Application of penicillium sclerotiorum in preparation of plant growth regulator or inducer

A plant growth regulator, the technology of Penicillium sclerotiorum, which is applied in the field of agriculture, can solve the problems that it is difficult to achieve the best, and achieve the effect of promoting the growth of underground roots, improving bioavailability, and promoting early germination

Active Publication Date: 2019-02-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use effect of plant growth regulators is affected by many factors, and it is difficult to achieve the best

Method used

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  • Application of penicillium sclerotiorum in preparation of plant growth regulator or inducer
  • Application of penicillium sclerotiorum in preparation of plant growth regulator or inducer
  • Application of penicillium sclerotiorum in preparation of plant growth regulator or inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Design experiment: Maize variety B73 (Yan, J. et al. Accumulation of 5-hydroxynorvaline in maize (Zea mays) leaves is induced by insect feeding and biotic stress, J. Exp Bot. 2015, 66, 593-602) was bred according to conventional methods to 3-4 leaves, and then use three pots of plants as blank control (control1, control2, control3), and three pots of plants as test group (test1, test2, test3); 150ml of PDB culture solution (a total of 3 plants, 50ml for each plant), and the blank group was directly poured into 150ml of PDB culture solution (a total of 3 plants, 50ml for each plant), and photographed on the same day. After that, water regularly and quantitatively every other day to ensure the normal growth of corn, and take photos for comparison after one month.

[0052] The result is as figure 1 As shown, it was found that the culture solution of Penicillium sclerotiorum was watered to grow better, the stems were stronger, and the leaves at the bottom were senescent sl...

Embodiment 2

[0054] Put 20 Nipponbare rice seeds (Yan J. et al. The Tyrosine Aminomutase TAM1 IsRequired for beta-Tyrosine Biosynthesis in Rice, Plant Cell, 2015, 27, 1265-1278) into blank culture medium (ie PDB culture medium) and Cultivate in the culture solution with Penicillium sclerotiorum, and observe the germination rate of the seeds after 4 days.

[0055] The photo result is as follows figure 2 As shown in A, from the results of data statistics (2B): the culture solution of Penicillium sclerotinum can significantly increase the germination rate of rice seeds.

Embodiment 3

[0057] When the rice variety Nipponbare (Yan J. et al. The Tyrosine Aminomutase TAM1 IsRequired for beta-Tyrosine Biosynthesis in Rice, Plant Cell, 2015, 27, 1265-1278) grows to 4 to 5 leaves, each plant is applied separately 50ml of culture fluid with Penicillium sclerotiorum and blank culture fluid (ie PDB culture fluid), repeated three times, respectively blank group (control) 1-3, test group (test) 1-3. And took pictures on the same day (July 29, 2017), and then took pictures on August 7, 2017 and August 20, 2017 for comparison.

[0058] The result is as image 3 As shown, it is easy to see from the results of the photos that the culture medium of Penicillium sclerotiorum can make the rice grow stronger, and the leaves are less yellow. Further analysis of the root growth of the underground parts, the results are as follows Figure 4 As shown, it is easy to see from the results of the photos that the root system of the test group applied with Penicillium sclerotiorum is m...

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Abstract

The invention discloses an application of penicillium sclerotiorum in preparation of a plant growth regulator or a plant growth inducer. Through different research objects, the application is found tohave the penicillium sclerotiorum (namely purslane penicillium sclerotiorum SCAUMCX01), penicillium sclerotiorum spores, a culture solution of the penicillium sclerotiorum and the extract of the culture solution thereof and has the advantages of promoting the early germination of seeds, promoting the growth of underground roots of crops, branching the over-ground parts of crops, improving the bioavailability of the crop, delaying the aging process and enhancing the growth of the crop, so that the plant growth regulator can be used as a plant growth regulator and an inducer. In addition, the penicillium sclerotiorum can also play a role in correspondingly regulating microbial communities and activating plant defense systems, thereby promoting crop growth and enhancing crop resistance.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and particularly relates to the application of Penicillium sclerotinum in the preparation of plant growth regulators or inducers. Background technique [0002] China is a big agricultural country, and the income from crops is the main source of income for most farmers. In order to increase economic income, farmers generally use methods to increase the use of chemical fertilizers, and at the same time spray a large amount of pesticides to reduce the harm of diseases and insect pests, so as to achieve the purpose of increasing income. However, long-term operation in this way will easily lead to land compaction and excessive chemical fertilizer and pesticide residues, which seriously threaten people's health. At the same time, resulting in further deterioration of the ecological environment. [0003] Plant growth regulators are a class of pesticides used to regulate plant growth and developmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/80
CPCA01N63/30C12R2001/80C12N1/145A01N63/36A01P21/00C12N1/14
Inventor 颜健黎平
Owner SOUTH CHINA AGRI UNIV
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