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Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction

A radioresistant coccus, radiation-induced technology, applied in the directions of oxidoreductase, biochemical equipment and methods, enzymes, etc., can solve the problem of SOD carrying pathogens and other problems, and achieve short fermentation culture period, fast strain reproduction, and technological process. simple effect

Inactive Publication Date: 2005-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the SOD currently on the market uses animal blood as the raw material, and SOD from animal sources may carry the danger of pathogens, such as the mad cow disease that is prevalent in Europe

Method used

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  • Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction
  • Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Taking a dose of 1000Gy radiation to induce 1L bacterial cell culture and recovering culture for 30 minutes as an example.

[0026] 1 Streak-inoculate radiodurans D.radiodurans R1 on TGY solid medium, incubate at 32°C for 45 hours, pick a single colony and inoculate it in 100ml culture medium, and culture it in shake flask for 30 hours. 600 >1.0). The liquid seeds were inserted into 1L of fermentation medium according to the inoculum amount of 5%, and cultured at 32° C. with a stirring rotation speed of 200 rpm. Cultivate for 42 hours until the colony count > 10 8 / ml.

[0027] 2 Divide 1L of bacterial culture into polyethylene tubes (γ-rays can penetrate), and place in Co 60 Under radiation irradiation, the dose rate of 1000Gy / h is irradiated for 1 hour, and the total dose of the bacteria receiving irradiation treatment is 1000Gy.

[0028] 3. The cells induced by radiation were cultured at 32°C for 30 minutes, and the conditions were the same as above. ...

Embodiment 2

[0030] Example 2: Take 200Gy dose of radiation to induce 1L bacterial cell culture, and resume culture for 30 minutes as an example.

[0031] 1 Streak inoculation of radiodurans D.radiodurans R1 on TGY solid medium, culture at 30°C for 48 hours, pick a single colony and inoculate it in a Erlenmeyer flask containing 100ml of culture solution, shake the flask for 30 hours, and the growth of the bacteria reaches the desired level. Phases (OD 600 >1.0). The liquid seeds were inserted into 1L of fermentation medium according to the inoculum amount of 5%, and cultured at 32° C. with a stirring rotation speed of 200 rpm. Cultivate for 40 hours until the colony count > 10 8 / ml.

[0032] 2 Divide 1L of bacterial culture into polyethylene tubes (γ-rays can penetrate), and place in Co 60 Under radiation irradiation, a dose rate of 1000Gy / h was irradiated for 12 minutes, and the total dose of the bacteria received irradiation treatment was 200Gy.

[0033] 3. The cells induced by rad...

Embodiment 3

[0035] Example 3: Take 500Gy dose of radiation to induce 1L bacterial cell culture, and resume culture for 60 minutes as an example.

[0036] 1 Streak-inoculate radiodurans D.radiodurans R1 on TGY solid medium, culture at 32°C for 48 hours, pick a single colony and inoculate it in a triangular flask containing 100ml of culture solution, shake the flask for 30 hours, and the growth of the bacteria reaches the logarithmic phase (OD 600 >1.0). The liquid seeds were inserted into 1L fermentation medium according to the inoculum amount of 3%, cultured at 32°C, and the stirring speed was 220rpm. Cultivate for 38 hours until the colony count > 10 8 / ml.

[0037] 2 Divide 1L of bacterial culture into polyethylene tubes (γ-rays can penetrate), and place in Co 60 Under radiation irradiation, a dose rate of 1000Gy / h was irradiated for 30 minutes, and the total dose of the bacteria received irradiation treatment was 500Gy.

[0038] 3. The cells induced by radiation were cultured at 3...

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Abstract

The invention discloses a method for increasing the superoxide dismutase production of Radiation Coccus radiodurans induced by radiation, inducing the bacterial superoxide dismutase SOD by irradiating and post-culturing the radioresistant coccus radiodurans cultured to the stable early stage through gamma ray irradiation Synthesis, increase the production of bacterial enzymes. The bacterial cells of the present invention are separated, ultrasonically treated and extracted, and the obtained SOD enzyme activity is increased by more than 75% compared with that before irradiation treatment, which is 3 times that of the SOD-producing yeast enzyme activity. At the same time, the process flow is simple and the cost is low. The advantages.

Description

technical field [0001] The invention relates to a method for increasing the production of superoxide dismutase of radioresistant coccus by radiation induction. Background technique [0002] Superoxide dismutase (SOD) is an important antioxidant enzyme in organisms. The physiological function of SOD is to generate hydrogen peroxide and oxygen molecules through the disproportionation reaction of superoxide anion free radicals to prevent superoxide anion free radicals o 2 - Oxidative damage to biological macromolecules in the body. SOD is mainly used in medicine, health care products and cosmetics such as delaying human aging, preventing pigmentation caused by ultraviolet radiation, and removing local inflammation. . [0003] At present, there are three main sources of SOD preparation: ① superoxide dismutase in animals (cattle and horse blood); ② SOD from plants (such as tea, cabbage); ③ SOD from microorganisms. Most of the SOD currently on the mar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/02C12N13/00
Inventor 华跃进田兵
Owner ZHEJIANG UNIV
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