Exiguobacterium profundum having tolerance to vadaium and application of deep-sea exiguobacterium

A deep-sea microbacteria, a technology for use, applied in the field of microorganisms, to achieve the effects of high tolerance, fast growth speed, and high tolerance concentration

Active Publication Date: 2016-11-23
CHENGDU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a technical solution for the bioremediation of the environment polluted by heavy metals

Method used

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  • Exiguobacterium profundum having tolerance to vadaium and application of deep-sea exiguobacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The acquisition and separation of embodiment 1 bacteria

[0026] 1. The process of separating and purifying bacteria:

[0027] (1) Take the vanadium-containing polluted water in the drainage channel of the west slag yard of vanadium-titanium magnetite ore in Panzhihua City, Sichuan Province, and let it stand for 2 hours. Take 1 mL of the supernatant and add it to 50 mL of the liquid medium containing 10 mg / L of heavy metal vanadium, at 30 ° C. 120rpm culture 4d;

[0028] (2) Take 1 mL of the liquid culture medium cultured in (1) for 4 days and add it to 50 mL of liquid culture medium containing 50 mg / L of heavy metal vanadium, and culture it at 30° C. at 120 rpm for 4 days;

[0029] (3) Take 1 mL of the liquid culture medium cultivated in (2) for 4 days and add it to 50 mL of liquid culture medium containing 100 mg / L of heavy metal vanadium, and cultivate at 30° C. for 4 days at 120 rpm;

[0030] (4) Take 1 mL of the liquid culture medium cultivated in (3) for 4 days ...

Embodiment 2

[0037] Embodiment 2 bacterial morphological characteristics and biological identification

[0038] 1. Morphological characteristics

[0039] Pick the monoclonal colony obtained in Example 1 and inoculate it on the LB solid medium by streaking, place the plate upside down in a constant temperature incubator, incubate at 30° C. for 24 hours, and observe the colony morphology. Exiguobacterium profundum PFWT01 forms an irregular circular shape, with a smooth and moist surface, a slightly raised colony in the center, irregular edges, soft texture, orange-yellow, non-diffusion, see figure 1 .

[0040] 2. Physiological and biochemical characteristics

[0041] The monoclonal bacterium colony of embodiment 1 gained is inoculated in LB liquid basic medium, gets the Exiguobacterium profundum PFWT01 bacterial strain of logarithmic growth phase and carries out Gram staining, starch Hydrolysis test, oil hydrolysis test, methyl red test, H 2 S test, gelatin hydrolysis test, sugar ferment...

Embodiment 3

[0049] Embodiment 3: Bacteria is to the maximum tolerance concentration of heavy metal

[0050] Pick the monoclonal colonies obtained in Example 1, inoculate them in LB liquid medium, shake and culture at 30°C and 120rpm for 36h, and collect the bacterial concentration OD 600 For 1.2, the bacterial liquid was diluted according to the ratio of 1, 10, 100, 1 000, 10 000 to prepare gradient bacterial liquid; and LB solid medium with different concentrations of heavy metals was prepared. Take 50 μL of each concentration of bacterial solution to spread evenly on a plate with various heavy metal concentrations, incubate at 30°C, observe and record the growth of bacteria every 24 hours, and the lowest heavy metal concentration that inhibits its obvious growth within 120 hours is the maximum tolerance of the bacteria concentration.

[0051] The above-mentioned heavy metal solid media with different concentrations are respectively LB solid media with Cr, Pb, Cu, Ni, Co, Cd, Zn or V ad...

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Abstract

The invention belongs to the field of microorganisms, particularly relates to exiguobacterium profundum having tolerance to vanadium and application of exiguobacterium profundum and aims to provide a technical scheme for bioremediation of environment polluted by heavy metals. The collection number of exiguobacterium profundum PFWT01 is CCTCC NO:M2016175. Exiguobacterium profundum is highly tolerant to vanadium and has tolerance to heavy metals like Cr, Pb, Cu, Ni and Co, and tolerance to vanadium is up to 2000mg/L. Exiguobacterium profundum has capability of reducing pentavalent vanadium to tetravalent vanadium in a basic culture medium, conversion rate is up to 54.33%, toxicity of high-valence vanadium in a solution can be lowered, and exiguobacterium profundum can be applied to removing vanadium toxicity high-vanadium-toxicity liquid environment containing other heavy metal pollution especially, and has good application prospect in bioremediation of environment polluted by the heavy metals.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a deep-sea microbacterium resistant to vanadium and its application. Background technique [0002] Heavy metal pollution is becoming more and more serious. With the discharge of a large amount of industrial waste, agricultural wastewater and domestic sewage, heavy metal pollutants are also discharged together, causing heavy metal pollution in water. After heavy metals are discharged into the environment, they are not easy to degrade and can accumulate in the environment for a long time. Improper handling of heavy metals will lead to the release of a large number of potentially carcinogenic and mutagenic toxic compounds, resulting in heavy metal pollution that is very harmful to organisms and the environment. Therefore, it is very important to strengthen the control of heavy metal pollution and improve human health and ecosystem security. [0003] Vanadium is a silver-gray met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20C12N1/205C12R2001/01
Inventor 彭书明黄艺李迅倪师军阳小成谢鸿观王杰
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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