Medium for umbilical cord MSCs (mesenchymal stem cells)
A mesenchymal stem cell and culture medium technology, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problem of low growth rate of umbilical cord mesenchymal stem cells, improve activity, reduce cell apoptosis rate, improve growth speed effect
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Embodiment 1
[0066] Basal medium 24g / L, human serum albumin 12g / L, myricetin 12mg / L, Panax notoginseng saponins 12mg / L, emodin 13mg / L, linoleic acid 6mg / L, trypsin 5mg / L, aprotinin Enzyme 8mg / L, photoprotein glycan 13μg / L, basic fibroblast growth factor 5μg / L, folic acid 8mg / L, β-mercaptoethanol 6mg / L, astragalus polysaccharide 68mg / L, transferrin 5mg / L, Vitamin 280mg / L, amino acid 260mg / L.
[0067] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.
Embodiment 2
[0069] Basal medium 10g / L, human serum albumin 15g / L, myricetin 10mg / L, Panax notoginseng saponins 15mg / L, emodin 10mg / L, linoleic acid 2mg / L, trypsin 10mg / L, aprotinin Enzyme 10mg / L, photoprotein glycan 10μg / L, basic fibroblast growth factor 1μg / L, folic acid 15mg / L, β-mercaptoethanol 10mg / L, astragalus polysaccharide 80mg / L, transferrin 1mg / L, Vitamin 360mg / L, amino acid 180mg / L.
[0070] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.
Embodiment 3
[0072] Basal medium 50g / L, human serum albumin 5g / L, myricetin 5mg / L, Panax notoginseng saponins 20mg / L, emodin 5mg / L, linoleic acid 8mg / L, trypsin 1mg / L, aprotinin Enzyme 4mg / L, photoprotein glycan 20μg / L, basic fibroblast growth factor 8μg / L, folic acid 5mg / L, β-mercaptoethanol 2mg / L, astragalus polysaccharide 50mg / L, transferrin 3mg / L, Vitamin 500mg / L, amino acid 80mg / L.
[0073] The above formula was used to prepare the medium, and the umbilical cord mesenchymal stem cells were cultured according to the conventional culture method.
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