Method for constructing model of animal with chronic cardiac failure heart qi deficiency and blood stasis edema

Abstract

The invention discloses a method for constructing a model of an animal with chronic cardiac failure heart qi deficiency and blood stasis edema. The method includes the following steps that a clean adult male SD rat is selected, the rat is placed in a cleansing cage, the temperature ranges from 19 DEG C to 23 DEG C, the relative humidity ranges from 40% to 50%, a natural lighting mode is adopted, and the rat is fed through the rat standard feeds and can drink water freely; after adaptive feeding is conducted for one week, lavage is conducted through adriamycin of 0.2 % of the fodder weight, and propylthiouracil injection is conducted on the abdominal cavity one time every week according to the proportion of 3.5 mg/kg and continues for six weeks. The method for constructing the model of the animal with the chronic cardiac failure heart qi deficiency and the blood stasis edema has the advantages that the model can better simulate the chronic cardiac failure heart qi deficiency and the blood stasis edema, meet the pathological characters, for example, LVEF and LVFS reduce remarkably, and BNP increases, of the chronic cardiac failure in western medicine, and show the chronic cardiac failure heart qi deficiency and the blood stasis edema in traditional Chinese medicine, and the copy method is good in stability and repeatability, simple and objective.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing an animal model of chronic heart failure with syndromes of heart-qi deficiency combined with blood stasis and edema combined with disease and syndrome. Background technique [0002] Chronic heart failure is a clinically complex syndrome that can be stable, decompensated, or worsen. The theory of traditional Chinese medicine believes that heart failure is mainly located in the heart, but it is related to the other four viscera. deficit. However, the soil of the spleen is born in the fire of the heart, and the child's disease can reach the mother, so the spleen's unhealthy movement can induce and aggravate the occurrence of heart failure. [0003] In the early stage of the evolution of the TCM syndrome law of chronic heart failure, it first manifests as deficiency of heart qi or deficiency of heart qi. Symptoms include palpitation, shortness o...

AI Technical Summary

Problems solved by technology

Although doxorubicin is injected intraperitoneally into rats every other day to create a chronic heart failure model, it cannot fully simulate the symptoms of chronic heart failure with heart-qi deficiency and blood stasis and edema, such as chest pain, chest tightness, breathlessness, mass under the flank, edema, oliguria, and cold Cold limbs etc.

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