Heavy metal-tolerant bacterial strain and application thereof

A heavy metal and tolerance technology, applied in the direction of bacteria, water pollutants, water/sludge/sewage treatment, etc., can solve the problem of less bacteria and achieve the effect of strong vitality, wide adaptability and fast growth speed

Active Publication Date: 2012-11-07
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic and foreign scholars' research work on biosorption mainly focuses on fungi, algae, yeast, etc., while there are few domestic and foreign reports on bacteria as biosorbent materials.

Method used

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  • Heavy metal-tolerant bacterial strain and application thereof
  • Heavy metal-tolerant bacterial strain and application thereof
  • Heavy metal-tolerant bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Strain morphological characteristics and biological identification

[0034] 1. Morphological characteristics

[0035] Streak a single colony into the LB solid medium, place the plate upside down in a constant temperature incubator, culture at 30°C for 1 to 3 days, and observe the colony morphology. Enterobacter sp.T2 round spot, khaki, moist surface, no diffusion, see figure 1 .

[0036] 2. Physiological and biochemical characteristics

[0037] Gram stain, oxidase test, contact enzyme test, bacterial motility test, oxidative fermentation test of sugar alcohols (including glucose, lactose, galactose, fructose, xylose, sucrose, maltose, mannitol), utilization of sugar Alcohol gas production test, methyl red test, VP test, indole test, nitrate reduction test, H 2 S production test, arginine dihydrolase test, citrate utilization test, urease test, starch hydrolysis, litmus milk test, gelatin liquefaction test, the results are shown in Table 1.

[0038] Table 1 Physiologi...

Embodiment 2

[0042] Example 2: The maximum tolerance concentration of bacteria to heavy metals

[0043] The solid medium containing heavy metal ions was prepared separately, with the concentration starting from 0.1 mM / L and increasing by 0.5 mM. Streak and inoculate on fresh LB solid medium, culture for 48h at 30℃ for activation, pick a single colony to inoculate into LB liquid medium, culture with shaking at 30℃, 120r / min for 24h, collect bacterial concentration OD 600 For the bacterial solution of 1.5, press 10 0 , 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 Proportional dilution, respectively dipping in each concentration of bacterial liquid spot inoculation on the heavy metal solid medium. Cultivate at 30°C and observe and record the growth of bacteria every 24h. The minimum heavy metal concentration that inhibits its obvious growth within 120h is the maximum tolerable concentration of the bacteria. See Table 2.

[0044] Table 2 Statistical table of the strain tolerance concentration of the prese...

Embodiment 3

[0046] Example 3: Adsorption effect of adsorbent on lead

[0047] In the solutions with lead concentrations of 20, 50, 100, 150, and 200 mg / L and pH of 6, add the adsorbent prepared by the bacteria of the present invention that has been cultured for 12 hours and the dosage is 0.5 g / L. Adsorb for 90 minutes at 30°C, collect the supernatant by centrifugation, and determine the remaining lead ion concentration during adsorption equilibrium. With Pb 2+ Increase of initial mass concentration, Pb 2+ The removal rate decreases, and the equilibrium adsorption capacity increases, but the increase in adsorption capacity is getting smaller and smaller. The maximum adsorption capacity of the biosorbent can reach 81.22mg / g, see image 3 Shown.

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Abstract

The invention discloses a heavy metal-tolerant bacterial strain Enterobacter sp.T2 and application thereof. The collection number of the heavy metal-tolerant bacterial strain Enterobacter sp.T2 is CGMCCNo.4281. The heavy metal-tolerant bacterial strain Enterobacter sp.T2 can tolerate various heavy metals such as Pb<2+>, Cr<6+>, Mn<2+>, Zn<2+>, Cu<2+>, Ni<2+>, Cd<2+>, Co<2+>, Ag<+>, Hg<2+> and thelike, in particular, the Pb<2+> tolerance concentration and the Mn<2+> tolerance concentration are more than 2,000 mg / L and 1,000 mg / L respectively. A heave metal-removed adsorbent is prepared by activating, culturing, collecting, washing and drying the bacterial strains and the maximum adsorption quantity of the heavy metal Pb<2+> can reach 81.22 mg / g.

Description

Technical field [0001] The invention relates to a strain resistant to multiple heavy metals and an application method for preparing a biosorbent by using the strain. Background technique [0002] The development of industrialization has brought severe heavy metal pollution. Due to its non-degradable and bio-accumulative properties, it poses a huge threat to the ecological environment and human health. Traditional wastewater treatment methods mainly include precipitation, adsorption, electrolysis, membrane separation, etc. However, these methods generally have disadvantages such as high investment costs, secondary pollution, and poor treatment of trace heavy metals. Biosorption has gradually become a research hotspot due to its low cost and wide material sources. [0003] The microorganisms isolated and screened from the soil contaminated by heavy metals, due to the stress of heavy metals in the living environment, can not only have a certain tolerance to heavy metals, but also ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/20
Inventor 柴立元杨志辉苏艳蓉陈跃辉闵小波
Owner CENT SOUTH UNIV
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