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Preparation method of nano-gold, nano-gold and application

A nano-gold, reaction technology, applied in the field of bioengineering, to achieve the effect of wide adaptability and moderate size

Active Publication Date: 2018-04-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the synthesis of gold nanoparticles from bacterial extracts.

Method used

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  • Preparation method of nano-gold, nano-gold and application
  • Preparation method of nano-gold, nano-gold and application
  • Preparation method of nano-gold, nano-gold and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Bacterial culture and preparation of nano gold synthesis agent.

[0035] (1) Bacterial culture: Deinococcus radiodurans with the preservation number ATCC13939 was plated and activated for culture, then a single colony was picked and inoculated in 5 mL of TGY medium, and cultivated overnight at 32°C on a shaker with a shaker speed of 220rpm then transferred to a large bottle of medium (1L culture bottle containing 500mL TGY medium) in a ratio of 1:100, and cultivated for 24h at 32°C and 220rpm to make the bacteria culture to the stationary phase (OD600nm=1.0).

[0036] (2) Collecting and washing the bacteria: After the bacteria are cultivated to a stable period, they are collected by centrifugation at 8000 g for 10 minutes to obtain fresh bacteria. It was resuspended in 0.01M phosphate buffer solution with a pH of 7.0, fully shaken, centrifuged at 8000 g for 10 min, and the cells were collected.

[0037] (3) Disintegrating the cells and preparing cell extracts: resuspen...

Embodiment 2

[0040] Bacterial culture and preparation of nano gold synthesis agent.

[0041] (1) Bacterial culture: Deinococcus radiodurans with the preservation number ATCC13939 was plated and activated for culture, then a single colony was picked and inoculated in 5 mL of TGY medium, and cultivated overnight at 30°C on a shaker with a shaker speed of 220rpm Then transfer to large bottle medium (1L culture bottle containing 500mL TGY medium) in the ratio of 1: 100, cultivate 24h under the condition of 30 ℃, 220rpm and make bacterial culture to stationary phase (OD600nm=1.0).

[0042] (2) Collecting and washing the bacteria: After the bacteria are cultivated to a stable period, they are collected by centrifugation at 8000 g for 10 minutes to obtain fresh bacteria. It was resuspended in 0.01M phosphate buffer solution with a pH of 7.0, fully shaken, centrifuged at 8000 g for 10 min, and the cells were collected.

[0043](3) Disintegrating the cells and preparing cell extracts: resuspend th...

Embodiment 3

[0046] Preparation of gold nanoparticles.

[0047] (1) Preparation of synthetic reaction solution: 1mM Au 3+ Solution (auric chloride, HAuCl 4 ·3H 2 O) In a 50mL centrifuge tube, add an appropriate amount of Deinococcus radiodurans extract dry powder (prepared in Example 1, without ammonium sulfate treatment) so that the concentration is 3.00mg / mL, at room temperature (pH 7, 25°C) Make it fully mixed and reacted under the conditions.

[0048] (2) Monitoring the reaction process: As the reaction proceeds, the color of the solution changes, gradually becomes purple and then tends to be stable. After 6 hours of reaction, the wavelength scanning is performed (there is a peak at 545nm).

[0049] (3) Purification of nano-gold solution: filter the above-mentioned purple solution with a 0.22 μm syringe filter, centrifuge at 15000 g for 30 min, collect the precipitate, wash with distilled water several times, and dialyze for 24 h with a dialysis bag with a cutoff of 14 KD to remove ...

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Abstract

The invention discloses a preparation method of nano-gold, the nano-gold and application. The preparation method of the nano-gold comprises the following steps: (1) after crushing deinococcus radiodurans thalli, taking out supernatant to prepare a nano-gold synthesis agent; (2) adding a solution containing Au<3+> into the nano-gold synthesis agent and reacting; (3) collecting sediment and washingto obtain the nano-gold. According to the preparation method disclosed by the invention, extract of deinococcus radiodurans is used for preparing the nano-gold and the nano-gold can be efficiently, greenly and conveniently synthesized; the synthesized nano-gold has good purity and a moderate size (about 51.72nm); the surface of the nano-gold has negative electricity and the nano-gold basically hasno cytotoxicity; the deinococcus radiodurans extract is used as a metal nano material and has the advantages which do not belong to common plant and bacterium extract.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing nano-gold, nano-gold and its application. Background technique [0002] Gold is a rare and precious metal with stable chemical properties. Due to its unique physical and chemical properties, it plays a unique and novel role in decoration, electronics, equipment, and biomedical engineering. [0003] Nanogold refers to tiny particles of gold with a diameter of 1-100nm. It has high electron density, dielectric properties and catalytic effect. It can be combined with a variety of biological macromolecules without affecting its biological activity. It has a wide range of applications in the fields of sensing, biochips, optics, and biomedicine. Gold nanoparticles of various particle sizes can be conveniently prepared by the reduction method from chloroauric acid, and its color is red to purple depending on the diameter. [0004] Generally speaking, nanom...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/01
CPCC12P3/00
Inventor 田兵李九龙
Owner ZHEJIANG UNIV
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