Establishment method for type 2 diabetes mellitus animal model

Abstract

The invention discloses an establishment method for a type 2 diabetes mellitus animal model. The establishment method comprises the following steps: (1) preparing a lipopolysaccharide solution with aconcentration of 100ug/ml by virtue of normal saline; (2) selecting male rats which are 4-6 weeks old, feeding by virtue of a common feed, and simultaneously injecting the lipopolysaccharide solution;(3) weighing the male rats after 28 days; (4) after the weighing, fasting for 8 hours, separating serum, and measuring blood glucose and insulin; (5) evaluating the resistance of the insulin and glucose stimulated insulin secretion capacity; (6) respectively intraperitoneally injecting an STZ solution into the male rats in the 30th day and the 32th day; and (7) determining that the establishmentof the type 2 diabetes mellitus animal model is finished when the random blood sugar is more than 16.8mmol/L. According to the establishment method, lipopolysaccharide in Escherichia coli 0111:B4 subspecies is selected and is matched with the feeding of the common feed and the STZ injection so as to successfully establish the type 2 diabetes mellitus animal mode; according to the establishment method, the operation is easy, the model establishment cost is low, the model establishment rate is high, and the model establishment period is short.

Description

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AI Technical Summary

Benefits of technology

The technical effect of this new technology compared to previous methods for inducing weight loss was that it's easier to use without requiring expensive equipment or having long cycles between different steps. This makes it more efficient at producing animals suitable for commercial sale while reducing costs associated therewith.

Problems solved by technology

Technological Problem addressed in this patents relates to improving the accuracy and efficiency of predictive medicine studies conducted through rodent populations during clinical trials. Current rat testing systems require expensive equipment and complicated procedures, making them difficult to apply widely across different types of medical treatments. There exists a demand for improved nonclindrance diabete rats capable of accurately simulating various metabolism states associated with diabetese individuals who may suffer from diabets alone.

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