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Preparation method of gold nanoparticles, gold nanoparticles and application

A nano-gold and reaction technology, applied in the biological field, can solve the problems of limiting the wide application of carotenoids, and achieve the effects of strong antioxidant capacity, moderate size and wide source.

Active Publication Date: 2018-08-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

largely limits the wide application of these carotenoids

Method used

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  • Preparation method of gold nanoparticles, gold nanoparticles and application
  • Preparation method of gold nanoparticles, gold nanoparticles and application
  • Preparation method of gold nanoparticles, gold nanoparticles and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Bacterial culture and preparation of nano gold synthesis agent.

[0042] (1) Bacterial culture: Deinococcus radiodurans was plated and activated for culture, a single colony was picked and inoculated in 5mL TGY medium, and cultivated overnight at 32°C on a shaker with a shaker speed of 220rpm; then press 1:100 The proportion of the transfer to 1L culture flask (containing 500mL TGY medium), cultivated at 32 ℃, 220rpm for 24h to the bacterial stationary phase (OD 600nm = 1.0).

[0043] (2) Collecting and washing the bacteria: After the bacteria are cultivated to a stable period, they are collected by centrifugation at 8000 g for 10 minutes to obtain fresh bacteria. It was resuspended in 0.01M phosphate buffer solution with a pH of 7.2, fully shaken, centrifuged at 8000 g for 10 min, and the cells were collected.

[0044] (3) Preparation of nano-gold synthesis agent: shake the collected bacteria with pre-cooled acetone / methanol solvent (2 / 1, v / v) for 30 minutes at room ...

Embodiment 2

[0046] Bacterial culture and preparation of nano gold synthesis agent.

[0047] (1) Bacterial culture: Deinococcus radiodurans was plated and activated for culture, a single colony was picked and inoculated in 5mL TGY medium, and cultivated overnight at 30°C on a shaker with a shaker speed of 200rpm; then press 1:100 The proportion of the transfer to 1L culture flask (containing 500mL TGY medium), cultivated at 30 ℃, 200rpm for 24h to the bacterial stationary phase (OD 600nm = 1.2).

[0048] (2) Collecting and washing the bacterial cells: After the bacteria are cultivated to a stable period, they are collected by centrifugation at 10000 g for 8 minutes to obtain fresh bacterial cells. It was resuspended in 0.01M phosphate buffer solution with a pH of 7.2, shaken thoroughly, centrifuged at 10000 g for 8 min, and the cells were collected.

[0049] (3) Preparation of nano-gold synthetic agent: shake the collected bacteria with pre-cooled acetone / ethanol solvent (4 / 1, v / v) for 3...

Embodiment 3

[0051] Preparation of gold nanoparticles based on hydroxylated carotenoids.

[0052] (1) Preparation of synthetic reaction solution: dissolve 1.5 mg of radioresistant deinococcus (prepared in Example 1) in 10 mL of ethanol / water solvent (1 / 9, v / v), add 1 mM Au 3+ Solution (auric chloride, HAuCl 4 ·3H 2 O), at room temperature (pH 7, 25°C) to make it fully mixed and reacted.

[0053] (2) Monitoring the reaction process: As the reaction proceeds, the color of the solution changes and gradually tends to be stable. After 2 hours of reaction, a wavelength scan is performed (there is a peak at 539 nm).

[0054] (3) Purification of nano-gold solution: filter the above-mentioned red-purple solution with a 0.22 μm syringe filter, centrifuge at 15000 g for 60 min, collect the precipitate, wash with distilled water several times (the water solubility of nano-gold is better), and use a cut-off of 8KD The dialysis bag was dialyzed for 24 hours to remove the remaining reactants, and the ...

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Abstract

The invention discloses a preparation method of gold nanoparticles, the gold nanoparticles and application. The preparation method comprises the following steps that (1) a deinococcus radiodurans rhzomorph solution is provided; (2) an Au3+-containing solution is provided; (3) the solutions in the step (1) and the step (2) are mixed to react; and (4) after reaction is completed, products are collected and purified, and the gold nanoparticles are obtained. The method for preparing the gold nanoparticles has the advantages of simpleness, convenience, controllability, high efficiency, energy conservation and environmental friendliness, and the synthesized gold nanoparticles are good in water solubility, high in purity degree, good in uniformity, moderate in size (29.83+ / -4.18 nm), high in stability and high in antioxidant capacity, have the anticancer effect, and can be applied to the aspect of oxidation resistance or cancer inhibition.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing nano-gold, nano-gold and its application. Background technique [0002] Carotenoids, namely C 40 Hydrocarbons and their oxygen-containing derivatives are two major types of pigments, which are commonly found in photosynthetic bacteria, algae, plants and some non-photosynthetic bacteria. Hydroxylated carotenoids include astaxanthin, zeaxanthin, lutein and other oxygen-containing carotenoids, which have strong anti-oxidation, free radical scavenging, immune enhancement, and tumor prevention. Features. However, carotenoids with strong lipophilicity are easily soluble in organic solvents such as chloroform, acetone, benzene or carbon disulfide, and almost insoluble in water. To a large extent, the wide application of these carotenoids is limited. Studies have found that the pigment combined with nano-metal particles has better water solubility, more prominent c...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00B82Y30/00B82Y40/00C12P23/00C12R1/01
CPCC12P23/00B82Y30/00B82Y40/00B22F9/24B22F1/054
Inventor 田兵李九龙
Owner ZHEJIANG UNIV
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