Method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1

A technology of Deinococcus radiodurans and hydroxymethylfuroic acid, which is applied in the field of microbiology and chemical engineering, can solve the problems of poor product selectivity substrate tolerance, slow HMF conversion speed, low tolerance concentration, etc., and achieve reaction efficiency Excellent, mild conditions, simple reaction process

Active Publication Date: 2019-07-09
NANJING UNIV OF TECH
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  • Application Information

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Problems solved by technology

A lot of progress has been made in the chemical conversion method, which is mainly prepared by the oxidation of HMF catalyzed by metal catalysts. However, the chemical reaction requires high temperature and pressure, and there are problems of poor product selectivity and substrate tolerance.
However, the use of biocatalysts can use HMF as

Method used

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  • Method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1
  • Method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1
  • Method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method utilizing Deinococcus radiodurans R1 to synthesize 5-hydroxymethylfuroic acid, comprising the steps of:

[0026] (1) Streak inoculation of Deinococcus radiodurans R1 on TGY solid medium (0.3% yeast, 0.5% peptone, 0.1% glucose, 1.8% agar), and culture at 30°C for 72 hours, the colony morphology of Deinococcus radiodurans R1 See figure 1 Pick a single colony and inoculate it in TGY liquid medium (yeast 0.3%, peptone 0.5%, glucose 0.1%), and cultivate it to the logarithmic growth phase at 30°C and 200r / min; Into fresh TGY liquid medium, cultured at 30°C and 200r / min for 48h, and collected bacterial cells;

[0027] (2) Add 0.25mmol HMF (100mM) to 2.5mL phosphate buffer (100mM, pH 7.4), mix well, and then mix the bacterial cells collected in step (1) at 120mg / mL (according to the wet weight of cells) The concentration was added, and reacted under the conditions of temperature 30°C and 850r / min, and the reaction was monitored by liquid chromatography. After 8 hours...

Embodiment 2

[0030] Change the temperature of reaction in step (2) to 40°C, and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 76.46%, and the yield of HMFCA was 98.13%.

Embodiment 3

[0032] Change the temperature of reaction in step (2) to 60° C., and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 57.05%, and the yield of HMFCA was 99.54%.

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Abstract

The invention relates to a method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1. The method comprises the steps of streaking and inoculating deinococcus radiodurans R1 in a TGY solid culture medium, picking out single colonies after culture and inoculating the single colonies in a TGY liquid culture medium, culturing the single colonies to a logarithmic growth period, inoculating the single colonies into a fresh TGY liquid culture medium according to the inoculation amount of 1%, culturing for 48h, collecting thallus cells, adding the thallus cells into abuffer solution containing 5-hydroxymethyl furfural, executing reaction at the temperature of 20-60 DEG C for 3-48h to obtain the 5-hydroxymethyl furoic acid. According to the method, the deinococcusradiodurans R1 is utilized as a biocatalyst, has high tolerance to the 5-hydroxymethyl furfural, can catalyze a high-concentration substrate to be selectively oxidized and synthesized into a target product, the yield reaches 86% or above, and the method has the advantages of higher substrate concentration, more excellent reaction efficiency and good selectivity.

Description

technical field [0001] The invention relates to a method for synthesizing 5-hydroxymethylfuroic acid by using Deinococcus radiodurans R1, which belongs to the technical field of microorganisms and chemical engineering. Background technique [0002] With the deepening of the sustainable development concept of advocating the replacement of petroleum resources with green energy and seeking environmentally friendly alternatives in the chemical industry in the 21st century, biomass energy and biotechnology occupy an increasingly critical role in the contemporary era of increasingly severe resource and environmental problems. status. 5-Hydroxymethylfurfural (HMF) is considered to be the most valuable and potential bio-based platform chemical to replace basic chemicals in the petrochemical industry. ) produced by dehydration. People's research on 5-hydroxymethylfurfural is mainly based on the following two important reasons: On the one hand, the HMF in the biological pretreatment...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12N1/20C12R1/01
CPCC12P17/04C12N1/20
Inventor 张志刚黄和苍然杨光慎立群张志东
Owner NANJING UNIV OF TECH
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