Application of berberine and doxorubicine mixed preparation in preparation of medicament against doxorubicine cardiac dysfunction or tumor
An anti-tumor drug, anti-doxorubicin technology, used in anti-tumor drugs, drug combinations, pharmaceutical formulations and other directions
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Embodiment 1
[0014] Example 1 Effect of berberine on cardiomyocyte injury induced by doxorubicin
[0015] One-day-old newborn SD suckling mice (provided by the Experimental Animal Center of Southern Medical University) were taken, and the apex of the heart was routinely digested with trypsin (Sigma, USA) to obtain cardiomyocytes according to the existing method.
[0016] Suspend the cells in DMEM (purchased from Gibico) containing 10% fetal bovine serum (purchased from Hyclone) and place at 37°C in 5% CO 2 In the incubator, incubate for 2 hours, and purify the obtained cardiomyocytes. According to 1×10 5 cells / mL were seeded in 96-well plates and cultured for 48 hours. 10 mg doxorubicin (DOX) (Sigma, USA) was dissolved in 5 mL of normal saline, and then diluted with DMEM (purchased from Gibico) medium to obtain a 10 μmol / L doxorubicin working solution. Berberine (Ber) (Sigma, USA) was dissolved in double-distilled water to 4.00 μmol / L working solution, and then diluted in DMEM to 1.00 μ...
Embodiment 2
[0027] Example 2 Effect of berberine on the viability of adriamycin-injured cardiomyocytes
[0028] The apical part of the apex of the SD suckling mouse was taken from 1-day-old newborn mice, and the cardiomyocytes were obtained by trypsinization. Suspend cells in DMEM containing 10% fetal bovine serum at 37°C, 5% CO 2 In the incubator, incubate for 2 hours, and purify the obtained cardiomyocytes. According to 1×10 5 cells / mL were seeded in 96-well plates and cultured for 48 hours. Dissolve doxorubicin injection (DOX) with 5 mL of normal saline, and then dilute with DMEM medium to 10 μmol / L doxorubicin working solution. Berberine (Ber) was dissolved in double distilled water to 4.00 μmol / L working solution, and then diluted to 1.00 μmol / L, 0.25 μmol / L, and 0.625 μmol / L working solution respectively. The purified cardiomyocytes were treated with the following drugs:
[0029] ①Control group: 200μL DMEM
[0030] ②Adriamycin (1 μmol / L) group: 180 μL DMEM+20 μL DOX
[0031] ...
Embodiment 3
[0042] Example 3 Observing the Effect of Berberine on Adriamycin-induced Myocardial Apoptosis
[0043] The apical part of the apex of the SD suckling mouse was taken from 1-day-old newborn mice, and the cardiomyocytes were obtained by trypsinization. Suspend the cells in DMEM (purchased from Gibico) containing 10% fetal bovine serum (purchased from Hyclone) and place at 37°C in 5% CO 2 In the incubator, incubate for 2 hours, and purify the obtained cardiomyocytes. According to 1×10 5 cells / mL were seeded in 96-well plates and cultured for 48 hours. Dissolve doxorubicin injection (DOX) with 5 mL of normal saline, and then dilute with DMEM medium to 10 μmol / L doxorubicin working solution. Berberine (Ber) was dissolved in double distilled water to 4.00 μmol / L working solution, and then diluted to 1.00 μmol / L, 0.25 μmol / L, and 0.625 μmol / L working solution respectively. After 48 hours of culture, cardiomyocytes were treated with the following drugs:
[0044] ①Control group: 2...
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