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Novel application of Deinococcus radiodurans PprI protein and its pharmaceuticals

A protein and drug technology, applied in the use of PprI protein and the field of drugs, can solve problems such as drug efficacy and toxicity

Inactive Publication Date: 2016-01-27
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In recent years, the research on anti-radiation damage drugs has achieved remarkable results, but there are still many problems, the main problem is the efficacy and toxicity of drugs

Method used

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  • Novel application of Deinococcus radiodurans PprI protein and its pharmaceuticals
  • Novel application of Deinococcus radiodurans PprI protein and its pharmaceuticals
  • Novel application of Deinococcus radiodurans PprI protein and its pharmaceuticals

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Experimental program
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Effect test

Embodiment 1

[0050] Construction of embodiment 1pHBM-PprI expression vector and purification of PprI protein

[0051] According to the codon preference of Pichia pastoris, 40 pairs of DNA primers were designed and synthesized, the sequences of which are shown in SEQIDNO: 1-40. Primers 5'-gtcacatcatcaccaccatcatgttccatctgctaacgtttctccac-3' and 5'-ggccattattgggcagcatcttgtggttca-3' added a 6×His tag at their N-terminus (see figure 1 ), the sequence of the synthetic pprI gene is shown in SEQ ID NO: 81. The sequences of primers and genes are listed in Table 1.

[0052] Table 1 The sequences of primers and genes

[0053]

[0054]

[0055]

[0056] After purification, the PCR product was cloned into the CopI and NotI sites of the Pichia pastoris expression vector pHBM-905A (gifted by Professor Ma Lixin of Hubei University) to construct the recombinant expression plasmid pHBM905A-PprI ( figure 2 ). The plasmid pHBM905A-PprI was linearized with SalI enzyme, and then electrotransformed ...

Embodiment 2

[0058] Embodiment 2PprI protein anti-radiation effect test

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Abstract

The invention relates to the technical field of biology, in particular to an application of PprI protein and its pharmaceuticals. The application of the PprI protein in the preparation of pharmaceuticals for controlling radiation injury in higher eukaryotes is provided. Researches discover that the PprI protein is significant to controlling acute radiation injury in human cells and mice and enhances the antioxidant ability and DNA damage repairing ability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application and medicine of PprI protein. Background technique [0002] While the application of ionizing radiation in industry, agriculture, national defense and medicine brings great benefits to human society, large doses of ionizing radiation from nuclear accidents, tumor radiotherapy, spaceflight and other nuclear emergencies can cause serious human injury. Acute Radiation Injuries (ARIs) and cause very high mortality. The effective prevention and treatment of ARIs is a huge challenge in the field of international radiation injury. It is related to national nuclear safety and has been highly concerned by the governments of Europe and the United States. The treatment and protection of radiation injury has become a research hotspot in various countries. Therefore, the research and development of anti-radiation damage drugs has received great attention in modern medicine and he...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P39/00A61P39/06
CPCA61K38/16
Inventor 杨占山施怡任丽丽岳凌杨巍张舒羽
Owner SUZHOU UNIV
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