Medium for umbilical cord MSCs (mesenchymal stem cells)

Abstract

The invention discloses a medium for umbilical cord MSCs (mesenchymal stem cells). The medium is prepared from the following components: a basal medium, human serum albumin, myricetin, panax notoginsenoside, emodin, linoleic acid, trypsin, aprotinin, lumican, basic fibroblast growth factors, folic acid, beta-mercaptoethanol, astragalus polysaccharides, transferrin, vitamins and amino acid. The medium is compounded from the basal medium, human serum albumin, myricetin, panax notoginsenoside, emodin, linoleic acid, trypsin, aprotinin, photoprotective glycoprotein, the basic fibroblast growth factors, folic acid, beta-mercaptoethanol, astragalus polysaccharides, transferrin, the vitamins and the amino acid, the components play a synergistic role, and accordingly, the umbilical cord MSCs can be successfully cultured, proliferation of the umbilical cord MSCs is promoted, the growth rate of the umbilical cord MSCs is increased, activity of the umbilical cord MSCs during in-vitro proliferation and differentiation is improved, and the apoptosis rate is decreased.

Description

technical field

[0001] The invention belongs to the field of biotechnology, in particular to a culture medium for umbilical cord mesenchymal stem cells. Background technique

[0002] Umbilical cord mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) refer to a kind of multifunctional stem cells existing in the umbilical cord tissue of newborns, which can differentiate into many kinds of tissue cells and have broad clinical application prospects.

[0003] Umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions. It has broad clinical application prospects in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. It has been reported that mesenchymal stem cells were isolated from human umbilical cord, and the cell content and proliferation ability were superior to those of bone marrow mesenchymal stem cells, the immunogenicity was lower than that of bone mar...

Examples

Embodiment 1

[0066] Basal medium 24g / L, human serum albumin 12g / L, myricetin 12mg / L, Panax notoginseng saponins 12mg / L, emodin 13mg / L, linoleic acid 6mg / L, trypsin 5mg / L, aprotinin Enzyme 8mg / L, photoprotein glycan 13μg / L, basic fibroblast growth factor 5μg / L, folic acid 8mg / L, β-mercaptoethanol 6mg / L, astragalus polysaccharide 68mg / L, transferrin 5mg / L, Vitamin 280mg / L, amino acid 260mg / L.

[0067] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.

Embodiment 2

[0069] Basal medium 10g / L, human serum albumin 15g / L, myricetin 10mg / L, Panax notoginseng saponins 15mg / L, emodin 10mg / L, linoleic acid 2mg / L, trypsin 10mg / L, aprotinin Enzyme 10mg / L, photoprotein glycan 10μg / L, basic fibroblast growth factor 1μg / L, folic acid 15mg / L, β-mercaptoethanol 10mg / L, astragalus polysaccharide 80mg / L, transferrin 1mg / L, Vitamin 360mg / L, amino acid 180mg / L.

[0070] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.

Embodiment 3

[0072] Basal medium 50g / L, human serum albumin 5g / L, myricetin 5mg / L, Panax notoginseng saponins 20mg / L, emodin 5mg / L, linoleic acid 8mg / L, trypsin 1mg / L, aprotinin Enzyme 4mg / L, photoprotein glycan 20μg / L, basic fibroblast growth factor 8μg / L, folic acid 5mg / L, β-mercaptoethanol 2mg / L, astragalus polysaccharide 50mg / L, transferrin 3mg / L, Vitamin 500mg / L, amino acid 80mg / L.

[0073] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.

technical field

[0001] The invention belongs to the field of biotechnology, in particular to a culture medium for umbilical cord mesenchymal stem cells. Background technique

[0002] Umbilical cord mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) refer to a kind of multifunctional stem cells existing in the umbilical cord tissue of newborns, which can differentiate into many kinds of tissue cells and have broad clinical application prospects.

[0003] Umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions. It has broad clinical application prospects in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. It has been reported that mesenchymal stem cells were isolated from human umbilical cord, and the cell content and proliferation ability were superior to those of bone marrow mesenchymal stem cells, the immunogenicity was lower than that of bone mar...

Examples

Embodiment 1

[0066] Basal medium 24g / L, human serum albumin 12g / L, myricetin 12mg / L, Panax notoginseng saponins 12mg / L, emodin 13mg / L, linoleic acid 6mg / L, trypsin 5mg / L, aprotinin Enzyme 8mg / L, photoprotein glycan 13μg / L, basic fibroblast growth factor 5μg / L, folic acid 8mg / L, β-mercaptoethanol 6mg / L, astragalus polysaccharide 68mg / L, transferrin 5mg / L, Vitamin 280mg / L, amino acid 260mg / L.

[0067] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.

Embodiment 2

[0069] Basal medium 10g / L, human serum albumin 15g / L, myricetin 10mg / L, Panax notoginseng saponins 15mg / L, emodin 10mg / L, linoleic acid 2mg / L, trypsin 10mg / L, aprotinin Enzyme 10mg / L, photoprotein glycan 10μg / L, basic fibroblast growth factor 1μg / L, folic acid 15mg / L, β-mercaptoethanol 10mg / L, astragalus polysaccharide 80mg / L, transferrin 1mg / L, Vitamin 360mg / L, amino acid 180mg / L.

[0070] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.

Embodiment 3

[0072] Basal medium 50g / L, human serum albumin 5g / L, myricetin 5mg / L, Panax notoginseng saponins 20mg / L, emodin 5mg / L, linoleic acid 8mg / L, trypsin 1mg / L, aprotinin Enzyme 4mg / L, photoprotein glycan 20μg / L, basic fibroblast growth factor 8μg / L, folic acid 5mg / L, β-mercaptoethanol 2mg / L, astragalus polysaccharide 50mg / L, transferrin 3mg / L, Vitamin 500mg / L, amino acid 80mg / L.

[0073] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.