3 results about "Manis" patented technology

The application discloses a pangolin species identification method based on nail piece element characteristics, which comprises the following steps: step S1: collecting nail piece samples covering existing 10 kinds of pangolin species, and obtaining initial data of multi-element characteristics of the nail piece samples; step S2: identifying the species of the nail piece samples by a molecular biology method, and obtaining the species labels of each nail piece sample; step S3: constructing an "element characteristic-species label" standard data set; step S4: selecting a table prior fitting network as a core machine learning algorithm, training a model by using training set data, and constructing the model; and step S5: identifying whether a to-be-detected sample belongs to a pangolin nail piece according to morphological judgment. The method has a single sample data collection time of less than 60 seconds, does not need to damage the sample, is used for nail piece identification of samples without prior clues, and the overall accuracy of a global ten-species classification model reaches 0.93.

The invention belongs to the technical field of molecular markers, and particularly relates to a multiple fluorescent quantitative PCR (polymerase chain reaction) detection method and a kit for distinguishing Chinese pangolin, manis pentadactyla and South African pangolin varieties. Three groups of specific primers and probes are utilized to distinguish different varieties of pangolin scales, including Chinese pangolin scales, malayan pangolin scales and South African pangolin scales. Specific gene segments of three species are amplified at the same time through one qPCR reaction, and the method has the advantages of being high in specificity and sensitivity, easy and convenient to operate and low in cost. The method is suitable for pangolin species identification, forensic identification, customs examination and biological protection research.

The invention belongs to the technical field of molecular biology, and particularly relates to a multiplex PCR primer combination for identifying species of pangolin and application of the multiplex PCR primer combination. Three pairs of specific primers are designed aiming at species specific conserved regions in three kinds of pangolin mitochondrial genes and respectively target COI genes of Chinese pangolin, pangolin males and pangolin south African. By utilizing the primer group, synchronous identification of three pangolin species can be realized in the same PCR reaction system, and visual interpretation is performed through agarose gel electrophoresis according to the size of an amplified fragment. The detection method disclosed by the invention has the advantages of rapidness, accuracy, strong specificity, simplicity and convenience in operation and the like, is suitable for species identification of squama manis scales, tissues, blood and products thereof, and provides an efficient and reliable technical means for illegal trade traceability and species protection monitoring and identification of the squama manis.