Method for immobilizing deinococcus radiodurans with functional magnetic carriers and application of method

A technology of Deinococcus radioresistant and magnetic carrier, which is applied in the direction of fixation on or in the inorganic carrier, radioactive purification, nuclear engineering, etc., can solve the problems of poor mechanical strength, difficult separation, poor chemical stability, etc., and achieve the improvement of chemical Stability and mechanical strength, strong chemical stability, good radiation resistance

Inactive Publication Date: 2015-12-09
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently domestic and foreign DR There are few reports on bacteria remediation of water polluted by low-concentration uranium
in addition, DR Bacteria tend to grow in a suspended state in uranium-containing wastewater solutions, and the density difference between bacteria and water is small, making it difficult to separate from water, which affects the actual effect of repairing uranium-containing wastewater from uranium mining and smelting
So far, although there have been occasional reports on the immobilization of Deinococcus radiodurans, the immobilization materials used are mainly natural polymer gel carriers such as calcium alginate and sodium alginate, as well as polyacrylamide (PAM), polyethylene Organically synthesized polymer gel carriers such as alcohol (PVA), these carriers have high water solubility, poor chemical stability, and poor mechanical strength. At the same time, the fixed DR Bacteria are easy to fall off, and the impact resistance is not good, so that its practical application is limited, and there is a big gap with the requirements of actual engineering applications.

Method used

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  • Method for immobilizing deinococcus radiodurans with functional magnetic carriers and application of method
  • Method for immobilizing deinococcus radiodurans with functional magnetic carriers and application of method
  • Method for immobilizing deinococcus radiodurans with functional magnetic carriers and application of method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) DR Bacteria expansion and DR Preparation of bacteria

[0043] First, immobilized Deinococcus radiodurans (referred to as DR Bacteria) for activation culture, use the inoculation loop to pick DR Bacteria, and plated on the pre-prepared solid TGY medium (each 100mL TGY medium contains 0.5g of tryptone, 0.3g of yeast extract, 0.1g of glucose, 1.5g of agar, pH=7.0), and the well-marked The entire plate was transferred into a constant temperature shaking incubator (220r / min) at 30°C for 48h. Then use the inoculation loop to pick the activated culture DR Put the bacteria into the EP tube filled with liquid TGY medium (that is, no agar is added to the solid TGY medium), and move the inoculated EP tube to a constant temperature shaking incubator (220r / min) at 30°C for expanded culture for 72h . After the TGY medium is covered with mycelia, take out the EP tube and collect by centrifugation DR Bacteria (8000r / min, 10min), washed with deionized water (3 times), t...

Embodiment 2

[0054] (1) DR Bacteria expansion and DRPreparation of bacteria

[0055] First, immobilized Deinococcus radiodurans (referred to as DR Bacteria) for activation culture, use the inoculation loop to pick DR Bacteria, and plated on the pre-prepared solid TGY medium (each 100mL TGY medium contains 0.5g of tryptone, 0.3g of yeast extract, 0.1g of glucose, 1.5g of agar, pH=7.0), and the well-marked The entire plate was transferred into a constant temperature shaking incubator (220r / min) at 30°C for 48h. Then use the inoculation loop to pick the activated culture DR Put the bacteria into the EP tube filled with liquid TGY medium (that is, no agar is added to the solid TGY medium), and move the inoculated EP tube to a constant temperature shaking incubator (220r / min) at 30°C for expanded culture for 72h . After the TGY medium is covered with mycelia, take out the EP tube and collect by centrifugation DR Bacteria (8000r / min, 10min), washed with deionized water (3 times), th...

Embodiment 3

[0066] (1) DR Bacteria expansion and DR Preparation of bacteria

[0067] First, immobilized Deinococcus radiodurans (referred to as DR Bacteria) for activation culture, use the inoculation loop to pick DR Bacteria, and plated on the pre-prepared solid TGY medium (each 100mL TGY medium contains 0.5g of tryptone, 0.3g of yeast extract, 0.1g of glucose, 1.5g of agar, pH=7.0), and the well-marked The entire plate was transferred into a constant temperature shaking incubator (220r / min) at 30°C for 48h. Then use the inoculation loop to pick the activated culture DR Put the bacteria into the EP tube filled with liquid TGY medium (that is, no agar is added to the solid TGY medium), and move the inoculated EP tube to a constant temperature shaking incubator (220r / min) at 30°C for expanded culture for 72h . After the TGY medium is covered with mycelia, take out the EP tube and collect by centrifugation DR Bacteria (8000r / min, 10min), washed with deionized water (3 times), t...

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Abstract

The invention provides a method for immobilizing deinococcus radiodurans with functional magnetic carriers and an application of the method in treatment of uranium-bearing wastewater. An organic reagent is adopted for surface acyl chloride functionalization of magnetic Fe3O4 nanoparticles to obtain DR (deinococcus radiodurans) immobilizing carriers, the DR immobilizing carriers are magnetically immobilized with diethylenetriamine chemically-modified DR to obtain a functional magnetic adsorbent resistant to deinococcus radiodurans, and the functional magnetic adsorbent resistant to deinococcus radiodurans is applied to adsorption of the uranium-bearing wastewater. The novel functional magnetic adsorbent resistant to deinococcus radiodurans has the advantages of high radiation resistance, low cost, high chemical stability, high mechanical strength, effectiveness in uranium adsorption and the like. In addition, the method is simple and easy to realize magnetic control, and uranium content of low-concentration uranium-bearing wastewater with the concentration being 0.45-0.55mg/L can be reduced by more than 95%.

Description

technical field [0001] The invention belongs to the field of biological adsorption treatment of uranium-containing wastewater from uranium mining and metallurgy, and specifically relates to a method for immobilizing Deinococcus radiodurans with a novel functionalized magnetic carrier and its application in the treatment of uranium-containing wastewater. Background technique [0002] According to the strategic needs of the nuclear military industry and the development plan of nuclear power, in order to meet the growing demand for natural uranium in the nuclear military industry and nuclear power, my country will still increase the intensity of uranium mining and dressing. The uranium-containing wastewater produced in the process will also increase day by day. According to incomplete statistics, a certain uranium mine in southern my country produces as much as 300,000 tons of pit water every year. In addition to the immersion water of uranium tailings pond and uranium ore heap ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14G21F9/12
Inventor 肖方竹彭国文何淑雅黄波唐艳
Owner NANHUA UNIV
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