40 results about "Deinococcus radiodurans" patented technology

This invention relates to chromosomal engineering via DNA repair process. The process of the invention comprises the steps of: 1) submitting at least one source of biological activity, e.g. Deinococcus radiodurans, to radiation, desiccation and/or chemical treatment liable to damage the DNA, so as to substantially shatter its chromosomes into short fragments; 2) annealing complementary single strand tails extended by the synthesis templated on partially overlapping DNA fragments of said shattered chromosomes; 4) converting the resulting long linear DNA intermediates into intact circular chromosomes, by means of a RecA dependent homologous recombination; whereas at least one foreign source of genetic material, e.g. DNA, can be introduced during steps 2 and/or 3; and 4) optionally separating and collecting the recombined chromosomes thus obtained.

The invention discloses an enzyme activity starting and improving method of protease PprI. The enzyme activity starting of the protease PprI needs 2.0-5.0mM of Mn<2+> ions. An enzyme digestion reaction buffer solution contains 150-250mM OF NaCl, 10-50Mm of Tris-HcL 8.0, 0.1mM of DTT and 2.0-5.0mM of MnCl2. The combination of gene dr2574 and dr2340 promoter fragments with a substrate DdrO improves the enzyme digestion activity of the protease PprI, shortens the reaction time, and has positive contribution to future utilization of the enzyme.

The invention discloses a preparation method of gold nanoparticles, the gold nanoparticles and application. The preparation method comprises the following steps that (1) a deinococcus radiodurans rhzomorph solution is provided; (2) an Au3+-containing solution is provided; (3) the solutions in the step (1) and the step (2) are mixed to react; and (4) after reaction is completed, products are collected and purified, and the gold nanoparticles are obtained. The method for preparing the gold nanoparticles has the advantages of simpleness, convenience, controllability, high efficiency, energy conservation and environmental friendliness, and the synthesized gold nanoparticles are good in water solubility, high in purity degree, good in uniformity, moderate in size (29.83+/-4.18 nm), high in stability and high in antioxidant capacity, have the anticancer effect, and can be applied to the aspect of oxidation resistance or cancer inhibition.

The invention relates to a genetically engineered bacterium with high-yield electroactivity and environmental stress tolerance. The lactic dehydrogenase gene ldhA in a pseudomonas aeruginosa PAO1 genome is knocked out, and the PAOl knockout strain is obtained; then a global regulatory factor IrrE of the deinococcus radiodurans is guided into the ldhA-, and the genetic engineering strain ldhA--irrE is obtained. After the thallus treated through a chemical agent is inoculated to a microbial fuel cell, the generated voltage and power density are improved by 46.76%-53.33% than those of the wild type strain, the time for stabilizing a system is shortened by 28.57%, the internal resistance of the system is reduced by 12.66%, the voltage under polyethylene glycol treatment is 543, the power density is 207, the stabilizing time is 130 h, the internal resistance reaches 420.32, and the survival rate of the engineered strain under the hunger condition, the high-acid-base condition and high-salt condition are improved by one time than those of the wild type strain.

The present invention is directed to directly measure distribution in vivo and the frequency of generation in vivo of DNA strand breaks which induce cell death and mutations. The present inventors accomplished the present invention by providing a method for detecting a DNA strand break in a sample, which comprises a step of binding a PprA protein derived from Deinococcus radiodurans to a DNA strand break and a step of detecting the PprA protein which is bound to the DNA strand break; as well as by providing a kit for detecting a DNA strand break in a sample which comprises PprA proteins derived from Deinococcus radiodurans and a means for detecting a PprA protein which is bound to a DNA strand break.

The invention relates to a biological surfactant functional modification method for deinococcus radiodurans (DR). The biological surfactant functional modification method comprises the following steps: firstly embedding rice straws and rice husks in farmland soil for composting, carrying out enrichment cultivation, ferment cultivation and flat chemical cultivation primary screening to obtain a biological surfactant bacterial strain liquid, carrying out functional modification on the deinococcus radiodurans (DR) to obtain a biological surfactant functional modification deinococcus radiodurans (DR) adsorbent, and applying the adsorbent to uranium-polluted water body repair. The method disclosed by the invention uses natural resource biomasses as raw materials, and is free of toxicity or pollution to the environment, so that recycling and resource recovery of natural resources are realized. Meanwhile, the method is simple and mild in reaction conditions, the obtained adsorbent can enable the content of uranium in a uranium-polluted water body with the concentration of 0.45-0.50 mg/L to be decreased by 95.2% to 95.78%, and the method has excellent social and economic benefits.

The invention finds that a ytxH gene (DR0105) in Deinococcus radiodurans R1 has the function of improving the adversity resistance of prokaryotes and plants. The invention constructs recombinant vectors containing the gene and the recombinant vectors are respectively transferred into prokaryotic and eukaryotic host cells. Experiments prove that the ytxH gene (DR0105) can improve the salt tolerance of prokaryotes and the transgenic plants obtained after transplanting the gene into the plants also have salt tolerance.

A DNA molecule comprising the sequence set forth in SEQ ID NO: 1, a recombinant Pichia plasmid into which the DNA molecule is inserted, and a recombinant Pichia strain obtained by the transformation of the recombinant Pichia plasmid into a competent Pichia cell and efficiently expressing the PprI protein of Deinococcus radiodurans.

The invention discovers that dap gene (DRB0118) in Deinococcus radiodurans R1 improves the resistance of prokaryotes and plants. The invention constructs a recombinant vector comprising the gene; and the recombinant vector is transferred into prokaryotic and eukaryotic host cells. Experiments prove that: after the dap gene (DRB0118) is expressed in prokaryotic host cells and tobacco, the salt resistance of the prokaryotic host cells and the tobacco can be improved.

This invention provides novel truncation mutants of a phytochrome from the bacterium Deinococcus radiodurans. When expressed either in bacteria or mammalian cells, these mutant phytochromes spontaneously incorporate biliverdin, a ubiquitous intermediate in heme catabolism, and become fluorescent in the infrared (IR) region. These phytochromes are the first genetically encoded labels that can be excited by far-red light and fluoresce in the true IR (>700 nm). If these mutants instead incorporate protoporphyrin IX, an intermediate in heme biosynthesis, illumination now generates significant amounts of singlet oxygen. Singlet oxygen is useful because it can be used to kill individual proteins or cells, detect long-range protein-protein interactions, or generate electron-microscopic contrast. The invention also relates to methods of making and using such proteins and protein variants.

The invention discovers that trkB genes (DR1667) in Deinococcus radiodurans R1 are capable of enhancing resistance capability of prokaryotes and plants. Recombinant vectors comprising the trkB genes (DR1667) are constructed and are respectively transferred into prokaryotic and eukaryotic host cells. Experiments prove that salt tolerance of the prokaryotic host cells and rape can be enhanced after the trkB genes (DR1667) are expressed in the prokaryotic host cells and rape.

The invention relates to the technical field of biology, in particular to an application of PprI protein and its pharmaceuticals. The application of the PprI protein in the preparation of pharmaceuticals for controlling radiation injury in higher eukaryotes is provided. Researches discover that the PprI protein is significant to controlling acute radiation injury in human cells and mice and enhances the antioxidant ability and DNA damage repairing ability.

The invention discovers a gene with a hydroxylase function discovered from deinococcus radiodurans. The invention constructs a recombinant vector containing the gene, and the recombinant vector is expressed in prokaryotic host cell Escherichia coli. An experiment proves that after the gene is expressed in the prokaryotic host cell, carotenoid can be catalyzed to perform hydroxylation reaction. The gene can be used in the catalytic synthesis of the microorganisms.

The invention relates to an anti-blue-ray repairing composition containing camellia extract and application of the anti-blue-ray repairing composition. The composition contains the following components in percentage by weight: 25%-80% of the camellia extract, 2.5%-20%of Deinococcus radiodurans, 1%-10% of adenosine, 5%-15% of glycosylrutin, 2%-15% of a bilberry fruit extract, 1%-15% of beta-glucanand 0.2%-2% of dipotassium glycyrrhizinate. The composition is capable of weakening pigment induction of blue rays to skin in multiple ways, effectively defending subsequent chromatosis, relieving injury caused by the blue rays to the skin and keeping the skin elastic and glossy and is safe and efficient. The prepared anti-blue-ray repairing composition can be widely applied to products such as cosmetics including smoothing toners, emulsions and creams for repairing photoaging skin problems such as dryness, darkness and slackness.

The present invention relates to a composition with an antioxidant activity, comprising a first dry extract derived from plant cell cultures comprising superoxide dismutase of Sulfolobus solfataricus, a second dry extract derived from plant cell cultures comprising superoxide dismutase of Aeropyrum pernix and a third dry extract derived from plant cell cultures comprising superoxide dismutase from Deinococcus radiodurans, wherein said plant cell cultures are cultures of cells belonging to Solanum lycopersicum species.