Efficient lead ion bio-adsorbent and preparation method and application thereof

A biosorbent, lead ion technology, applied in the field of environmental pollution control, can solve the problem that bacteria cannot survive well

Active Publication Date: 2015-06-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, under extreme environmental conditions (such as high oxidation environment), most bacteria cannot survive well, so it is difficult to be us

Method used

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  • Efficient lead ion bio-adsorbent and preparation method and application thereof
  • Efficient lead ion bio-adsorbent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: A kind of Pb derived from radiodurans 2+ The preparation method of biosorbent, the steps are as follows:

[0025] a. Bacterial culture, the prepared TGY medium (dissolve 5g peptone, 3g yeast powder and 1g glucose in 1 L water) was sterilized in an autoclave, the sterilization condition was 121°C, and the sterilization time was 20min. A single colony of Deinococcus radiodurans was picked from the plate and cultured overnight in a shaker in 5ml sterilized TGY medium, the culture condition was 30°C, and the shaker speed was 200rpm. Then it was transferred to the culture medium of a large bottle according to the inoculation ratio of 1%, and cultured at 30°C and 200rpm for 24h.

[0026] b. Collect the bacterial cells, cultivate the cells to the stationary phase, and collect the bacterial cells by centrifugation with a centrifugal force of 8000g and a centrifugation time of 10 minutes to obtain fresh bacterial cells.

[0027] c. Wash the bacteria, re-suspe...

Embodiment 2

[0030] Example 2: A kind of Pb derived from radiodurans 2+ The preparation method of biosorbent, the steps are as follows:

[0031] a. Bacterial culture, the prepared TGY medium (dissolve 5g peptone, 3g yeast powder and 1g glucose in 1 L water) was sterilized in an autoclave, the sterilization condition was 121°C, and the sterilization time was 20min. A single colony of Deinococcus radiodurans was picked from the plate and cultured overnight in a shaker in 5ml sterilized TGY medium, the culture condition was 30°C, and the shaker speed was 220rpm. Then it was transferred to the culture medium of a large bottle according to the inoculation ratio of 1%, and cultured at 30°C and 220rpm for 20h.

[0032] b. Collect the bacterial cells, cultivate the cells to the stationary phase, and collect the bacterial cells by centrifugation with a centrifugal force of 8000g and a centrifugation time of 10 minutes to obtain fresh bacterial cells.

[0033] c. Wash the bacteria, re-suspend t...

Embodiment 3

[0036] Example 3: A kind of Pb derived from radiodurans 2+ The preparation method of biosorbent, the steps are as follows:

[0037] a. Bacterial culture, the prepared TGY medium (dissolve 5g peptone, 3g yeast powder and 1g glucose in 1 L water) was sterilized in an autoclave, the sterilization condition was 121°C, and the sterilization time was 20min. A single colony of Deinococcus radiodurans was picked from the plate and cultured overnight in a shaker in 5ml sterilized TGY medium, the culture condition was 30°C, and the shaker speed was 200rpm. Then it was transferred to the culture medium of a large bottle according to the inoculation ratio of 1%, and cultured at 30°C and 180rpm for 28h.

[0038] b. Collect the bacterial cells, cultivate the cells to the stationary phase, and collect the bacterial cells by centrifugation with a centrifugal force of 8000g and a centrifugation time of 10 minutes to obtain fresh bacterial cells.

[0039] c. Wash the bacteria, re-suspend t...

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Abstract

The invention discloses an efficient lead ion bio-adsorbent and a preparation method and application thereof. The bio-adsorbent contains deinococcus radiodurans which is obtained from the American Type Culture Collection Center (ATCC) with a collection number of ATCC13939, the pH value of a Pb<2+>-containing solution to be treated is 2.0-4.0, and the concentration of the deinococcus radiodurans is 0.02-0.04 g/ml after the bio-adsorbent is added. The pH value of the Pb<2+>-containing solution to be treated is regulated to 2.0-4.0, then the bio-adsorbent is added, the final concentration of the adsorbent is 0.02-0.04 g/ml, stirring reaction is carried out for 3 minutes or more, then Pb<2+> in a water body is removed, and the strains are separated from the solution by filtration, centrifugation or natural precipitation. The water body has radioactivity or has the temperature higher than 50 DEG C.

Description

technical field [0001] The invention belongs to the field of environmental pollution control, and relates to a biosorbent using bacteria and a method for efficiently and quickly removing lead ions in a solution. Background technique [0002] Due to the development of modern industry and human activities, heavy metal pollution has become increasingly serious around the world, and heavy metal pollution incidents have also occurred frequently in my country, such as blood lead poisoning and cadmium rice pollution incidents. These events have sounded the alarm to us, and the accumulation of heavy metals in the environment has caused serious threats to human health. [0003] The control methods of heavy metal pollution mainly include physical methods, chemical methods and biological methods. Among them, physical methods and chemical methods have become the main methods for research and application because of their simple operation and easy access to materials. Recently, the use ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01J20/24C02F3/34G21F9/18C12R1/01
CPCC02F3/34C02F2101/20C12N1/20C12N1/205C12R2001/01G21F9/18
Inventor 华跃进李涛田兵
Owner ZHEJIANG UNIV
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