Method for Catalytic Synthesis of 5-Hydroxymethyl Furoic Acid Using Deinococcus Urumqi

A technology of hydroxymethylfuroic acid and Deinococcus, which is applied in the field of biocatalysis and chemical engineering, can solve the problems of poor product selectivity and substrate tolerance, slow catalytic conversion of HMF, low tolerance concentration, etc. Excellent efficiency, mild conditions and simple reaction process

Active Publication Date: 2022-03-29
NANJING TECH UNIV
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  • Claims
  • Application Information

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Problems solved by technology

A lot of progress has been made in the chemical conversion method, which is mainly prepared by the oxidation of HMF catalyzed by metal catalysts. However, the chemical reaction requires high temperature and pressure, and there are problems of poor product selectivity and substrate tolerance; the use of biocatalysts can be used in mild Under certain reaction conditions, HMF is used as a substrate to transform into HMFCA, which has high yield and high selectivity, while maintaining high chemical purity and low by-products. However, due to the toxic effect of 5-hydroxymethylfurfural on microbial cells, large Some microorganisms usually have a low substrate tolerance concentration for HMF, and the conversion rate of catalytic HMF is slow

Method used

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  • Method for Catalytic Synthesis of 5-Hydroxymethyl Furoic Acid Using Deinococcus Urumqi
  • Method for Catalytic Synthesis of 5-Hydroxymethyl Furoic Acid Using Deinococcus Urumqi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method utilizing Deinococcus Urumqi to catalyze the synthesis of 5-hydroxymethylfuroic acid, comprising the steps of:

[0028] (1) Inoculate Deinococcus Urumqi R12 in TGY medium (yeast 0.3%, peptone 0.5%, glucose 0.1%), and activate it at 30°C and 200r / min for 30h (the colony morphology of Deinococcus Urumqi R12 is shown in figure 1 ), inoculated into fresh TGY medium according to 1% inoculum size, cultured at 30°C and 200r / min for 48h, and collected bacterial cells;

[0029] (2) Add 0.25mmol HMF (100mM) to 2.5mL phosphate buffer (100mM, pH 7.4), mix well, and add the bacteria collected in step (1) at a concentration of 120mg / mL (based on wet cell weight). Somatic cells were reacted at 25°C and 850r / min, and the reaction was monitored by liquid chromatography. After 4 hours, the liquid chromatogram was shown in figure 2 (The retention times of HMFCA and HMF are 2.81 and 6.15 min, respectively), the conversion rate of HMF is 58.57%, and the yield of HMFCA is 99.50%. ...

Embodiment 2

[0032] Change the temperature of reaction in step (2) to 40°C, and the rest are the same as in Example 1. After 4 hours of reaction, the conversion of HMF was 77.04%, and the yield of HMFCA was 99.49%.

Embodiment 3

[0034] Change the temperature of reaction in step (2) to 60° C., and the rest are the same as in Example 1. After 4 hours of reaction, the conversion of HMF was 47.88%, and the yield of HMFCA was 99.09%.

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Abstract

The invention relates to a method for catalyzing and synthesizing 5-hydroxymethyl furfural by using Deinococcus Urumqi, inoculating Deinococcus Urumqi R12 on TGY medium, and collecting bacterial cells after activation and cultivation; adding 5-hydroxymethylfurfural Put it into a buffer solution with a pH of 6.0-10.0, mix evenly, add the collected bacterial cells, and react at a temperature of 25-60°C for 3-48 hours to obtain 5-hydroxymethylfuroic acid. The biocatalyst Deinococcus Urumqi used in the invention has high tolerance to 5-hydroxymethylfurfural, can catalyze the selective oxidation of a high-concentration substrate to synthesize a target product, and the yield is over 89%. The scheme of the present invention overcomes the disadvantage that the chemical catalyst in the prior art is not friendly to the environment, and has the characteristics of higher substrate concentration, better reaction efficiency and good selectivity.

Description

technical field [0001] The invention relates to a method for catalyzing and synthesizing 5-hydroxymethylfuroic acid by using Deinococcus Urumqi, belonging to the fields of biocatalysis and chemical engineering. Background technique [0002] Hydroxymethylfurfural (HMF) is a chemical substance produced by the dehydration of glucose or fructose. The molecule contains a furan ring, an aldehyde group and a hydroxymethyl group. Its chemical properties are relatively active and can be prepared by reactions such as oxidation, hydrogenation and condensation. A variety of derivatives are important fine chemical raw materials, and are also considered to be the most valuable and potential bio-based platform chemicals to replace basic chemicals in the petrochemical industry. [0003] People's research on 5-hydroxymethylfurfural is mainly based on the following two important reasons: On the one hand, the HMF in the biological pretreatment hydrolyzate can cause the growth inhibition of man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/04C12N1/20C12R1/01
Inventor 黄和张志刚苍然慎立群杨光张志东
Owner NANJING TECH UNIV
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