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Acinetobacter and application thereof to biological treatment of heavy metal ions

A technology of heavy metal ions, Acinetobacter baumannii, applied in biological water/sewage treatment, microorganism-based methods, microorganisms, etc., can solve the problem that heavy metal adsorption research has not yet been reported.

Inactive Publication Date: 2011-02-16
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Acinetobacter is generally regarded as an opportunistic pathogen. In recent years, research has added new materials to the research on microbial radiation resistance and stress resistance, but the related heavy metal adsorption research has not been reported yet.

Method used

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  • Acinetobacter and application thereof to biological treatment of heavy metal ions
  • Acinetobacter and application thereof to biological treatment of heavy metal ions
  • Acinetobacter and application thereof to biological treatment of heavy metal ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027]Embodiment one: the cultivation and identification of Acinetobacter baumannii R9 (Acinetobacter baumannii R9) CGMCC No.3947

[0028] The present invention specifically provides a strain isolated and screened from the arid desert environment in Xinjiang, numbered R9, which has tolerance to various heavy metal ions, and the maximum tolerance to lead ions is 2200 mg / L. Currently tentatively named Acinetobacter baumannii R9. The growth and reproduction of the bacterial strains provided by the invention have no special requirements on nutrients, and can grow on common bacterial culture medium. Conventional microbial preservation techniques and methods such as slant passage method, freeze-drying method, and glycerol tubes can be used for preservation and viability testing. At present, the strain has been deposited in the International Depository of Microorganisms under the Budapest Treaty: the General Microorganism Center of China Committee for the Collection of Microorganism...

Embodiment 2

[0032] Embodiment 2: Tolerance of Acinetobacter baumannii R9 (Acinetobacter baumannii R9) CGMCC No.3947 to heavy metal ions

[0033] The isolated and purified strains were inoculated in Cu-containing 2+ Concentration 200mg / L-1000mg / L (every 100mg / L is a gradient), Zn 2+ Concentration 100mg / L-1200mg / L (every 200mg / L is a gradient), Pb 2+ Concentration 200mg / L-2200mg / L (every 200mg / L is a gradient), Hg 2+ Concentration 20mg / L-500mg / L (every 20mg / L is a gradient), Co 2+ On solid MM medium with a concentration of 50mg / L-400mg / L (every 50mg / L is a gradient), culture at a constant temperature of 30°C for 2 days, observe the growth of the strain, and detect the growth of the strain at different concentrations of various metal ions. For the test results of the highest tolerance of the strains to each metal ion, see the attached figure 1 shown.

[0034] From the detection results, the bacterial strains used in the present invention all have certain tolerance characteristics to fiv...

Embodiment 3

[0035] Embodiment three: thalline dosage is to thalline adsorption Pb 2+ Effect of effect

[0036] Pick a loop of fresh bacteria and inoculate it into a 500mL Erlenmeyer flask containing 50mL of liquid MM medium, culture at a constant temperature of 180r / min at 30°C for 1 day, collect the bacteria by centrifugation (4000r / min, 20min), and use After repeated washing with deionized water three times, the bacterial cells were collected for future use.

[0037] Accurately measure the concentration of 200mg / L Pb 2+ Aqueous solution 20mL, respectively weighed 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4g wet bacteria were added to the above Pb 2+ Disperse and mix in the aqueous solution, shake and absorb at room temperature for 30min, centrifuge at 13000r / min for 5min, take the supernatant to measure Pb 2+ ion concentration, and calculate its adsorption rate, the results are shown in the attached figure 2 shown.

[0038] The measurement results showed that with the increase of t...

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Abstract

The invention discloses acinetobacter baumannii R9 CGMCC No.3947 and application thereof to the adsorption of heavy metal ions. The acinetobacter baumannii is tolerant to five types of ions, namely, Hg<2+>, Cu<2+>, Co<2+>, Pb<2+> and Zn<2+>, wherein the tolerance concentrations to Pb<2+> and Zn<2+> are highest and are up to 2,200 mg / L and 1,500 mg / L respectively; the tolerances to Cu<2+> and Hg<2+> are up to 800 mg / L and 400 mg / L respectively; and the tolerance to Co<2+> is 300 mg / L. Through the method, lead ions are adsorbed by the growth of thalli and can be directly adsorbed by using wet thalli of which the maximum adsorption capacity is up to 39.56 mg / g. The acinetobacter has good adsorption effect in the application of the heavy metal ions and good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial strain resources and their application in biological treatment. Specifically, it relates to the technical field of an Acinetobacter and its application in the adsorption of heavy metal ions. Background technique [0002] Heavy metals refer to specific gravity greater than 4 or 5g / cm 3 There are about 45 kinds of metals, such as copper, zinc, lead, cobalt, manganese, cadmium, mercury and so on. Due to the increasing mining, smelting, processing and commercial manufacturing activities of heavy metals, various heavy metals enter the atmosphere, water and soil. The pollution of these toxic and harmful heavy metal elements will cause a series of heavy metals exceeding the standard and poisoning incidents. Even at low concentrations, they are easily absorbed by aquatic animals and plants and enter the food chain, thus causing high health risks to humans. [0003] At present, the commonly used metho...

Claims

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Application Information

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IPC IPC(8): C02F101/20C12N1/20C12R1/01C02F3/34
Inventor 朱静张志东谢玉清茆军宋素琴
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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