Method for producing natural carotenoid by cultivating deinococcus radiodurans

Abstract

The invention provides a method for producing natural carotenoid by cultivating deinococcus radiodurans, which is obtained through collection of soybean whey, edulocration, adjusting of potential of hydrogen (pH), culture adding, sterilization, activation of strain, seed cultivation, fermenting and cultivation, wall-breaking of thallus, extraction of carotenoid and preparation of dried products. The method utilizes by-product of the soybean whey in a processing process of soya bean products such as bean curd to cultivate bacteria to ferment and produce the natural carotenoid, achieves waste recovery of the soybean whey, improves resource use efficiency, reduces pollution on environment caused by soya bean product processing enterprises, and is favorable for protecting environment. 229.8 mg carotenoid dried products can be obtained from per liter of the soybean whey fermentation broth and are 226.2% higher than carotenoid dried products obtained through a conventional cultivation fermenting extraction method. In the method, processing of acid-heating and wall-breaking is firstly conducted on somatic cells, then ethanol is used for extraction, and the extraction is complete and fast. The method is simple in production technology process, low in production cost. Products are free of toxic residue and high in safety.

Description

technical field

[0001] The invention belongs to the technical field of fermentation, and relates to a method for cultivating Deinococcus radiodurans and fermenting to produce natural carotenoids by using soybean product yellow pulp water. Background technique

[0002] Tofu is a traditional functional food originating from China, and yellow syrup is a by-product formed during the production of tofu and other soy products. According to research, the content of solids in the yellow pulp water is more than 1%, wherein the protein content is about 0.3%, the fat content is about 0.08%, and the reducing sugar content is about 0.15%. In addition, it also contains many soybean isoflavones, soybean saponins, etc. a functional ingredient. However, most soybean product enterprises directly discharge a large amount of yellow pulp into sewers and rivers during the processing process, which not only does not make full use of its nutritional value, forming a waste, but also directly discha...

Examples

Embodiment 1

[0018] Taking yellow pulp water, 1% glucose, 0.1% magnesium sulfate, and weakly acidic pH 6.8 medium for fermentation, acid heat breaking, and ethanol extraction as an example

[0019] (1) Collection and removal of yellow pulp water: After collecting yellow pulp water in the production process of tofu and other soybean products, centrifuge at 5000 rpm for 20 minutes at room temperature to remove impurities and solids.

[0020] (2) pH adjustment of yellow pulp water: yellow pulp water itself has strong acidity, adjust the pH value of yellow pulp water to 6.8 with 2.0mol / L NaOH.

[0021] (3) Sterilization of yellow pulp water: add 1% glucose and 0.1% magnesium sulfate to the yellow pulp water in (2) above, use high temperature and high pressure sterilization method, 121°C, 30 minutes, sterilize and fill until fermentation Fermentation culture in bottle as culture medium (SWG).

[0022] (4) Activation of strains, seed culture: Streak inoculation of Deinococcus radiodurans on the...

Embodiment 2

[0029] Take yellow pulp water, 0.5% glucose, 0.1% magnesium sulfate, weak alkaline pH7.3 medium fermentation culture, acid heat breaking, ethanol extraction as an example

[0030] (1) Collection and removal of yellow pulp water: After collecting yellow pulp water in the production process of tofu and other soybean products, centrifuge at 5000 rpm for 20 minutes at room temperature to remove impurities and solids.

[0031] (2) pH adjustment of yellow pulp water: yellow pulp water itself has strong acidity, adjust the pH value of yellow pulp water to 7.3 with 2.0mol / L NaOH.

[0032] (3) Sterilization of yellow pulp water: add 0.5% glucose and 0.1% magnesium sulfate to the yellow pulp water in (2) above, use high temperature and high pressure sterilization method, 121 ° C, 30 minutes, sterilize and fill until fermentation Fermentation culture in bottle as culture medium (SWG).

[0033](4) Activation of strains, seed culture: Streak inoculation of Deinococcus radiodurans on the s...

Embodiment 3

[0040] Take yellow pulp water, 1% glucose, 0.1% magnesium sulfate, medium fermentation culture of weak acid pH6.8, acid heat breaking, acetone / methanol extraction as an example

[0041] (1) Collection and removal of yellow pulp water: After collecting yellow pulp water in the production process of tofu and other soybean products, centrifuge at 5000 rpm for 20 minutes at room temperature to remove impurities and solids.

[0042] (2) pH adjustment of yellow pulp water: yellow pulp water itself has strong acidity, adjust the pH value of yellow pulp water to 7.3 with 2.0mol / L NaOH.

[0043] (3) Sterilization of yellow pulp water: Add 1% glucose and 0.1% magnesium sulfate to the yellow pulp water in (2) above, use high temperature and high pressure sterilization method, 121 ° C, 30 minutes, and fill it to Fermentation culture in fermentation flasks as culture medium (SWG).

[0044] (4) Activation of strains, seed culture: Streak inoculation of Deinococcus radiodurans on the seed m...

technical field

[0001] The invention belongs to the technical field of fermentation, and relates to a method for cultivating Deinococcus radiodurans and fermenting to produce natural carotenoids by using soybean product yellow pulp water. Background technique

[0002] Tofu is a traditional functional food originating from China, and yellow syrup is a by-product formed during the production of tofu and other soy products. According to research, the content of solids in the yellow pulp water is more than 1%, wherein the protein content is about 0.3%, the fat content is about 0.08%, and the reducing sugar content is about 0.15%. In addition, it also contains many soybean isoflavones, soybean saponins, etc. a functional ingredient. However, most soybean product enterprises directly discharge a large amount of yellow pulp into sewers and rivers during the processing process, which not only does not make full use of its nutritional value, forming a waste, but also directly discha...

Examples

Embodiment 1

[0018] Taking yellow pulp water, 1% glucose, 0.1% magnesium sulfate, and weakly acidic pH 6.8 medium for fermentation, acid heat breaking, and ethanol extraction as an example

[0019] (1) Collection and removal of yellow pulp water: After collecting yellow pulp water in the production process of tofu and other soybean products, centrifuge at 5000 rpm for 20 minutes at room temperature to remove impurities and solids.

[0020] (2) pH adjustment of yellow pulp water: yellow pulp water itself has strong acidity, adjust the pH value of yellow pulp water to 6.8 with 2.0mol / L NaOH.

[0021] (3) Sterilization of yellow pulp water: add 1% glucose and 0.1% magnesium sulfate to the yellow pulp water in (2) above, use high temperature and high pressure sterilization method, 121°C, 30 minutes, sterilize and fill until fermentation Fermentation culture in bottle as culture medium (SWG).

[0022] (4) Activation of strains, seed culture: Streak inoculation of Deinococcus radiodurans on the...

Embodiment 2

[0029] Take yellow pulp water, 0.5% glucose, 0.1% magnesium sulfate, weak alkaline pH7.3 medium fermentation culture, acid heat breaking, ethanol extraction as an example

[0030] (1) Collection and removal of yellow pulp water: After collecting yellow pulp water in the production process of tofu and other soybean products, centrifuge at 5000 rpm for 20 minutes at room temperature to remove impurities and solids.

[0031] (2) pH adjustment of yellow pulp water: yellow pulp water itself has strong acidity, adjust the pH value of yellow pulp water to 7.3 with 2.0mol / L NaOH.

[0032] (3) Sterilization of yellow pulp water: add 0.5% glucose and 0.1% magnesium sulfate to the yellow pulp water in (2) above, use high temperature and high pressure sterilization method, 121 ° C, 30 minutes, sterilize and fill until fermentation Fermentation culture in bottle as culture medium (SWG).

[0033](4) Activation of strains, seed culture: Streak inoculation of Deinococcus radiodurans on the s...

Embodiment 3

[0040] Take yellow pulp water, 1% glucose, 0.1% magnesium sulfate, medium fermentation culture of weak acid pH6.8, acid heat breaking, acetone / methanol extraction as an example

[0041] (1) Collection and removal of yellow pulp water: After collecting yellow pulp water in the production process of tofu and other soybean products, centrifuge at 5000 rpm for 20 minutes at room temperature to remove impurities and solids.

[0042] (2) pH adjustment of yellow pulp water: yellow pulp water itself has strong acidity, adjust the pH value of yellow pulp water to 7.3 with 2.0mol / L NaOH.

[0043] (3) Sterilization of yellow pulp water: Add 1% glucose and 0.1% magnesium sulfate to the yellow pulp water in (2) above, use high temperature and high pressure sterilization method, 121 ° C, 30 minutes, and fill it to Fermentation culture in fermentation flasks as culture medium (SWG).

[0044] (4) Activation of strains, seed culture: Streak inoculation of Deinococcus radiodurans on the seed m...

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