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Feed additive for improving laying yield of female silkworm moths and preparation method thereof

A feed additive and production method technology, applied in microorganism-based methods, animal feed, animal feed, etc., can solve the problems of not very obvious effect, reduce the rate of infertile eggs, etc., achieve significant economic and social benefits, and improve production. The effect of the number of eggs, the number of eggs laid, and the stability of the product characteristics

Inactive Publication Date: 2012-08-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some studies have shown that: (1) Proper protection of temperature, humidity and light conditions during the pupal stage can reduce non-oviposition female moths and less-oviposition female moths, increase the number of eggs laid, reduce infertile eggs, and increase the rate of good eggs; (2) The larval stage lives in an environment rich in negative oxygen ions, which can speed up the oviposition rate, increase the total oviposition, improve the rate of good eggs, and reduce the rate of infertile eggs; (3) change the quality of mulberry leaves and add Eating amino acids, proteins, trace elements and hormones, etc., can increase the number of eggs and the rate of laying eggs of silkworm moths to a certain extent, but the effects of these methods are not very obvious.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: a kind of preparation method of the feed additive that improves the egg production of Bombyx moth female moth, carries out following steps successively:

[0026] 1), the crushing treatment of anti-radiation bacteria (D.radiopugnance),

[0027] The radiopugnance strain D. radiopugnance with the preservation number ATCC No. 19172 is provided by the American Type Culture Collection. The specific steps are:

[0028] ①, the activation of bacteria:

[0029] Streak-inoculate D.radiopugnance on TGY solid medium, culture at 30°C for 36 hours, pick a single colony and inoculate it in the TGY liquid medium in the Erlenmeyer flask, and culture at 30°C until logarithmic growth phase; Obtain activated seed bacterium liquid;

[0030] The preparation method of TGY liquid medium is as follows:

[0031] Add distilled water to 6 g of peptone, 3 g of yeast extract and 1 g of glucose to 1000 mL; then carry out conventional high-temperature sterilization (1.1 atmospheres, 1...

Embodiment 2

[0072] Embodiment 2: a kind of preparation method of the feed additive that improves the egg production of Bombyx moth female moth, carries out following steps successively:

[0073] 1), the crushing treatment of anti-radiation bacteria (D.radiopugnance),

[0074] The radiopugnance strain D. radiopugnance with the accession number of ATCC No. 19172 is provided by the American Type Culture Collection. The specific steps are:

[0075] ①, the activation of bacteria:

[0076] Streak inoculation of D.radiopugnance on the TGY solid medium, culture at 32°C for 34 hours, pick a single colony and inoculate it in the TGY liquid medium in the Erlenmeyer flask, and culture at 32°C until the logarithmic growth phase; Obtain activated seed bacterium liquid;

[0077] TGY liquid culture medium and TGY solid culture medium are the same as embodiment 1.

[0078] ②. Fermentation culture:

[0079] Put the activated seed bacterium solution obtained in step 1 into the culture medium (i.e. defa...

Embodiment 3

[0093] Embodiment 3: a kind of preparation method of the feed additive that improves the egg production of Bombyx moth female moth, carries out following steps successively:

[0094] 1), the crushing treatment of anti-radiation bacteria (D.radiopugnance),

[0095] The radiopugnance strain D. radiopugnance with the preservation number ATCC No. 19172 is provided by the American Type Culture Collection. The specific steps are:

[0096] ①, the activation of bacteria:

[0097] Streak-inoculate D.radiopugnance on the TGY solid medium, culture at 28°C for 38 hours, pick a single colony and inoculate it in the TGY liquid medium in the Erlenmeyer flask, and culture at 28°C until the logarithmic growth phase; Obtain activated seed bacterium liquid;

[0098] TGY liquid culture medium and TGY solid culture medium are the same as embodiment 1.

[0099] ②. Fermentation culture:

[0100] The activated seed bacterial liquid obtained in step 1. is inserted into the culture medium (i.e. de...

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Abstract

The invention discloses a feed additive for improving laying yield of female silkworm moths, which is formed by mixing deinococcus radiopugnance (D. radiopugnance) after being smashed and zinc sulfate heptahydrate (ZnSO4.7H2O) according to a mass ratio of 1: 0.95 to 1.05. The invention also provides a preparation method of the feed additive. The D. radiopugnance is smashed through the following steps that: seed inocula of the D. radiopugnance with a reservation number of ATCC 19172 is inoculated to a fermentation culture medium to be cultured, and the acquired fermentation liquid is processedfor 40 to 60s under the condition with the frequency of 600 to 700MHz and the electric field strength of 8000 to 9000V / em after being centrifuged; and finally the smashed D. radiopugnance is acquiredafter being dried.

Description

technical field [0001] The invention belongs to the technical field of silkworm feed additives, and relates to a feed additive for increasing the egg laying capacity of silkworm female moths and a preparation method thereof. Background technique [0002] The egg production of the silkworm female moth is one of the key factors determining the economic benefits of the silkworm breeding unit (silkworm farm). How to increase the egg production of the silkworm female moth is one of the current research hotspots of the silkworm breeding workers. The amount of eggs laid by silkworms is determined by the number of eggs produced and the efficiency of egg laying. A female moth with a large number of eggs lays more eggs, so the number of eggs laid is undoubtedly the most important factor in determining the number of eggs laid. Theoretically, a silkworm female moth can produce up to 800 silkworm eggs, but due to reasons such as nutrition, feeding or protection conditions, and mating, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16C12N1/20C12R1/01
Inventor 屠振力
Owner ZHEJIANG UNIV
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