Method for Catalytic Synthesis of 5-Hydroxymethyl Furoic Acid Using Deinococcus Urumqi
A technology of hydroxymethylfuroic acid and Deinococcus, which is applied in the field of biocatalysis and chemical engineering, can solve the problems of poor product selectivity and substrate tolerance, slow catalytic conversion of HMF, low tolerance concentration, etc. Excellent efficiency, mild conditions and simple reaction process
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Embodiment 1
[0027] A method utilizing Deinococcus Urumqi to catalyze the synthesis of 5-hydroxymethylfuroic acid, comprising the steps of:
[0028] (1) Inoculate Deinococcus Urumqi R12 in TGY medium (yeast 0.3%, peptone 0.5%, glucose 0.1%), and activate it at 30°C and 200r / min for 30h (the colony morphology of Deinococcus Urumqi R12 is shown in figure 1 ), inoculated into fresh TGY medium according to 1% inoculum size, cultured at 30°C and 200r / min for 48h, and collected bacterial cells;
[0029] (2) Add 0.25mmol HMF (100mM) to 2.5mL phosphate buffer (100mM, pH 7.4), mix well, and add the bacteria collected in step (1) at a concentration of 120mg / mL (based on wet cell weight). Somatic cells were reacted at 25°C and 850r / min, and the reaction was monitored by liquid chromatography. After 4 hours, the liquid chromatogram was shown in figure 2 (The retention times of HMFCA and HMF are 2.81 and 6.15 min, respectively), the conversion rate of HMF is 58.57%, and the yield of HMFCA is 99.50%. ...
Embodiment 2
[0032] Change the temperature of reaction in step (2) to 40°C, and the rest are the same as in Example 1. After 4 hours of reaction, the conversion of HMF was 77.04%, and the yield of HMFCA was 99.49%.
Embodiment 3
[0034] Change the temperature of reaction in step (2) to 60° C., and the rest are the same as in Example 1. After 4 hours of reaction, the conversion of HMF was 47.88%, and the yield of HMFCA was 99.09%.
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