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Trichoderma viridea LW-1 capable of degrading ethyl acetate and application of trichoderma viridea LW-1

A technology of Trichoderma viride and ethyl acetate, which is applied in the field of biological treatment of environmental pollutants, can solve the problems of different structures and inability to degrade ethyl acetate, and achieve high-efficiency degradation ability and broad application prospects

Active Publication Date: 2015-04-29
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is reported in the patent that the Trichoderma viride can only degrade formaldehyde or degrade formaldehyde in the presence of other common carbon sources (glucose, sucrose, etc.), and does not mention whether other common industrial pollutants have the ability to degrade
Ethyl acetate and formaldehyde mentioned in the present invention belong to two types of substances with very different structures. Trichoderma viride that degrades formaldehyde cannot degrade ethyl acetate

Method used

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  • Trichoderma viridea LW-1 capable of degrading ethyl acetate and application of trichoderma viridea LW-1
  • Trichoderma viridea LW-1 capable of degrading ethyl acetate and application of trichoderma viridea LW-1
  • Trichoderma viridea LW-1 capable of degrading ethyl acetate and application of trichoderma viridea LW-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation, purification and identification of Trichoderma viride LW-1.

[0021] 1. Isolation and purification of Trichoderma viride LW-1.

[0022] Trichoderma viride (Trichoderma viride) LW-1 is a fungus domesticated and isolated from the activated sludge in the aeration tank of a sewage treatment plant in Hangzhou, Zhejiang. The specific steps are as follows: Take the activated sludge from the aeration tank of a sewage treatment plant in Hangzhou, Zhejiang. After washing with tap water for five times, air-expose for 48 hours to remove residual organic matter as much as possible. Prepare inorganic salt culture medium, use ethyl acetate as the only carbon source to carry out directional acclimation to activated sludge, replace fresh inorganic salt culture medium every 3 days, and it can be separated in about 40 days.

[0023] Prepare the initial concentration of ethyl acetate as 50mg / L inorganic salt medium, the preparation method is as follows: KH 2 PO 4 ...

Embodiment 2

[0030] Example 2: The ability of the strain LW-1 to utilize 95 carbon sources on the Biolog FF plate.

[0031] The Biolog automatic microbial identification system was used to investigate the metabolism of strains to 95 different carbon sources: the strains were inoculated on fungal plate medium, incubated at a constant temperature of 26°C for 5 days, and the bacterial cells on the plate were washed with a sterile cotton swab, and mixed with the inoculum (FF-IF) were mixed to make a bacterial suspension, and adjusted to 75% T / FF with a turbidimeter. Use an 8-hole electric pipette to add the bacterial suspension to each well of the Biolog FF microwell identification plate, 100 μl per well. Place the microwell identification plate in a 26°C incubator, and place it on a Biolog reader to read the results after culturing for 24h, 48h, 72h, 96h, 168h, and 240h, respectively. According to the analysis of metabolic fingerprint by Biolog reader, the strain LW-1 can utilize 43 kinds of...

Embodiment 3

[0037] Example 3: Optimizing environmental factors for the degradation of ethyl acetate by strain Trichoderma viride LW-1.

[0038] 1. Effects of different pH values ​​on the degradation of ethyl acetate by Trichoderma viride LW-1.

[0039] The pH value in the environment has a great influence on the growth of microorganisms. pH can directly affect the change of cell membrane charge and indirectly affect the ionization degree of nutrients. It has been shown that most compounds in the non-ionic state enter cells more easily through the cell wall than in the ionic state. In addition, enzymes in microorganisms can only be active under suitable pH conditions. Compared with bacteria and actinomycetes, fungi prefer to grow in an acidic environment.

[0040] From Figure 5 It can be concluded that when the initial concentration of ethyl acetate is 35.88mg / L, the degradation rate of pH 4.87 is the fastest, and the other degradation rates are pH5.57>pH3.25>pH3.88. When the shake fl...

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Abstract

The invention relates to trichoderma viridea LW-1 capable of degrading ethyl acetate and application of the trichoderma viridea LW-1 in degrading ethyl acetate, benzene, toluene, chlorobenzene, alpha-pinene or dichloromethane. The accession number of trichoderma viridea LW-1 is CCTCC NO: M2014176. The trichoderma viridea LW-1 provided by the invention can efficiently degrade target contaminants and is strong in endurance capability.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, in particular to a Trichoderma viride LW-1 strain capable of degrading ethyl acetate, and its ability to degrade ethyl acetate, benzene, toluene, chlorobenzene, α- application of pinene or dichloromethane. Background technique [0002] Ethyl acetate (molecular formula: CH 3 CO 2 CH 2 CH 3 ) is a common organic solvent, which is widely used in printing, adhesives and other production enterprises and industrial processes. Ethyl acetate is highly volatile and irritating to the eyes, skin, mucous membranes and upper respiratory tract. Prolonged exposure has an anesthetic effect on people and can cause dizziness and headache. Therefore, it is urgent to find effective countermeasures to prevent and control ethyl acetate pollution, which is of great significance to human health and environmental protection. [0003] Biological treatment of ethyl acetate pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34B01D53/84B01D53/72C12R1/885C02F101/34C02F101/32C02F101/36
CPCB01D53/72B01D53/84B01D2251/95B01D2257/702B01D2257/7027C02F3/34C02F3/348C02F2101/32C02F2101/34C02F2101/36C12N1/145C12R2001/885Y02A50/20
Inventor 成卓韦陈建孟於建明陆李超
Owner ZHEJIANG UNIV OF TECH
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