Method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1

Abstract

The invention relates to a method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1. The method comprises the steps of streaking and inoculating deinococcus radiodurans R1 in a TGY solid culture medium, picking out single colonies after culture and inoculating the single colonies in a TGY liquid culture medium, culturing the single colonies to a logarithmic growth period, inoculating the single colonies into a fresh TGY liquid culture medium according to the inoculation amount of 1%, culturing for 48h, collecting thallus cells, adding the thallus cells into abuffer solution containing 5-hydroxymethyl furfural, executing reaction at the temperature of 20-60 DEG C for 3-48h to obtain the 5-hydroxymethyl furoic acid. According to the method, the deinococcusradiodurans R1 is utilized as a biocatalyst, has high tolerance to the 5-hydroxymethyl furfural, can catalyze a high-concentration substrate to be selectively oxidized and synthesized into a target product, the yield reaches 86% or above, and the method has the advantages of higher substrate concentration, more excellent reaction efficiency and good selectivity.

Description

technical field [0001] The invention relates to a method for synthesizing 5-hydroxymethylfuroic acid by using Deinococcus radiodurans R1, which belongs to the technical field of microorganisms and chemical engineering. Background technique [0002] With the deepening of the sustainable development concept of advocating the replacement of petroleum resources with green energy and seeking environmentally friendly alternatives in the chemical industry in the 21st century, biomass energy and biotechnology occupy an increasingly critical role in the contemporary era of increasingly severe resource and environmental problems. status. 5-Hydroxymethylfurfural (HMF) is considered to be the most valuable and potential bio-based platform chemical to replace basic chemicals in the petrochemical industry. ) produced by dehydration. People's research on 5-hydroxymethylfurfural is mainly based on the following two important reasons: On the one hand, the HMF in the biological pretreatment...

AI Technical Summary

Problems solved by technology

A lot of progress has been made in the chemical conversion method, which is mainly prepared by the oxidation of HMF catalyzed by metal catalysts. However, the chemical reaction requires high temperature and pressure, and there are problems of poor product selectivity and substrate tolerance.

However, the use of biocatalysts can use HMF as a substrate under mild reaction conditions to convert HMFCA into HMFCA with high yield and high selectivity while maintaining high chemical purity and low by-products. Has toxic effects, most microorganisms have low substrate tolerance to HMF, and the catalytic conversion rate of HMF is slow. Strains that synthesize HMFCA are necessary

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