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Biological surfactant functional modification method for deinococcus radiodurans (DR) and application thereof

A technology of Deinococcus radioresistant and biological surface, which is applied in the field of bioremediation of uranium-contaminated water bodies, can solve problems such as difficult separation, influence on the actual effect of repairing uranium-contaminated water bodies, small difference in density between bacteria and water, and achieve mechanical strength High, overcoming the problem of radiation resistance resistance, the effect of easy heating conditions

Active Publication Date: 2016-04-20
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the current domestic and foreign development of Deinococcus radiodurans DR There are few reports on remediation of uranium-contaminated water bodies
In addition, Deinococcus radiodurans DR It is easy to grow in a suspended state in the uranium-containing wastewater solution, and the density difference between the bacteria and water is small, and it is difficult to separate from the water, which affects the actual effect of repairing uranium-contaminated water.

Method used

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  • Biological surfactant functional modification method for deinococcus radiodurans (DR) and application thereof
  • Biological surfactant functional modification method for deinococcus radiodurans (DR) and application thereof
  • Biological surfactant functional modification method for deinococcus radiodurans (DR) and application thereof

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Experimental program
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Effect test

Embodiment 1

[0037] (1) Deinococcus radiodurans DR Preparation of bacteria

[0038] Deinococcus radiodurans to be kept in the laboratory DR Streak inoculation on the pre-prepared solid TGY medium, use the inoculation loop to pick a single strain of Deinococcus radiodurans DR Inoculate in Erlenmeyer flasks and shake the flasks until early logarithmic growth, then inoculate at 0.5% inoculum and transfer to medium in large flasks, and continue to cultivate at 25°C for 20 hours. Deinococcus radiodurans DR After the cell culture is stabilized, collect Deinococcus radiodurans by centrifugation DR Bacteria were centrifuged at 7000r / min for 10min to collect fresh Deinococcus radiodurans DR bacteria. Then use a phosphate buffer solution with a pH value of 7 to resuspend the bacteria, shake it well, and centrifuge again to collect Deinococcus radiodurans DR Bacteria were centrifuged at 8000r / min for 15min, and then the collected Deinococcus radiodurans DR The bacteria were placed in a vacuum ...

Embodiment 2

[0047] (1) Deinococcus radiodurans DR Preparation of bacteria

[0048] Deinococcus radiodurans to be kept in the laboratory DR Streak inoculation on the pre-prepared solid TGY medium, use the inoculation loop to pick a single strain of Deinococcus radiodurans DR Inoculate in the Erlenmeyer flask and shake the flask to culture to the early logarithmic growth stage, then inoculate and transfer to the medium in the large bottle according to the inoculum amount of 0.5%, and continue to cultivate at 30°C for 25h. Deinococcus radiodurans DR After the cell culture is stabilized, collect Deinococcus radiodurans by centrifugation DR Bacteria were centrifuged at 7000r / min for 10min to collect fresh Deinococcus radiodurans DR bacteria. Then use a phosphate buffer solution with a pH value of 7 to resuspend the bacteria, shake it well, and centrifuge again to collect Deinococcus radiodurans DR Bacteria were centrifuged at 8000r / min for 15min, and then the collected Deinococcus radiod...

Embodiment 3

[0057] (1) Deinococcus radiodurans DR Preparation of bacteria

[0058] Deinococcus radiodurans to be kept in the laboratory DR Streak inoculation on the pre-prepared solid TGY medium, use the inoculation loop to pick a single strain of Deinococcus radiodurans DR Inoculate in Erlenmeyer flasks and shake the flasks until early logarithmic growth, then inoculate at 1% inoculum and transfer to medium in large flasks, and continue to cultivate at 35°C for 30h. Deinococcus radiodurans DR After the cell culture is stabilized, collect Deinococcus radiodurans by centrifugation DR Bacteria were centrifuged at 7000r / min for 10min to collect fresh Deinococcus radiodurans DR bacteria. Then use a phosphate buffer solution with a pH value of 7 to resuspend the bacteria, shake it well, and centrifuge again to collect Deinococcus radiodurans DR Bacteria were centrifuged at 8000r / min for 15min, and then the collected Deinococcus radiodurans DR The bacteria were placed in a vacuum freezer...

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Abstract

The invention relates to a biological surfactant functional modification method for deinococcus radiodurans (DR). The biological surfactant functional modification method comprises the following steps: firstly embedding rice straws and rice husks in farmland soil for composting, carrying out enrichment cultivation, ferment cultivation and flat chemical cultivation primary screening to obtain a biological surfactant bacterial strain liquid, carrying out functional modification on the deinococcus radiodurans (DR) to obtain a biological surfactant functional modification deinococcus radiodurans (DR) adsorbent, and applying the adsorbent to uranium-polluted water body repair. The method disclosed by the invention uses natural resource biomasses as raw materials, and is free of toxicity or pollution to the environment, so that recycling and resource recovery of natural resources are realized. Meanwhile, the method is simple and mild in reaction conditions, the obtained adsorbent can enable the content of uranium in a uranium-polluted water body with the concentration of 0.45-0.50 mg / L to be decreased by 95.2% to 95.78%, and the method has excellent social and economic benefits.

Description

technical field [0001] The invention relates to the field of bioremediation treatment of uranium-contaminated water bodies, specifically using a biosurfactant to treat Deinococcus radiodurans DR A new method for functional modification and its application in the remediation of uranium-contaminated water bodies. Background technique [0002] The environment is the space on which human beings live, and the material basis and necessary conditions for human production activities. The harmonious relationship between human beings and the natural environment is the basic guarantee for the sustainable and stable development of society. With the continuous development of social economy and the continuous improvement of human development and creativity, the intensity and influence of the transformation of the natural environment are also increasing. [0003] The development of the nuclear industry, the wide application of nuclear technology, and other activities in the fields of ind...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30C02F3/34C02F101/20
CPCB01J20/24C02F3/34C02F2101/006C02F2101/20
Inventor 肖方竹彭国文符建文何淑雅黄波唐艳余丽梅蒲移秋
Owner NANHUA UNIV
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