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Deinococcus wushiensis and application thereof in cobalt ion biological adsorption treatment

An adsorption and application technology, applied in microorganism-based methods, microorganisms, water/sewage treatment, etc., can solve the problems of low removal rate, high operating cost, secondary pollution, etc.

Inactive Publication Date: 2013-07-17
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the main method for treating cobalt-containing waste liquid is the sulfidation precipitation method [Chen Weiping, Yang Qingshan, He Bining, et al. Research on the recovery of cobalt in NdFeB waste. Rare metals and hard Alloy, 2006, 34: 55-57], ammonium oxalate precipitation method [Tan Haixiang, Hu Qiyang, Li Xinhai, etc. A new process for cobalt recovery from lithium cobalt oxide waste pole pieces. Power Technology, 2007, 31:288-230], hydrochloric acid reaction Phase extraction method [Shuai Guoquan. Recovery of cobalt in Jinchuan Company. Nonferrous Smelting, 1995(3): 15-18] and other methods, these treatment methods have achieved good results to a certain extent, but secondary pollution is common, especially when the concentration of heavy metals in water is low (<100 mg / L ), not only the removal rate is low, but also the operating cost is high; at present, there is no relevant report on the adsorption of cobalt ions

Method used

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  • Deinococcus wushiensis and application thereof in cobalt ion biological adsorption treatment
  • Deinococcus wushiensis and application thereof in cobalt ion biological adsorption treatment
  • Deinococcus wushiensis and application thereof in cobalt ion biological adsorption treatment

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Embodiment 1

[0021] Embodiment one: Deinococcus urica ( Deinococcus wushiensis ) Screening, classification and identification of RV12 CGMCC No.6675

[0022] A heavy metal-resistant strain RV12 was isolated and screened from a radiation-contaminated environment in an arid desert area in Xinjiang; it was found through morphological, physiological and biochemical identification that its colony was orange-red, small, round, and small after being cultured in TGY medium for 5 days. The edges are neat, the surface is smooth and opaque; the cells are round, in the form of doublets or quadruples; the cells are Gram-positive, non-motile, aerobic, and the optimum growth temperature is 30°C, and the optimum growth pH is 7.0. Sucrose, sorbitol, maltose, mannose, melezitose, xylose, arabinose, rhamnose, dextrin, mannitol, cystine, proline, threonine, tryptophan, tyrosine acid as the only carbon source, the main fatty acid is C16: 1ω7c (53.2%), C16: 0 (20.0%), C16: 1ω9c (6.0%), and the main quinone is...

Embodiment 2

[0024] Embodiment two: Deinococcus urica ( Deinococcus wushiensis ) Liquid culture of RV12 CGMCC No.6675

[0025] The activated bacterial strain Deinococcus uricae of the present invention ( Deinococcus wushiensis) RV12 CGMCC No.6675 was inoculated in a seed test tube containing 5ml of TGY liquid medium, cultured at 30°C, shaken at 200rpm for 36 hours, then inoculated into a TGY liquid fermentation bottle at a 2% inoculation amount, and the amount added was 500ml Bottle 80ml of TGY liquid medium, culture at 30°C, shake at 220rpm for 60h; the surface of TGY medium is 5g of protein, 3g of yeast extract, 2g of glucose, 15g of agar, 1L of distilled water, pH7.5, and culture at 30°C for 48h.

[0026] After the above culture, the maximum concentration of the bacteria can reach 4.51 OD / ml (600nm); the culture is centrifuged at 8000 rpm for 3 minutes, the supernatant is discarded, and the bacteria are collected. The wet weight of the bacteria can reach 5.2g / L.

Embodiment 3

[0027] Embodiment three: Deinococcus urica ( Deinococcus wushiensis ) RV12 CGMCC No.6675 heavy metal resistance

[0028] The activated bacterial strain Deinococcus uricae of the present invention ( Deinococcus wushiensis ) RV12 CGMCC No.6675 was inoculated in a seed test tube containing 5ml of TGY liquid medium, cultured at 30°C, shaken at 200rpm for 36 hours, and then inoculated at 2% inoculum size in different concentrations of Hg 2+ 、Cu 2+ 、Co 2+ , Pb 2+ 、Cr 2+ In the TGY liquid fermentation bottle, add 80ml TGY liquid culture medium in a 500ml triangular flask, cultivate at 30°C, shake at 220rpm for 72h, and observe the growth of the strain; see the attached figure 2 , by attached figure 2 It can be drawn that the application bacterial strain Deinococcus urica ( Deinococcus wushiensis ) RV12 CGMCC No.6675 is resistant to various metals, among which Pb 2+ 、Cr 2+ The maximum tolerated concentration (100mg / L) ， Cu 2+ 、Co 2+ Followed by (80mg / L); the surface o...

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Abstract

The invention discloses Deinococcus wushiensis RV12CGMCC No.6675 and application thereof in cobalt ion biological adsorption treatment. A strain is inoculated to medium containing 5ml TGY (tryptone glucose yeast) liquid and cultured at 30 DEG C. After shaking culture at 200rpm for 36h, the strain is cultured according to 2% inoculated culture. The cultured strain is tolerant to Hg2+, Cu2+, Co2+, Pb2+ and Cr2+, the tolerance to the Pb2+ and the Cr2+ is up to 150mg / L and 100mg / L respectively, and the tolerance to the Cu2+ and the Co2+ is up to 80mg / L. The Deinococcus wushiensis is widely applicable to the field of environmental treatment of waste liquid containing heavy metals, and is especially important to the application in biological adsorption treatment of cobalt ions.

Description

technical field [0001] The invention relates to the technical field of heavy metal biosorption, in particular, the invention relates to the technical field of application of radiation-resistant bacteria in heavy metal ion adsorption. Background technique [0002] Heavy metals refer to specific gravity greater than 4g / cm 3 or 5g / cm 3 There are about 45 kinds of metals such as copper, zinc, lead, cobalt, manganese, cadmium, mercury, etc.; due to the increasing mining, smelting, processing and commercial manufacturing activities of heavy metals, a variety of heavy metals such as lead, mercury, cadmium, Copper, cobalt, etc. enter the atmosphere, water, and soil, and with the retention, accumulation, and migration of heavy metal ions in the environment or ecosystem, they cause serious environmental pollution and ecological hazards. [0003] Traditional physical and chemical methods for treating wastewater containing heavy metals include chemical precipitation, ion exchange, el...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F1/58C12R1/01C02F101/20
Inventor 张志东王玮朱静王成顾美英王浩陈一峰宋素琴唐琦勇张丽娟高升旗乔坤云
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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