A kind of Deinococcus northwestii r15 and its application
A deinococcus and inoculum technology, applied to Deinococcus northwest and its application field, can solve the problems of poor product selectivity and substrate tolerance, slow catalytic HMF conversion rate, low tolerance concentration, etc., and achieve excellent reaction efficiency. , the effect of mild conditions and simple reaction process
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Embodiment 1
[0023] The application method of Deinococcus Northwest R15 in the synthesis of 5-hydroxymethylfuroic acid comprises the steps:
[0024] (1) Inoculate Deinococcus Northwest R15 in TGY medium (yeast 0.3%, peptone 0.5%, glucose 0.1%), and activate it at 30°C and 200r / min for 30h (see the colony diagram of Deinococcus Northwest R15 figure 1 ), inoculated into fresh TGY medium according to 1% inoculum size, cultured at 30°C and 200r / min for 48h, and collected bacterial cells;
[0025] (2) Add 0.25mmol HMF (100mM) to 2.5mL phosphate buffer (100mM, pH 7.4), mix well, and add the bacteria collected in step (1) at a concentration of 120mg / mL (based on wet cell weight). Somatic cells were reacted at 20°C and 850r / min, and the reaction was monitored by liquid chromatography. After 8 hours, the liquid chromatogram was shown in figure 2 (The retention times of HMFCA and HMF were 2.81 and 6.15 min, respectively), the conversion rate of HMF was 76.94%, and the yield of HMFCA was 99.44%.
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Embodiment 2
[0028] Change the temperature of reaction in step (2) to 40°C, and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 99.85%, and the yield of HMFCA was 98.22%.
Embodiment 3
[0030] Change the temperature of reaction in step (2) to 60° C., and the rest are the same as in Example 1. After 8 hours of reaction, the conversion of HMF was 48.59%, and the yield of HMFCA was 97.38%.
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