A kind of Deinococcus northwestii r15 and its application

A deinococcus and inoculum technology, applied to Deinococcus northwest and its application field, can solve the problems of poor product selectivity and substrate tolerance, slow catalytic HMF conversion rate, low tolerance concentration, etc., and achieve excellent reaction efficiency. , the effect of mild conditions and simple reaction process

Active Publication Date: 2021-01-29
NANJING TECH UNIV
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  • Claims
  • Application Information

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Problems solved by technology

A lot of progress has been made in the chemical conversion method, which is mainly prepared by the oxidation of HMF catalyzed by metal catalysts. However, the chemical reaction requires high temperature and pressure, and there are problems of poor product selectivity and substrate tolerance; the use of biocatalysts can be used in mild Under certain reaction conditions, HMF is used as a substrate to transform into HMFCA, which has high yield and high selectivity, while maintaining high chemical purity and low by-products. However, due to the toxic effect of 5-hydroxymethylfurfural on microbial cells, most microorganisms Usually, the concentration of substrate tolerance to HMF is low, and the catalytic HMF conversion rate is slow. Therefore, it is urgent to screen out a strain that has high tolerance to the substrate HMF and can efficiently catalyze the selective oxidation of HMF to synthesize HMFCA.

Method used

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  • A kind of Deinococcus northwestii r15 and its application
  • A kind of Deinococcus northwestii r15 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The application method of Deinococcus Northwest R15 in the synthesis of 5-hydroxymethylfuroic acid comprises the steps:

[0024] (1) Inoculate Deinococcus Northwest R15 in TGY medium (yeast 0.3%, peptone 0.5%, glucose 0.1%), and activate it at 30°C and 200r / min for 30h (see the colony diagram of Deinococcus Northwest R15 figure 1 ), inoculated into fresh TGY medium according to 1% inoculum size, cultured at 30°C and 200r / min for 48h, and collected bacterial cells;

[0025] (2) Add 0.25mmol HMF (100mM) to 2.5mL phosphate buffer (100mM, pH 7.4), mix well, and add the bacteria collected in step (1) at a concentration of 120mg / mL (based on wet cell weight). Somatic cells were reacted at 20°C and 850r / min, and the reaction was monitored by liquid chromatography. After 8 hours, the liquid chromatogram was shown in figure 2 (The retention times of HMFCA and HMF were 2.81 and 6.15 min, respectively), the conversion rate of HMF was 76.94%, and the yield of HMFCA was 99.44%.

...

Embodiment 2

[0028] Change the temperature of reaction in step (2) to 40°C, and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 99.85%, and the yield of HMFCA was 98.22%.

Embodiment 3

[0030] Change the temperature of reaction in step (2) to 60° C., and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 48.59%, and the yield of HMFCA was 97.38%.

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Abstract

The invention relates to northwest deinococcus R15. The northwest deinococcus R15 is preserved in CCTCC on march 14th, 2019, and a preservation number is CCTCC No: M 2019143. The northwest deinococcusR15 is used for catalyzed synthesis of 5-hydroxymethyl-furoic acid, firstly, a TGY culture medium is inoculated with the northwest deinococcus R15, and after activation and culture are conducted, bacterial cells are collected; then 5-hydroxymethylfurfural is added into a buffer solution with a pH of 6.0-10.0, after a mixture is mixed evenly, the bacterial cells are added, and at 20-60 DEG C, reaction is carried out for 3-48 h to obtain the 5-hydroxymethyl-furoic acid. The northwest deinococcus R15 has high tolerance towards the 5-hydroxymethylfurfural, and can catalyze high-concentration substrates for selective oxosynthesis of the target product, and the yield is over 90%. The synthesis method has the characters that the substrate concentration is higher, the reaction efficiency is moreexcellent, and the selectivity is good.

Description

technical field [0001] The invention relates to a strain of Deinococcus Northwest and its application, especially the application of Deinococcus Northwest R15 in catalyzing the selective oxidation of 5-hydroxymethylfurfural to synthesize 5-hydroxymethylfuroic acid, belonging to the fields of biocatalysis and chemical engineering. Background technique [0002] With the deepening of the sustainable development concept of advocating the replacement of petroleum resources with green energy and seeking environmentally friendly alternatives in the chemical industry in the 21st century, biomass energy and biotechnology occupy an increasingly critical role in the contemporary era of increasingly severe resource and environmental problems. status. 5-Hydroxymethylfurfural (HMF) is a chemical substance produced by the dehydration of glucose or fructose. The molecule contains a furan ring, an aldehyde group and a hydroxymethyl group. Condensation and other reactions to prepare a variet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/04C12R1/01
Inventor 黄和张志刚苍然杨光慎立群张志东
Owner NANJING TECH UNIV
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