Preparation method of gold nanoparticles, gold nanoparticles and application
A nano-gold and reaction technology, applied in the biological field, can solve the problems of limiting the wide application of carotenoids, and achieve the effects of strong antioxidant capacity, moderate size and wide source.
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Embodiment 1
[0041] Bacterial culture and preparation of nano gold synthesis agent.
[0042] (1) Bacterial culture: Deinococcus radiodurans was plated and activated for culture, a single colony was picked and inoculated in 5mL TGY medium, and cultivated overnight at 32°C on a shaker with a shaker speed of 220rpm; then press 1:100 The proportion of the transfer to 1L culture flask (containing 500mL TGY medium), cultivated at 32 ℃, 220rpm for 24h to the bacterial stationary phase (OD 600nm = 1.0).
[0043] (2) Collecting and washing the bacteria: After the bacteria are cultivated to a stable period, they are collected by centrifugation at 8000 g for 10 minutes to obtain fresh bacteria. It was resuspended in 0.01M phosphate buffer solution with a pH of 7.2, fully shaken, centrifuged at 8000 g for 10 min, and the cells were collected.
[0044] (3) Preparation of nano-gold synthesis agent: shake the collected bacteria with pre-cooled acetone / methanol solvent (2 / 1, v / v) for 30 minutes at room ...
Embodiment 2
[0046] Bacterial culture and preparation of nano gold synthesis agent.
[0047] (1) Bacterial culture: Deinococcus radiodurans was plated and activated for culture, a single colony was picked and inoculated in 5mL TGY medium, and cultivated overnight at 30°C on a shaker with a shaker speed of 200rpm; then press 1:100 The proportion of the transfer to 1L culture flask (containing 500mL TGY medium), cultivated at 30 ℃, 200rpm for 24h to the bacterial stationary phase (OD 600nm = 1.2).
[0048] (2) Collecting and washing the bacterial cells: After the bacteria are cultivated to a stable period, they are collected by centrifugation at 10000 g for 8 minutes to obtain fresh bacterial cells. It was resuspended in 0.01M phosphate buffer solution with a pH of 7.2, shaken thoroughly, centrifuged at 10000 g for 8 min, and the cells were collected.
[0049] (3) Preparation of nano-gold synthetic agent: shake the collected bacteria with pre-cooled acetone / ethanol solvent (4 / 1, v / v) for 3...
Embodiment 3
[0051] Preparation of gold nanoparticles based on hydroxylated carotenoids.
[0052] (1) Preparation of synthetic reaction solution: dissolve 1.5 mg of radioresistant deinococcus (prepared in Example 1) in 10 mL of ethanol / water solvent (1 / 9, v / v), add 1 mM Au 3+ Solution (auric chloride, HAuCl 4 ·3H 2 O), at room temperature (pH 7, 25°C) to make it fully mixed and reacted.
[0053] (2) Monitoring the reaction process: As the reaction proceeds, the color of the solution changes and gradually tends to be stable. After 2 hours of reaction, a wavelength scan is performed (there is a peak at 539 nm).
[0054] (3) Purification of nano-gold solution: filter the above-mentioned red-purple solution with a 0.22 μm syringe filter, centrifuge at 15000 g for 60 min, collect the precipitate, wash with distilled water several times (the water solubility of nano-gold is better), and use a cut-off of 8KD The dialysis bag was dialyzed for 24 hours to remove the remaining reactants, and the ...
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