A kind of method that utilizes Deinococcus radiodurans r1 to synthesize 5-hydroxymethyl furoic acid

A technology of Deinococcus radiodurans and hydroxymethyl furoic acid, which is applied in the fields of microorganisms and chemical engineering, can solve the problems of poor product-selective substrate tolerance, slow HMF conversion speed, and low tolerance concentration, etc., and achieves reaction efficiency. Excellent, mild conditions, simple reaction process effect

Active Publication Date: 2022-04-05
NANJING TECH UNIV
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Problems solved by technology

A lot of progress has been made in the chemical conversion method, which is mainly prepared by the oxidation of HMF catalyzed by metal catalysts. However, the chemical reaction requires high temperature and pressure, and there are problems of poor product selectivity and substrate tolerance.
However, the use of biocatalysts can use HMF as a substrate under mild reaction conditions to convert HMFCA into HMFCA with high yield and high selectivity while maintaining high chemical purity and low by-products. Has toxic effects, most microorganisms have low substrate tolerance to HMF, and the catalytic conversion rate of HMF is slow. Strains that synthesize HMFCA are necessary

Method used

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  • A kind of method that utilizes Deinococcus radiodurans r1 to synthesize 5-hydroxymethyl furoic acid
  • A kind of method that utilizes Deinococcus radiodurans r1 to synthesize 5-hydroxymethyl furoic acid

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Effect test

Embodiment 1

[0025] A method utilizing Deinococcus radiodurans R1 to synthesize 5-hydroxymethylfuroic acid, comprising the steps of:

[0026] (1) Streak inoculation of Deinococcus radiodurans R1 on TGY solid medium (0.3% yeast, 0.5% peptone, 0.1% glucose, 1.8% agar), and culture at 30°C for 72 hours, the colony morphology of Deinococcus radiodurans R1 See figure 1 Pick a single colony and inoculate it in TGY liquid medium (yeast 0.3%, peptone 0.5%, glucose 0.1%), and cultivate it to the logarithmic growth phase at 30°C and 200r / min; Into fresh TGY liquid medium, cultured at 30°C and 200r / min for 48h, and collected bacterial cells;

[0027] (2) Add 0.25mmol HMF (100mM) to 2.5mL phosphate buffer (100mM, pH 7.4), mix well, and then mix the bacterial cells collected in step (1) at 120mg / mL (according to the wet weight of cells) The concentration was added, and reacted under the conditions of temperature 30°C and 850r / min, and the reaction was monitored by liquid chromatography. After 8 hours...

Embodiment 2

[0030] Change the temperature of reaction in step (2) to 40°C, and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 76.46%, and the yield of HMFCA was 98.13%.

Embodiment 3

[0032] Change the temperature of reaction in step (2) to 60° C., and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 57.05%, and the yield of HMFCA was 99.54%.

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Abstract

The invention relates to a method for synthesizing 5-hydroxymethylfuroic acid by using Deinococcus radiodurans R1, inoculating Deinococcus radiodurans R1 on a TGY solid medium by streaking, picking a single colony and inoculating it on a TGY liquid medium after cultivation, After culturing to the logarithmic growth phase, insert it into fresh TGY liquid medium according to the inoculation amount of 1%, cultivate for 48 hours, collect the bacterial cells, add them to the buffer solution containing 5-hydroxymethylfurfural, and heat them at a temperature of 20-60 Reaction at ℃ for 3-48 hours to obtain 5-hydroxymethylfuroic acid. Deinococcus radiodurans R1 used in the present invention is used as a biocatalyst, which has high tolerance to 5-hydroxymethylfurfural, can catalyze the selective oxidation of high-concentration substrates to synthesize the target product, and the yield is more than 86%. The method of the present invention It has the characteristics of higher substrate concentration, better reaction efficiency and good selectivity.

Description

technical field [0001] The invention relates to a method for synthesizing 5-hydroxymethylfuroic acid by using Deinococcus radiodurans R1, which belongs to the technical field of microorganisms and chemical engineering. Background technique [0002] With the deepening of the sustainable development concept of advocating the replacement of petroleum resources with green energy and seeking environmentally friendly alternatives in the chemical industry in the 21st century, biomass energy and biotechnology occupy an increasingly critical role in the contemporary era of increasingly severe resource and environmental problems. status. 5-Hydroxymethylfurfural (HMF) is considered to be the most valuable and potential bio-based platform chemical to replace basic chemicals in the petrochemical industry. ) produced by dehydration. People's research on 5-hydroxymethylfurfural is mainly based on the following two important reasons: On the one hand, the HMF in the biological pretreatment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/04C12N1/20C12R1/01
CPCC12P17/04C12N1/20
Inventor 张志刚黄和苍然杨光慎立群张志东
Owner NANJING TECH UNIV
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