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Bacillus subtilis multifunctional bacterial strain and application thereof

A Bacillus subtilis, multifunctional technology, applied in the field of microorganisms, can solve the problems of lack of multifunctional microbial resources and low degradation rate, etc.

Active Publication Date: 2014-09-10
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The degradation rate of 15mg / kg chlorpyrifos is only 44.08%, the degradation rate is low, and the degradation rate of chlorpyrifos in field application is only 39.14%
[0009] It can be seen that the use of safe, economical and effective microbial treatment and remediation of environmental pollution caused by chlorpyrifos is a feasible and ecologically friendly method, but there are currently limited reports on the number of microorganisms in such resources, and higher concentrations (>2g / L) There are few reports on chlorpyrifos tolerance or degradation, and there are almost no research reports on related multifunctional microbial resources.

Method used

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  • Bacillus subtilis multifunctional bacterial strain and application thereof
  • Bacillus subtilis multifunctional bacterial strain and application thereof
  • Bacillus subtilis multifunctional bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Separation and Purification of Bacterial Strains

[0027] Obtain litter samples at the end of the soil freeze-thaw period from the original forest of Minjiang fir, take them back to the laboratory and store them at -4°C, and prepare the rest as litter powder (freeze-dried, chopped and passed through a 40-60 mesh sieve) and destroyed Store in polypropylene bags at -20°C.

[0028] In inorganic salt medium (inorganic salt medium: K 2 HPO 4 1g / L; NaH 2 PO 4 1g / L; (NH4) 2 SO 4 0.5g / L; MgSO 4 0.2g / L; CaCl 2 0.1g / L; FeSO 4 0.05g / L; MnSO 4 0.02g / L; dH 2O 1000mL; pH7.2; sterilized at 121°C for 20min), add 0.5g-4°C preserved litter samples per 100mL of culture medium, culture at 37°C, 180r / m shaker for 3 days, take the enriched solution by 5% The inoculum amount was inoculated into the same fresh medium, cultured under the same conditions for 3 days, after 3 times of transfer, take 0.1mL of culture solution and spread it on BM containing 0.1g / L aniline...

Embodiment 2

[0029] Identification of embodiment 2 strains

[0030] (1) Individual characteristics

[0031] The above-mentioned strain MZS1 is positive for Gram staining; the bacterium is rod-shaped, with blunt round ends, about 1.5-2.25 μm in length and 0.5-0.75 μm in width; it has a spore shape, and the spores are oval, mesozoic or nearly mesozoic , the sporangia are not obviously enlarged (see figure 1 ), flagellar periphysis (see figure 2 ); young cells grown on glucose nutrient agar were lightly stained with Lu's methylene blue staining solution, and there were no non-colored particles in the protoplast; the turning movement of the bacteria was observed on the phase contrast microscope.

[0032] (2) Group characteristics

[0033] The colony of the strain MZS1 grown on the beef extract peptone solid medium was irregular dry, off-white, opaque, slightly protruding, wrinkled, with irregular raised edges, and the colony was close to the medium, so it was difficult to pick. Stay filmy...

Embodiment 3

[0042] Example 3 Degradation of lignin and cellulose

[0043] Inoculate Bacillus subtilis MZS1 into LB liquid medium, and culture it with shaking at 37°C and 150r / min for 16h. According to the inoculum amount of 5% (V / V), inoculate the bacterial liquid to the fermentation medium (fermentation medium: BM medium containing 10g litter powder per L), after 15 days of fermentation at 37°C and 200r / m, the fermentation 4000rpm, centrifuged at room temperature for 30min, and dried at 65°C to measure the contents of lignin and cellulose. The degradation rates of lignin and cellulose were 67.82% and 41.51%, respectively, and the selectivity coefficient was 1.63. The results are shown in Table 2.

[0044] Degradation rate = total amount of lignin (or cellulose) degradation / total amount of lignin (or cellulose) in the original sample × 100%

[0045] Selectivity coefficient = lignin degradation rate / cellulose degradation rate

[0046] Table 2 Degradation rate of lignin and cellulose ...

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Abstract

The invention discloses bacillus subtilis capable of degrading lignin, cellulose and broad-spectrum dye and capable of degrading chlorpyrifos. The bacterial can still well grows in 5% chlorpyrifos, has an excellent degrading capability to chlorpyrifos in soil and water, can form a stable community relationship with indigenous strains to synergetically degrade chlorpyrifos.

Description

technical field [0001] The present invention relates to a multifunctional bacterial strain and its application, in particular to a strain of Bacillus subtilis (Bacillus subtilis) MZS1 CCTCC NO: M2014042 capable of degrading lignin, cellulose and broad-spectrum dyes, and capable of efficiently degrading chlorpyrifos, and the The application of the bacterial strain belongs to the technical field of microorganisms. Background technique [0002] 50%-60% of the biomass produced by photosynthesis is stored in plant tissues in the form of lignocellulose. When plants die or tissues wither, refractory components such as cellulose and lignin often become an important factor limiting the degradation of plant residues, which greatly affects the material cycle process of the ecosystem. Microbial decomposition is the main decomposition method of lignocellulose, so it is very important to screen efficient lignocellulose degrading microorganisms. [0003] Microorganisms capable of decompo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/125A62D101/04A62D101/28C02F101/38
Inventor 黄春萍吴福忠杨万勤张健
Owner SICHUAN AGRI UNIV
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