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A Radiation-resistant Aspergillus and Its Application in Adsorption of Cesium 137 Biological Treatment

A biological treatment and radiation-resistant technology, which is applied in the field of microbial strains for bioabsorption of radionuclides, radioresistant Aspergillus and its application in radionuclide adsorption, can solve the problems of the ability to repair radioactively polluted environments, and achieve The effect of good application prospects

Inactive Publication Date: 2016-08-31
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most bacteria are sensitive to radioactivity, their ability to remediate radioactively contaminated environments is limited
There are no reports about Aspergillus radiodurans and its application in radionuclide adsorption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Aspergillus ( Aspergillus sp. ) Isolation and culture of F77CGMCC No. 8382

[0042] 1. Separation: Aspergillus radiata used in the present invention ( Aspergillus sp. ) F77 was sampled and isolated from radioactively polluted soil in a certain place in Bayingoleng Mongolia Autonomous Prefecture, Xinjiang by the Institute of Microbiology Application, Xinjiang Academy of Agricultural Sciences. The microorganisms in the soil layer were isolated by traditional plate culture method, and the strain was purified by plate streak method. The culturing temperature, pH value, and medium were the enrichment conditions, and a batch of well-growing microbial strains were screened out, and a strain numbered F77 was selected from them.

[0043] Separation step: according to the gradient dilution method, take 10g soil samples in 90mL sterile saline, carry out gradient dilution after 30 ℃ of constant temperature shaking box activations for 30min, choose 10 -2 , 10 -3 , 10...

Embodiment 2

[0051] Example 2: Aspergillus ( Aspergillus sp. ) Molecular level identification of F77CGMCC No. 8382

[0052] 1. DNA extraction: The fungal extraction method is as follows:

[0053] (1) 200 mg of bacterial cells were ground in liquid nitrogen, and 3 ml of 3% CTAB extraction buffer was added; water bath at 65 °C for 45 min, and centrifugation at 4000 r / min at 4 °C for 20 min.

[0054] (2) Transfer the supernatant to a centrifuge tube, add 4 μl of 10 mg / ml protease, and incubate at 37°C for 1 h in a water bath.

[0055] (3) Add 800 μl of Tris saturated phenol, shake well, centrifuge at 13000 r / min for 10 min; take the supernatant.

[0056] (4) Add an equal volume of chloroform / isoamyl alcohol, shake well, and centrifuge at 13,000 r / min for 10 min; take the supernatant.

[0057] (5) Add 10 mg / ml RNase and treat in 37 ℃ water bath overnight.

[0058] (6) Add 800 μl chloroform / isoamyl alcohol, shake well, centrifuge at 13000 r / min for 10 min; take the supernatant.

[0059] (...

Embodiment 3

[0068] Example three: Aspergillus ( Aspergillus sp. ) Radiation resistance of F77CGMCC No. 8382

[0069] Microbial control of environmental radioactive pollution needs to meet two conditions, that is, it can survive in the radiation environment and have high adsorption selectivity for polluting nuclides. The fungus Aspergillus F77 used was extracted from radionuclide-contaminated soil, and after slant cultivation, 60 Co radioactive source was irradiated with γ-rays, and the cumulative irradiation dose was 10 kGy. By comparing the culture of irradiated samples and non-irradiated samples, it was found that its growth was not affected by irradiation, that is, Aspergillus F77 has strong radiation resistance. The radiation properties of F77 (gamma radiation) are described by Ferreira et al. [9] The established method is validated. The bacteria were cultured in PDA liquid medium to the stable stage, centrifuged at 4°C, washed with normal saline, and the suspension concentration...

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Abstract

The invention discloses a radioresistant aspergillus and its application in biological treatment of cesium 137 adsorption. Aspergillus ( Aspergillus sp. ) F77 CGMCC No. 8382, using the bacteria in the biological treatment of cesium adsorption, for Pb 2+ , Zn 2+ , Ni + The tolerance concentration is the highest, which can reach 1000 mg / L and 500 mg / L, respectively, and 500 mg / L; for Co 2+ , Cr 2+ , Hg 2+ The tolerance is next, both can reach 200 mg / L. Through this method, the growth of bacteria can be used to absorb cesium ions and radioactive cesium. It can also be directly absorbed by dry bacteria, and the maximum adsorption capacity can reach 44.5mg / g dry. Bacteria, which are used to adsorb Cs 137 The application in biological treatment has practical value and function.

Description

technical field [0001] The present invention relates to a microbial strain applied to the technical field of biological adsorption of radionuclides, in particular, the present invention relates to a radio-resistant Aspergillus and the technical field of its application in radionuclide adsorption. Background technique [0002] Urbanization, industrialization and population growth have caused a global energy crisis, and the need for sustainable development has made mankind increasingly dependent on new energy sources. Nuclear energy has been paid more and more attention by many countries due to its extremely low carbon dioxide emission and huge development potential. The rapid development of nuclear energy has also caused huge pressure on the global environment, making radioactive contamination one of the major environmental problems. Radioactive pollutants cause serious radioactive pollution in the local and surrounding environment, and pose a long-term and huge potential th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B01J20/00C12R1/66
Inventor 王玮王卫宪张志东谢玉清张丽娟徐辉顾美英朱静唐琦勇宋素琴王耀芹楚敏
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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