which is extracted from cultures of the penicillium sclerotiorum D35 through ethyl acetate and dissolved in ethyl acetate serves as an active ingredient, the corrected death rate on meloidogyn incognita is 100%, and the average inhibition rate of the penicillium sclerotiorum D35 on meloidogyn incognita egg hatching is 99%. On the condition that no auxiliary is added into the solid fermentation cultures of the penicillium sclerotiorum D35, the prevention effect on a cucumber root-knot nematode disease at the 45 day reaches 83.9%, and the prevention effect on the cucumber root-knot nematode disease after 80 days still reaches 77.7%.Cucumber seedlings treated with the penicillium sclerotiorum D35 are developed in root system, few in root knot and normal in plant growth, and the penicillium sclerotiorum D35 is safe to cucumbers.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a Penicillium sclerotinum poisoning and killing plant parasitic nematodes and its preparation method and application. Background technique [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. Globally, the occurrence and damage of plant parasitic nematodes are becoming more and more serious with the development of agriculture and forestry and the change of farming system. It is estimated that the global annual losses caused by plant parasitic nematodes to agriculture and forestry production are as high as 157 billion U.S. dollars (AbadP, et al. 2008. Furthermore, the direct or indirect impact of nematodes on plants through interactions with other organisms is increasing dramatically. [0003] At present, there are more than 3,000 kinds of nematodes harmful to plants. In my cou...

AI Technical Summary

Problems solved by technology

At present, nematode control is still dominated by chemical control, but the surface of the nematode body is cuticle without cell structure, the nervous system is underdeveloped, the air permeability and water permeability of the nematode are poor, and the response to chemical substances is slow, so there are many chemical nematicides. It is a highly toxic pesticide, which is highly toxic to humans and animals and pollutes the environment. Many of them have been banned from being used in vegetables and fruits

Although there are some medium and low toxicity nematicides suitable for use in vegetable fields, there are few varieties, which adds difficulties to the control of nematodes. Therefore, it is imminent to develop efficient and safe bionematicides

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