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Preparation method of fumaric acid and preparation method of amino acid

A technology of fumaric acid and furoic acid, applied in chemical instruments and methods, fermentation, physical/chemical process catalysts, etc., to achieve mild conditions, realize repeated utilization, and overcome unfriendly effects on the environment

Active Publication Date: 2021-09-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the synthesis of L-Asp and β-Ala from bio-based furfural has not been reported

Method used

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  • Preparation method of fumaric acid and preparation method of amino acid
  • Preparation method of fumaric acid and preparation method of amino acid
  • Preparation method of fumaric acid and preparation method of amino acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Resin pretreatment method: soak about 10g macroporous weakly basic styrene-based anion resin (DVB), D201 macroporous strongly basic styrene-based anion-exchange resin, 717 strongly basic styrene-based anion-exchange resin in acetonitrile (50mL) for 30 minutes, filtered and then immersed in methanol (20mL) for 30 minutes and filtered. The separated resin was dried with a rotary evaporator at 50°C until it was free flowing, and then dried in a hot air oven at 50°C for 1 hour.

[0040] The preparation method of immobilized EY: 1 g of macroporous weakly basic styrene-based anion exchange resin (DVB), D201 macroporous strongly basic styrene-based anion-exchange resin, 717 strong-based styrene-based anion-exchange resin, 300 mg of EY Red Y (eosinY, EY) was added into 40mL water and mixed evenly, and adsorbed at 30°C and 100rpm for 10h. The catalyst was filtered, and the filtrate was collected in a 250mL standard flask. The obtained catalyst was ultrasonically cleaned in 10 ...

Embodiment 2

[0042] In 4mL phosphate buffer (200mM, pH 7.0), add 50mg / mL recombinant bacteria E.coli-CtVDH2-NOX (wet weight) and furfural (final concentration 50mM), place in a constant temperature shaker (30°C, 150rpm) React for 2 hours, remove the cells (8000rpm, 4°C, centrifuge for 5min), adjust the pH to 9.0, add the dye EY, place it under 30W 530nm-540nm green light (LED light) to react, the reaction temperature is 25°C, 200rpm, after 24h of reaction , the yield of fumaric semialdehyde (Fumaric semialdehyde, FSA) was 39%.

Embodiment 3

[0044] In 4mL phosphate buffer (200mM, pH 7.0), add 50mg / mL recombinant bacteria E.coli-CtVDH2-NOX (wet weight), and furfural (final concentration 50mM), place in a constant temperature shaker (30°C, 150rpm) React in medium for 2 hours, remove the cells, add the macroporous weakly basic styrene-based anion resin (DVB) prepared in Example 1 to immobilize EY (EY@DVB), the amount of EY@DVB added is 2mol% (final concentration 1mM), Place it under 30W 530nm-540nm green light (LED lamp) for reaction, the reaction temperature is 25°C, 200rpm, after 24 hours of reaction, the yield of fumaric acid semialdehyde is 83%.

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Abstract

The invention belongs to the field of biological catalysis and biological manufacturing, and discloses a preparation method of fumaric acid and a preparation method of amino acid. The preparation method of the fumaric acid comprises the following steps: (1) adding recombinant bacteria E.coli-CtVDH2-NOX and furfural into a buffer solution for reaction, and removing cells to obtain furoic acid; (2) adding eosin Y immobilized by macroporous basic resin into the furoic acid or adjusting the pH value of the furoic acid to be alkaline, then adding the eosin Y or rose bengal, and performing illumination reaction; and (3) adjusting the pH value of the reaction liquid obtained in the step (2) to be acidic, adding laccase and TEMPO, and reacting to obtain the fumaric acid. The preparation method of the amino acid includes combining photocatalysis and biological catalysis for catalyzing oxidation of furfural to prepare the amino acid. The preparation methods have the advantages of high selectivity, mild reaction conditions, environment friendliness, high reaction speed, easiness in recycling of the catalyst, and the like.

Description

technical field [0001] The invention belongs to the field of biocatalysis and biomanufacturing, and in particular relates to a method for preparing amino acids by catalytically converting furfural in one pot using photo / biocatalysis. Background technique [0002] Furfural is an important bio-based platform compound (Green Chem. 2010, 12, 539), which is mainly prepared from agricultural wastes such as food crop residues or wood wastes. Currently, the global annual output of furfural is about 280,000 tons (Energy Environ. Sci. 2016, 9, 1144). Among them, my country is the main producer of furfural, and 70% of the world's furfural is produced by my country. Furfural molecules contain aldehyde groups and aromatic heterocycles, which can be further transformed into various high value-added chemicals. L-aspartic acid (L-aspartic acid, L-Asp) has important application value in medicine, polymer, food and other fields. L-Asp can be used not only as a sour taste regulator, but als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/06C12P13/20C12P7/46B01J31/08B01J35/00
CPCC12P13/06C12P13/20C12P7/46B01J31/08B01J35/39Y02P20/584
Inventor 李宁黄依敏宗敏华
Owner SOUTH CHINA UNIV OF TECH
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