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Application of protein ibmyc2 in regulation of plant drought resistance

A technology of protein and drought resistance, applied in the application field of protein IbMYC2 in regulating plant drought resistance, can solve problems such as difficult plant stress resistance

Active Publication Date: 2022-02-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of plant stress resistance traits, it is very difficult to improve the stress resistance of plants using traditional breeding methods. Segregation becomes the main factor limiting genetic engineering of plant stress resistance

Method used

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  • Application of protein ibmyc2 in regulation of plant drought resistance
  • Application of protein ibmyc2 in regulation of plant drought resistance
  • Application of protein ibmyc2 in regulation of plant drought resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, the acquisition of IbMYC2 gene

[0067] The steps of obtaining the IbMYC2 gene are as follows:

[0068] 1. The total RNA of the young leaves of the sweet potato line JS6-5 was extracted with a plant total RNA extraction kit, and the first-strand cDNA was reverse transcribed from the total RNA with the QuantScript RT Kit Quant cDNA Kit.

[0069] 2. Using the cDNA obtained in step 1 as a template, use primers MYC2-F: 5′-ATGGAAGAGATTCTTTCTTCATCTT-3′ and primer MYC2-R: 5′-TTAAGACTGCAATCTTCTAAGGATG-3′ to perform PCR amplification to obtain a 1419bp PCR Products are amplified and sequenced.

[0070] The results showed that the nucleotide sequence of the PCR amplification product was shown as SEQ ID NO.1. The gene shown in SEQ ID NO.1 is named IbMYC2 gene, the protein encoded by it is named IbMYC2 protein or protein IbMYC2, and the amino acid sequence is shown in SEQ ID NO.2.

Embodiment 2

[0071] Example 2, Application of IbMYC2 Protein in Regulating Arabidopsis Drought Resistance

[0072] 1. Construction of recombinant plasmids

[0073] 1. Digest the vector pCAMBIA super1300-GFP with restriction endonucleases KpnI and SalI, and recover the 10783bp vector backbone.

[0074] 2. Artificially synthesize the double-stranded DNA molecule shown in SEQ ID NO.1. Using the double-stranded DNA molecule as a template, the primer MYC2-OE-F: 5'-GG GGTACC ATGGAAGAGATTCTTTCTTCATCTT-3' (the underline is the recognition sequence of restriction endonuclease KpnI) and primer MYC2-OE-R: 5'-ACGC GTC GAC The primer pair consisting of AGACTGCAATCTTCTAAGGATGAC-3' (the underline is the recognition sequence of restriction endonuclease SalI) was amplified by PCR to obtain a double-stranded DNA molecule containing the recognition sequence of restriction endonuclease.

[0075] 3. Ligate the double-stranded DNA molecule containing the restriction endonuclease recognition sequence obtain...

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Abstract

The invention discloses the application of the protein IbMYC2 in regulating the drought resistance of plants. The amino acid sequence of the protein IbMYC2 provided by the present invention is shown in SEQ ID NO:2. Experiments have shown that overexpressing the IbMYC2 gene in Arabidopsis thaliana can improve the drought resistance of Arabidopsis thaliana, and the improvement of drought resistance is manifested in: increased survival rate under drought stress, increased root length, increased fresh weight, increased JA content, increased SOD enzyme activity, MDA content decreased and H 2 o 2 content decreased. Thus, the protein IbMYC2 can regulate plant drought tolerance. The invention has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of protein IbMYC2 in regulating plant drought resistance. Background technique [0002] Drought is the most important environmental factor affecting plant growth and crop yield. Drought has become a serious problem affecting agricultural production. The use of genetic engineering to improve the drought resistance of crops and improve the adaptability of crops and economic crops to adversity is a key and major issue that needs to be solved urgently in the cultivation of new varieties. In recent years, people have done a lot of research on the mechanism of plant response to drought and other adversity stresses from the perspectives of physiology, biochemistry, metabolism, ecology, genetics, and evolution, and accumulated a wealth of data. Especially with the development of molecular biology, people The ability to understand the mechanism of plant resistance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N1/21A01H6/20A01H6/00A01H5/06A01H5/00C12R1/01
CPCC07K14/415C12N15/8273
Inventor 翟红刘庆昌何绍贞赵宁赵宏媛
Owner CHINA AGRI UNIV
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